Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,776 T→G 6.2% intergenic (‑16/+52) ycjD ← / ← sapF DUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,7760TG 6.2% 469.2 / 14.7 154intergenic (‑16/+52)ycjD/sapFDUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (71/73);  new base G (4/6);  total (75/79)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 157 total aligned reads displayed.

TCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACAT  >  NC_000913/1351628‑1351925
                                                                                                                                                    |                                                                                                                                                     
tctccACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGAtt                                                                                                                                                      <  2:1915214/147‑1 (MQ=255)
 ccccACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGAttt                                                                                                                                                     >  2:737491/1‑150 (MQ=255)
   ccACTGGATGTTGACGCCGAAATTTGAAAACACTAAAACGTCGGCAGCGAAGATATCGCCAGAGCTTTCGTACCTGCAAAGTGAGATTGCGTCTTAAATAACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTaa                                                                                                                                                   >  2:143006/1‑150 (MQ=255)
    cACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAAt                                                                                                                                                  >  1:861076/1‑150 (MQ=255)
    cACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAAt                                                                                                                                                  <  2:232877/150‑1 (MQ=255)
    cACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTGCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAAt                                                                                                                                                  <  2:1814349/150‑1 (MQ=255)
      cTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCt                                                                                                                                                >  2:2037013/1‑150 (MQ=255)
      atGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCt                                                                                                                                                <  2:126025/149‑1 (MQ=255)
       tGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCtt                                                                                                                                               >  2:1642456/1‑150 (MQ=255)
        ggATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCttt                                                                                                                                              >  1:1391495/1‑150 (MQ=255)
          aTGTTGTCGGCGAAATTTGAAATCACTAACACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGCCAAGATTTAATCTTTTg                                                                                                                                            <  2:1307610/150‑1 (MQ=255)
          aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCTGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGAATGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                            <  1:346820/150‑1 (MQ=255)
          aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                            >  1:1114469/1‑150 (MQ=255)
           tGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGt                                                                                                                                           >  2:1734400/1‑150 (MQ=255)
            gTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTc                                                                                                                                          <  2:1607766/150‑1 (MQ=255)
             ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTTCAAAGTGCGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                         <  2:2012741/150‑1 (MQ=255)
             ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                         <  2:1551047/150‑1 (MQ=255)
             ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                         <  2:1750084/150‑1 (MQ=255)
             ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                         >  2:1034446/1‑150 (MQ=255)
              tGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAg                                                                                                                                        <  1:1625610/150‑1 (MQ=255)
              tGACGGCGAAATTTGAAATCACTAAAACGTCGGCAGCGAAGATATCGCCAGAGATTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCAGTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATGTAATCTTTTGTCat                                                                                                                                        >  2:258702/1‑149 (MQ=255)
               gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                       >  1:152651/1‑150 (MQ=255)
                 cGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAAt                                                                                                                                     >  2:162234/1‑150 (MQ=255)
                  ggCGAAATTTGAACTCACTAAAACGTCGGCTGGGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                    <  1:309238/150‑1 (MQ=38)
                  ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                    <  1:632232/150‑1 (MQ=38)
                  ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATACCGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATg                                                                                                                                    >  2:1889746/1‑150 (MQ=255)
                   gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGa                                                                                                                                   <  2:601074/150‑1 (MQ=38)
                    cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAg                                                                                                                                  >  1:1465339/1‑150 (MQ=255)
                    cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGAGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAg                                                                                                                                  <  2:1832220/150‑1 (MQ=38)
                     gAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCTCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAgg                                                                                                                                 <  1:909469/150‑1 (MQ=38)
                       aaTTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGATTGAGGTg                                                                                                                               >  1:1858481/1‑150 (MQ=255)
                         tttGAAATCACTAAAACGTCGGCTGCGAAGATATAGCCCGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATATAATTTTATCTATCACCTCATTCTGACAAGATTTAATCGTTAGGCAGAATGATGTGaa                                                                                                                             >  2:1019389/1‑150 (MQ=255)
                             aaaTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGaataat                                                                                                                         >  1:399371/1‑150 (MQ=255)
                               aTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCt                                                                                                                       >  1:54442/1‑150 (MQ=255)
                                 cACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg                                                                                                                     >  2:193995/1‑150 (MQ=255)
                                  aCTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGa                                                                                                                    >  1:23560/1‑150 (MQ=255)
                                     aaaaCGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAagg                                                                                                                 >  1:780772/1‑150 (MQ=255)
                                         cGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAggagga                                                                                                             <  2:842091/150‑1 (MQ=255)
                                               cTGAGAAGATATCGCCAGATCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGa                                                                                                       <  2:1071107/150‑1 (MQ=255)
                                                  cGAAGATTTCGCCAGAGGTTTCGTTCCTGCATTGTGAGTTTGCGTCTTAAATCACGCGCATTTGATTTAAATTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                    <  1:187858/150‑1 (MQ=255)
                                                  cGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                    >  1:226542/1‑150 (MQ=255)
                                                  cGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGAGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                    <  1:2038260/150‑1 (MQ=255)
                                                   gAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCCTTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAAt                                                                                                   <  2:1844173/150‑1 (MQ=255)
                                                      tatatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAg                                                                                                <  2:1574423/149‑1 (MQ=255)
                                                        tatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCg                                                                                              >  2:1488475/1‑150 (MQ=255)
                                                        tatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCg                                                                                              <  1:1435563/150‑1 (MQ=255)
                                                          tCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGAt                                                                                            >  1:565767/1‑150 (MQ=255)
                                                           cGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAATTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCCGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGATGATTCAGAAAATTAGCGAta                                                                                           >  2:406323/1‑149 (MQ=255)
                                                            gCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCt                                                                                          >  1:587528/1‑150 (MQ=255)
                                                             ccAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCtt                                                                                         >  1:1307521/1‑150 (MQ=255)
                                                              cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAACTCACGCGCATTTGAGTTAATTTTATCTATCAACTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTCGAATGAGGATTCAGAAAAATAGCGATCttt                                                                                        >  1:178802/1‑150 (MQ=255)
                                                              cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                        <  1:767887/150‑1 (MQ=255)
                                                              cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                        <  1:1480449/150‑1 (MQ=255)
                                                                            cTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATccg                                                                          >  1:1596711/1‑150 (MQ=255)
                                                                                  aGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCa                                                                    >  2:88440/1‑150 (MQ=255)
                                                                                      agaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCTCCGTCAATGc                                                                >  1:979693/1‑150 (MQ=255)
                                                                                      agaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGc                                                                <  2:1629377/150‑1 (MQ=255)
                                                                                       gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                               <  1:902794/150‑1 (MQ=255)
                                                                                          tGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCg                                                            >  2:1479682/1‑150 (MQ=255)
                                                                                            cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                          <  2:399371/150‑1 (MQ=255)
                                                                                            cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                          <  1:1034447/150‑1 (MQ=255)
                                                                                            cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                          <  1:1244283/150‑1 (MQ=255)
                                                                                               cTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGTCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCaaa                                                       >  2:914309/1‑150 (MQ=255)
                                                                                                 tAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAAt                                                     >  2:1181477/1‑150 (MQ=255)
                                                                                                  aaaTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATg                                                    <  1:436148/150‑1 (MQ=255)
                                                                                                    aTCACGCTCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCATAATGATGCGATTAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATCCGCCGTCAATGCCTCGCCAAAATGAc                                                  <  2:1026306/150‑1 (MQ=255)
                                                                                                    aTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAATATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGAc                                                  <  1:411059/150‑1 (MQ=255)
                                                                                                       aCGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCgg                                               <  2:494214/150‑1 (MQ=255)
                                                                                                        cgcgGATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                              <  1:606244/150‑1 (MQ=255)
                                                                                                        cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                              <  2:1639765/150‑1 (MQ=255)
                                                                                                        cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                              <  1:759748/150‑1 (MQ=255)
                                                                                                                tGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGcc                                      <  1:1919009/150‑1 (MQ=255)
                                                                                                                  aTTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGt                                    >  1:487488/1‑150 (MQ=255)
                                                                                                                     tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCTGGCATCCGCCGTCAATGGCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                 <  1:1019390/150‑1 (MQ=255)
                                                                                                                     tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                 >  1:681141/1‑150 (MQ=255)
                                                                                                                       aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                               <  2:95815/150‑1 (MQ=255)
                                                                                                                       aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                               >  2:1434532/1‑150 (MQ=255)
                                                                                                                       aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                               >  2:1370511/1‑150 (MQ=255)
                                                                                                                        ttttATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTg                              <  2:1790547/150‑1 (MQ=255)
                                                                                                                        ttttATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTg                              >  1:781048/1‑150 (MQ=255)
                                                                                                                              ctatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCa                        >  1:440924/1‑150 (MQ=255)
                                                                                                                               tatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTTTAAGGAGGATTCAGAAAATTAGCTATCTTTACGCCACGCATCCTACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTctt                       >  1:1075196/1‑148 (MQ=255)
                                                                                                                                atcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGGGAGTTCATg                      <  2:1292594/150‑1 (MQ=255)
                                                                                                                                 tcACCTCATTCTGACAAGATTTAATCTTTTGTGAGAATGAGGTGAATAATCTGGATGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCATTGCCTCGCCAAAATGACCGTCAATCAGCCGTTTGGTGAGTTCATGc                     <  1:1740949/150‑1 (MQ=255)
                                                                                                                                 tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCCCGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                     >  2:529271/1‑150 (MQ=255)
                                                                                                                                 tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                     <  1:1488476/150‑1 (MQ=255)
                                                                                                                                  cACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCa                    <  2:905858/150‑1 (MQ=255)
                                                                                                                                    ccTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgc                  >  2:1715308/1‑150 (MQ=255)
                                                                                                                                      tCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgcgg                <  1:1218626/150‑1 (MQ=255)
                                                                                                                                         ttCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATACAGCGGCGa             >  2:727020/1‑150 (MQ=255)
                                                                                                                                            tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc          >  1:1576713/1‑150 (MQ=255)
                                                                                                                                            tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc          <  1:1906192/150‑1 (MQ=255)
                                                                                                                                              aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa        <  2:487488/150‑1 (MQ=255)
                                                                                                                                              aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa        <  2:54442/150‑1 (MQ=255)
                                                                                                                                               cAAGATTTAATCTTTTGTCAGAATGAGTTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCGCGCATCCGCCGTCAATGCCTCGCCAAAATGAACGGCAATCAGCCGTTTGGTGAGTTCAAGCAGCGGCGATGCCAg       >  2:1254955/1‑150 (MQ=255)
                                                                                                                                               cAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAg       >  2:1683289/1‑150 (MQ=255)
                                                                                                                                                aaGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGc      <  2:226542/150‑1 (MQ=255)
                                                                                                                                                aaGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGc      <  2:1117051/150‑1 (MQ=255)
                                                                                                                                                aaGATTTAATCTTTTGGCAGAATGAGGTGAATAATCTGGAAGGAGGAGTCAGAAAATTAGCGATCTTTACGCCACGCAGCCGCCGTCAATGCCTCGCCAAAAAGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCTGc      <  1:2003161/150‑1 (MQ=255)
                                                                                                                                                    tttAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTTAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATAAGCCGTTTGGTGTGTTCATGAAGCGGCGATGCCAGaacaa  >  2:166722/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACAT  >  NC_000913/1351628‑1351925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: