Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,779 A→T 6.4% intergenic (‑19/+49) ycjD ← / ← sapF DUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,7790AT 6.4% 446.7 / 14.6 156intergenic (‑19/+49)ycjD/sapFDUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (73/73);  new base T (4/6);  total (77/79)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

Only 100 of 157 total aligned reads displayed.

CCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCC  >  NC_000913/1351631‑1351927
                                                                                                                                                    |                                                                                                                                                    
ccACTGGATGTTGACGCCGAAATTTGAAAACACTAAAACGTCGGCAGCGAAGATATCGCCAGAGCTTTCGTACCTGCAAAGTGAGATTGCGTCTTAAATAACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTaa                                                                                                                                                     >  2:143006/1‑150 (MQ=255)
 cACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAAt                                                                                                                                                    >  1:861076/1‑150 (MQ=255)
 cACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAAt                                                                                                                                                    <  2:232877/150‑1 (MQ=255)
 cACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTGCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAAt                                                                                                                                                    <  2:1814349/150‑1 (MQ=255)
   cTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCt                                                                                                                                                  >  2:2037013/1‑150 (MQ=255)
   atGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCt                                                                                                                                                  <  2:126025/149‑1 (MQ=255)
    tGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCtt                                                                                                                                                 >  2:1642456/1‑150 (MQ=255)
     ggATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCttt                                                                                                                                                >  1:1391495/1‑150 (MQ=255)
       aTGTTGTCGGCGAAATTTGAAATCACTAACACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGCCAAGATTTAATCTTTTg                                                                                                                                              <  2:1307610/150‑1 (MQ=255)
       aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCTGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGAATGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                              <  1:346820/150‑1 (MQ=255)
       aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                              >  1:1114469/1‑150 (MQ=255)
        tGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGt                                                                                                                                             >  2:1734400/1‑150 (MQ=255)
         gTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTc                                                                                                                                            <  2:1607766/150‑1 (MQ=255)
          ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTTCAAAGTGCGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                           <  2:2012741/150‑1 (MQ=255)
          ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                           >  2:1034446/1‑150 (MQ=255)
          ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                           <  2:1551047/150‑1 (MQ=255)
          ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                           <  2:1750084/150‑1 (MQ=255)
           tGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAg                                                                                                                                          <  1:1625610/150‑1 (MQ=255)
           tGACGGCGAAATTTGAAATCACTAAAACGTCGGCAGCGAAGATATCGCCAGAGATTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCAGTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATGTAATCTTTTGTCat                                                                                                                                          >  2:258702/1‑149 (MQ=255)
            gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                         >  1:152651/1‑150 (MQ=255)
            gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                         >  2:785753/1‑150 (MQ=255)
              cGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAAt                                                                                                                                       >  2:162234/1‑150 (MQ=255)
               ggCGAAATTTGAACTCACTAAAACGTCGGCTGGGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                      <  1:309238/150‑1 (MQ=38)
               ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                      <  1:632232/150‑1 (MQ=38)
                gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGa                                                                                                                                     <  2:601074/150‑1 (MQ=38)
                 cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAg                                                                                                                                    >  1:1465339/1‑150 (MQ=255)
                 cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGAGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAg                                                                                                                                    <  2:1832220/150‑1 (MQ=38)
                  gAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCTCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAgg                                                                                                                                   <  1:909469/150‑1 (MQ=38)
                    aaTTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGATTGAGGTg                                                                                                                                 >  1:1858481/1‑150 (MQ=255)
                      tttGAAATCACTAAAACGTCGGCTGCGAAGATATAGCCCGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATATAATTTTATCTATCACCTCATTCTGACAAGATTTAATCGTTAGGCAGAATGATGTGaa                                                                                                                               >  2:1019389/1‑150 (MQ=255)
                          aaaTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGaataat                                                                                                                           >  1:399371/1‑150 (MQ=255)
                            aTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCt                                                                                                                         >  1:54442/1‑150 (MQ=255)
                              cACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg                                                                                                                       >  2:193995/1‑150 (MQ=255)
                               aCTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGa                                                                                                                      >  1:23560/1‑150 (MQ=255)
                                  aaaaCGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAagg                                                                                                                   >  1:780772/1‑150 (MQ=255)
                                            cTGAGAAGATATCGCCAGATCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGa                                                                                                         <  2:1071107/150‑1 (MQ=255)
                                               cGAAGATTTCGCCAGAGGTTTCGTTCCTGCATTGTGAGTTTGCGTCTTAAATCACGCGCATTTGATTTAAATTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                      <  1:187858/150‑1 (MQ=255)
                                               cGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                      >  1:226542/1‑150 (MQ=255)
                                               cGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGAGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                      <  1:2038260/150‑1 (MQ=255)
                                                gAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCCTTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAAt                                                                                                     <  2:1844173/150‑1 (MQ=255)
                                                   tatatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAg                                                                                                  <  2:1574423/149‑1 (MQ=255)
                                                     tatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCg                                                                                                <  1:1435563/150‑1 (MQ=255)
                                                     tatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCg                                                                                                >  2:1488475/1‑150 (MQ=255)
                                                       tCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGAt                                                                                              >  1:565767/1‑150 (MQ=255)
                                                        cGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAATTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCCGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGATGATTCAGAAAATTAGCGAta                                                                                             >  2:406323/1‑149 (MQ=255)
                                                         gCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCt                                                                                            >  1:587528/1‑150 (MQ=255)
                                                          ccAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCtt                                                                                           >  1:1307521/1‑150 (MQ=255)
                                                           cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAACTCACGCGCATTTGAGTTAATTTTATCTATCAACTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTCGAATGAGGATTCAGAAAAATAGCGATCttt                                                                                          >  1:178802/1‑150 (MQ=255)
                                                           cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                          <  1:767887/150‑1 (MQ=255)
                                                           cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                          <  1:1480449/150‑1 (MQ=255)
                                                                         cTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATccg                                                                            >  1:1596711/1‑150 (MQ=255)
                                                                            cAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATccgccg                                                                         >  2:1888364/1‑150 (MQ=255)
                                                                               aGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCa                                                                      >  2:88440/1‑150 (MQ=255)
                                                                                   agaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCTCCGTCAATGc                                                                  >  1:979693/1‑150 (MQ=255)
                                                                                   agaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGc                                                                  <  2:1629377/150‑1 (MQ=255)
                                                                                    gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                 <  1:902794/150‑1 (MQ=255)
                                                                                    gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                 <  2:1891495/150‑1 (MQ=255)
                                                                                       tGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCg                                                              >  2:1479682/1‑150 (MQ=255)
                                                                                         cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                            <  2:399371/150‑1 (MQ=255)
                                                                                         cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                            <  1:1034447/150‑1 (MQ=255)
                                                                                         cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                            <  1:1244283/150‑1 (MQ=255)
                                                                                            cTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGTCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCaaa                                                         >  2:914309/1‑150 (MQ=255)
                                                                                              tAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAAt                                                       >  2:1181477/1‑150 (MQ=255)
                                                                                               aaaTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATg                                                      <  1:436148/150‑1 (MQ=255)
                                                                                                 aTCACGCTCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCATAATGATGCGATTAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATCCGCCGTCAATGCCTCGCCAAAATGAc                                                    <  2:1026306/150‑1 (MQ=255)
                                                                                                 aTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAATATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGAc                                                    <  1:411059/150‑1 (MQ=255)
                                                                                                    aCGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCgg                                                 <  2:494214/150‑1 (MQ=255)
                                                                                                     cgcgGATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                <  1:606244/150‑1 (MQ=255)
                                                                                                     cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                <  2:1639765/150‑1 (MQ=255)
                                                                                                     cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                <  1:759748/150‑1 (MQ=255)
                                                                                                             tGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGcc                                        <  1:1919009/150‑1 (MQ=255)
                                                                                                               aTTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGt                                      >  1:487488/1‑150 (MQ=255)
                                                                                                                  tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCTGGCATCCGCCGTCAATGGCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                   <  1:1019390/150‑1 (MQ=255)
                                                                                                                  tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                   >  1:681141/1‑150 (MQ=255)
                                                                                                                    aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                 >  2:1370511/1‑150 (MQ=255)
                                                                                                                    aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                 <  2:95815/150‑1 (MQ=255)
                                                                                                                    aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                 >  2:1434532/1‑150 (MQ=255)
                                                                                                                     ttttATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTg                                <  2:1790547/150‑1 (MQ=255)
                                                                                                                     ttttATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTg                                >  1:781048/1‑150 (MQ=255)
                                                                                                                           ctatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCa                          >  1:440924/1‑150 (MQ=255)
                                                                                                                            tatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTTTAAGGAGGATTCAGAAAATTAGCTATCTTTACGCCACGCATCCTACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTctt                         >  1:1075196/1‑148 (MQ=255)
                                                                                                                             atcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGGGAGTTCATg                        <  2:1292594/150‑1 (MQ=255)
                                                                                                                              tcACCTCATTCTGACAAGATTTAATCTTTTGTGAGAATGAGGTGAATAATCTGGATGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCATTGCCTCGCCAAAATGACCGTCAATCAGCCGTTTGGTGAGTTCATGc                       <  1:1740949/150‑1 (MQ=255)
                                                                                                                              tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCCCGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                       >  2:529271/1‑150 (MQ=255)
                                                                                                                              tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                       <  1:1488476/150‑1 (MQ=255)
                                                                                                                               cACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCa                      <  2:905858/150‑1 (MQ=255)
                                                                                                                                 ccTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgc                    >  2:1715308/1‑150 (MQ=255)
                                                                                                                                   tCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgcgg                  <  1:1218626/150‑1 (MQ=255)
                                                                                                                                      ttCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATACAGCGGCGa               >  2:727020/1‑150 (MQ=255)
                                                                                                                                         tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc            >  1:1576713/1‑150 (MQ=255)
                                                                                                                                         tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc            <  1:1906192/150‑1 (MQ=255)
                                                                                                                                           aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa          <  2:487488/150‑1 (MQ=255)
                                                                                                                                           aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa          <  2:54442/150‑1 (MQ=255)
                                                                                                                                            cAAGATTTAATCTTTTGTCAGAATGAGTTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCGCGCATCCGCCGTCAATGCCTCGCCAAAATGAACGGCAATCAGCCGTTTGGTGAGTTCAAGCAGCGGCGATGCCAg         >  2:1254955/1‑150 (MQ=255)
                                                                                                                                            cAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAg         >  2:1683289/1‑150 (MQ=255)
                                                                                                                                             aaGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGc        <  2:226542/150‑1 (MQ=255)
                                                                                                                                             aaGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGc        <  2:1117051/150‑1 (MQ=255)
                                                                                                                                             aaGATTTAATCTTTTGGCAGAATGAGGTGAATAATCTGGAAGGAGGAGTCAGAAAATTAGCGATCTTTACGCCACGCAGCCGCCGTCAATGCCTCGCCAAAAAGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCTGc        <  1:2003161/150‑1 (MQ=255)
                                                                                                                                                 tttAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTTAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATAAGCCGTTTGGTGTGTTCATGAAGCGGCGATGCCAGaacaa    >  2:166722/1‑149 (MQ=255)
                                                                                                                                                   tAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATcc  >  2:885230/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCC  >  NC_000913/1351631‑1351927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: