Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,782 C→A 6.3% intergenic (‑22/+46) ycjD ← / ← sapF DUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,7820CA 6.3% 456.2 / 15.5 155intergenic (‑22/+46)ycjD/sapFDUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (75/70);  new base A (4/6);  total (79/76)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 157 total aligned reads displayed.

ACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCCGCGG  >  NC_000913/1351633‑1351931
                                                                                                                                                     |                                                                                                                                                     
aCTTGATGTTGACGGCGAAATTTTAAATCACTAAAACGTCGGCTGCGAAGATATCGACAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTAGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATc                                                                                                                                                       <  1:856696/150‑1 (MQ=255)
 atGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCt                                                                                                                                                      <  2:126025/149‑1 (MQ=255)
   ggATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCttt                                                                                                                                                    >  1:1391495/1‑150 (MQ=255)
     aTGTTGTCGGCGAAATTTGAAATCACTAACACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGCCAAGATTTAATCTTTTg                                                                                                                                                  <  2:1307610/150‑1 (MQ=255)
     aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCTGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGAATGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                                  <  1:346820/150‑1 (MQ=255)
     aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                                  >  1:1114469/1‑150 (MQ=255)
     aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                                  <  2:1941621/150‑1 (MQ=255)
      tGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGt                                                                                                                                                 >  2:1734400/1‑150 (MQ=255)
        ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTTCAAAGTGCGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                               <  2:2012741/150‑1 (MQ=255)
        ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                               >  2:1034446/1‑150 (MQ=255)
        ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                               <  2:1551047/150‑1 (MQ=255)
         tGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAg                                                                                                                                              <  1:1625610/150‑1 (MQ=255)
         tGACGGCGAAATTTGAAATCACTAAAACGTCGGCAGCGAAGATATCGCCAGAGATTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCAGTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATGTAATCTTTTGTCat                                                                                                                                              >  2:258702/1‑149 (MQ=255)
          gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                             >  2:785753/1‑150 (MQ=255)
          gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                             >  1:152651/1‑150 (MQ=255)
            cGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAAt                                                                                                                                           >  2:162234/1‑150 (MQ=255)
             ggCGAAATTTGAACTCACTAAAACGTCGGCTGGGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                          <  1:309238/150‑1 (MQ=38)
             ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                          <  1:632232/150‑1 (MQ=38)
             ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATACCGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATg                                                                                                                                          >  2:1889746/1‑150 (MQ=255)
              gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGa                                                                                                                                         <  2:601074/150‑1 (MQ=38)
               cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAg                                                                                                                                        >  1:1465339/1‑150 (MQ=255)
               cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGAGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAg                                                                                                                                        <  2:1832220/150‑1 (MQ=38)
                gAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCTCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAgg                                                                                                                                       <  1:909469/150‑1 (MQ=38)
                 aaaTTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGt                                                                                                                                      <  1:2023128/150‑1 (MQ=255)
                  aaTTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGATTGAGGTg                                                                                                                                     >  1:1858481/1‑150 (MQ=255)
                    tttGAAATCACTAAAACGTCGGCTGCGAAGATATAGCCCGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATATAATTTTATCTATCACCTCATTCTGACAAGATTTAATCGTTAGGCAGAATGATGTGaa                                                                                                                                   >  2:1019389/1‑150 (MQ=255)
                          aTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCt                                                                                                                             <  2:1780680/150‑1 (MQ=255)
                          aTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCt                                                                                                                             >  1:54442/1‑150 (MQ=255)
                           tCACTAAAACGTCGGCTGAGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTg                                                                                                                            <  2:1021991/150‑1 (MQ=255)
                            cACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg                                                                                                                           >  2:1095446/1‑150 (MQ=255)
                                aaaaCGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAagg                                                                                                                       >  1:780772/1‑150 (MQ=255)
                                    cGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAggagga                                                                                                                   >  2:1708590/1‑150 (MQ=255)
                                       cGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGCCAAGATTTAATCTTTTGTCAGAAGGAGGTGAATAATCTGGAAGGAGGATTc                                                                                                                >  2:1272552/1‑150 (MQ=255)
                                          cTGAGAAGATATCGCCAGATCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGa                                                                                                             <  2:1071107/150‑1 (MQ=255)
                                           tGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTAGTCAGAATGAGGTGAATAAACTGGAAGTAGGATTCAGaa                                                                                                            >  2:1434735/1‑150 (MQ=255)
                                             cGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                          >  1:226542/1‑150 (MQ=255)
                                             cGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGAGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                          <  1:2038260/150‑1 (MQ=255)
                                                  atatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGTTGAATAATCTGGAAGGAGGATTCAGAAAATTAGc                                                                                                     >  1:596455/1‑150 (MQ=255)
                                                   tatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCg                                                                                                    <  1:1435563/150‑1 (MQ=255)
                                                     tCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGAt                                                                                                  >  1:565767/1‑150 (MQ=255)
                                                      cGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAATTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCCGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGATGATTCAGAAAATTAGCGAta                                                                                                 >  2:406323/1‑149 (MQ=255)
                                                        ccAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCtt                                                                                               >  1:1307521/1‑150 (MQ=255)
                                                         cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                              <  1:1480449/150‑1 (MQ=255)
                                                         cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                              <  1:767887/150‑1 (MQ=255)
                                                                       cTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATccg                                                                                >  1:1596711/1‑150 (MQ=255)
                                                                           aaaGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGt                                                                            >  1:1820304/1‑150 (MQ=255)
                                                                             aGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCa                                                                          >  2:88440/1‑150 (MQ=255)
                                                                                gagaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATg                                                                       >  2:1602567/1‑150 (MQ=255)
                                                                                  gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  2:1891495/150‑1 (MQ=255)
                                                                                  gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  2:219800/150‑1 (MQ=255)
                                                                                  gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  2:1212368/150‑1 (MQ=255)
                                                                                     tGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCg                                                                  >  2:1479682/1‑150 (MQ=255)
                                                                                      gCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGc                                                                 >  1:916782/1‑150 (MQ=255)
                                                                                       cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                                <  2:399371/150‑1 (MQ=255)
                                                                                       cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                                <  1:1034447/150‑1 (MQ=255)
                                                                                       cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                                <  1:1244283/150‑1 (MQ=255)
                                                                                         tCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCaa                                                              >  2:1530837/1‑150 (MQ=255)
                                                                                          cTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGTCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCaaa                                                             >  2:914309/1‑150 (MQ=255)
                                                                                            tAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAAt                                                           >  2:1181477/1‑150 (MQ=255)
                                                                                             aaaTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATg                                                          <  1:436148/150‑1 (MQ=255)
                                                                                               aTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAATATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGAc                                                        <  1:411059/150‑1 (MQ=255)
                                                                                                  actcgcATTTGATTTAATATTATCTATCACCTCATTCTGACAAGATTTAATCTTTTTTCAGAATGAGGTGAATAATCTTGACGGAGGATTCAGAAAATTAGCGATCTTTCCGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCgg                                                     <  2:161884/147‑1 (MQ=255)
                                                                                                  aCGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCgg                                                     <  2:494214/150‑1 (MQ=255)
                                                                                                   cgcgGATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  1:606244/150‑1 (MQ=255)
                                                                                                   cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  1:759748/150‑1 (MQ=255)
                                                                                                   cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    >  2:1630004/1‑150 (MQ=255)
                                                                                                   cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  2:1639765/150‑1 (MQ=255)
                                                                                                   cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  2:1647837/150‑1 (MQ=255)
                                                                                                           tGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATATGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGca                                            >  1:901124/1‑149 (MQ=255)
                                                                                                            gATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCg                                           <  2:1821604/150‑1 (MQ=255)
                                                                                                             aTTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGt                                          >  1:487488/1‑150 (MQ=255)
                                                                                                                tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCTGGCATCCGCCGTCAATGGCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                       <  1:1019390/150‑1 (MQ=255)
                                                                                                                tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                       >  1:681141/1‑150 (MQ=255)
                                                                                                                  aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                     <  2:95815/150‑1 (MQ=255)
                                                                                                                  aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                     >  2:1726699/1‑150 (MQ=255)
                                                                                                                  aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                     >  2:1370511/1‑150 (MQ=255)
                                                                                                                        tctatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTc                               <  2:1401044/150‑1 (MQ=255)
                                                                                                                         ctatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCa                              >  1:440924/1‑150 (MQ=255)
                                                                                                                          tatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTTTAAGGAGGATTCAGAAAATTAGCTATCTTTACGCCACGCATCCTACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTctt                             >  1:1075196/1‑148 (MQ=255)
                                                                                                                          tatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCAt                             <  1:193995/150‑1 (MQ=255)
                                                                                                                          tatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATAAGCGATCTTTACGCCACGCATGCGCCGTCAAAGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCAt                             >  1:1655950/1‑150 (MQ=255)
                                                                                                                            tcACCTCATTCTGACAAGATTTAATCTTTTGTGAGAATGAGGTGAATAATCTGGATGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCATTGCCTCGCCAAAATGACCGTCAATCAGCCGTTTGGTGAGTTCATGc                           <  1:1740949/150‑1 (MQ=255)
                                                                                                                            tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCCCGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                           >  2:529271/1‑150 (MQ=255)
                                                                                                                            tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                           <  1:1488476/150‑1 (MQ=255)
                                                                                                                             cACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCa                          >  1:234693/1‑150 (MQ=255)
                                                                                                                             cACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCa                          <  2:905858/150‑1 (MQ=255)
                                                                                                                                 tCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgcgg                      <  1:1218626/150‑1 (MQ=255)
                                                                                                                                 tCATTCTGACAAAAGATTAAATCTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCAGAAATCAGCCGTTTGGTGAGTTCATCCAgcgg                      >  1:401176/1‑150 (MQ=38)
                                                                                                                                   aTTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgcggcg                    <  1:1889747/150‑1 (MQ=255)
                                                                                                                                    ttCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATACAGCGGCGa                   >  2:727020/1‑150 (MQ=255)
                                                                                                                                       tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc                >  1:1576713/1‑150 (MQ=255)
                                                                                                                                       tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc                <  1:1906192/150‑1 (MQ=255)
                                                                                                                                         aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa              <  2:487488/150‑1 (MQ=255)
                                                                                                                                         aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa              <  2:54442/150‑1 (MQ=255)
                                                                                                                                           aaGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGc            <  2:226542/150‑1 (MQ=255)
                                                                                                                                               tttAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTTAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATAAGCCGTTTGGTGTGTTCATGAAGCGGCGATGCCAGaacaa        >  2:166722/1‑149 (MQ=255)
                                                                                                                                                ttAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCAGCAGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCCTGCAGCGGCTATGACAGCACATc       >  2:1844592/1‑150 (MQ=255)
                                                                                                                                                 tAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATcc      >  2:885230/1‑150 (MQ=255)
                                                                                                                                                   aTCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGAGAGTTCATGCAGCGGCGATGCCAGCACATCcgc    >  1:237715/1‑150 (MQ=255)
                                                                                                                                                   aTCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTGTGGGGAGTTCATGCAGCGGCGATGCCAGCACATCcgc    >  2:1754294/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCCGCGG  >  NC_000913/1351633‑1351931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: