Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,784 T→C 6.5% intergenic (‑24/+44) ycjD ← / ← sapF DUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,7840TC 6.5% 459.2 / 14.0 152intergenic (‑24/+44)ycjD/sapFDUF559 family endonuclease‑related protein/antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (72/70);  new base C (4/6);  total (76/76)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 154 total aligned reads displayed.

TGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCCGCGG  >  NC_000913/1351635‑1351931
                                                                                                                                                     |                                                                                                                                                   
 ggATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCttt                                                                                                                                                    >  1:1391495/1‑150 (MQ=255)
   aTGTTGTCGGCGAAATTTGAAATCACTAACACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGCCAAGATTTAATCTTTTg                                                                                                                                                  <  2:1307610/150‑1 (MQ=255)
   aTGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTg                                                                                                                                                  >  1:1114469/1‑150 (MQ=255)
    tGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGt                                                                                                                                                 >  2:1734400/1‑150 (MQ=255)
    tGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGt                                                                                                                                                 <  1:682061/150‑1 (MQ=255)
      ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTTCAAAGTGCGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                               <  2:2012741/150‑1 (MQ=255)
      ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                               <  2:1750084/150‑1 (MQ=255)
      ttGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCa                                                                                                                                               >  2:1034446/1‑150 (MQ=255)
       tGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAg                                                                                                                                              <  1:1625610/150‑1 (MQ=255)
       tGACGGCGAAATTTGAAATCACTAAAACGTCGGCAGCGAAGATATCGCCAGAGATTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCAGTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATGTAATCTTTTGTCat                                                                                                                                              >  2:258702/1‑149 (MQ=255)
        gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                             >  2:785753/1‑150 (MQ=255)
        gACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                                                                                                                                             >  1:152651/1‑150 (MQ=255)
          cGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAAt                                                                                                                                           >  2:162234/1‑150 (MQ=255)
           ggCGAAATTTGAACTCACTAAAACGTCGGCTGGGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                          <  1:309238/150‑1 (MQ=38)
           ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                          <  1:612419/150‑1 (MQ=38)
           ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATg                                                                                                                                          <  1:632232/150‑1 (MQ=38)
            gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGa                                                                                                                                         <  2:601074/150‑1 (MQ=38)
             cGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGAGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAAAGATTAAATCTTGTCAGAATGAg                                                                                                                                        <  2:1832220/150‑1 (MQ=38)
               aaaTTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGt                                                                                                                                      <  1:2023128/150‑1 (MQ=255)
                aaTTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGATTGAGGTg                                                                                                                                     >  1:1858481/1‑150 (MQ=255)
                      aaaTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGaataat                                                                                                                               >  1:399371/1‑150 (MQ=255)
                        aTCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCt                                                                                                                             <  2:1780680/150‑1 (MQ=255)
                         tCACTAAAACGTCGGCTGAGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTg                                                                                                                            <  2:1021991/150‑1 (MQ=255)
                          cACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg                                                                                                                           >  2:193995/1‑150 (MQ=255)
                          cACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg                                                                                                                           >  2:1095446/1‑150 (MQ=255)
                           aCTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGa                                                                                                                          >  1:23560/1‑150 (MQ=255)
                              aaaaCGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAagg                                                                                                                       >  1:780772/1‑150 (MQ=255)
                                  cGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAggagga                                                                                                                   >  2:1708590/1‑150 (MQ=255)
                                         tGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTAGTCAGAATGAGGTGAATAAACTGGAAGTAGGATTCAGaa                                                                                                            >  2:1434735/1‑150 (MQ=255)
                                           cGAAGATTTCGCCAGAGGTTTCGTTCCTGCATTGTGAGTTTGCGTCTTAAATCACGCGCATTTGATTTAAATTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGaaaa                                                                                                          <  1:187858/150‑1 (MQ=255)
                                               tatatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAg                                                                                                      <  2:1574423/149‑1 (MQ=255)
                                               gATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAg                                                                                                      <  2:1051477/150‑1 (MQ=255)
                                                atatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGTTGAATAATCTGGAAGGAGGATTCAGAAAATTAGc                                                                                                     >  1:596455/1‑150 (MQ=255)
                                                 tatCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCg                                                                                                    <  1:1435563/150‑1 (MQ=255)
                                                   tCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGAt                                                                                                  >  1:565767/1‑150 (MQ=255)
                                                    cGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAATTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCCGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGATGATTCAGAAAATTAGCGAta                                                                                                 >  2:406323/1‑149 (MQ=255)
                                                      ccAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCtt                                                                                               >  1:1307521/1‑150 (MQ=255)
                                                       cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAACTCACGCGCATTTGAGTTAATTTTATCTATCAACTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTCGAATGAGGATTCAGAAAAATAGCGATCttt                                                                                              >  1:178802/1‑150 (MQ=255)
                                                       cAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCttt                                                                                              <  1:1480449/150‑1 (MQ=255)
                                                                cGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGc                                                                                     >  1:419010/1‑150 (MQ=255)
                                                                    ccTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATcc                                                                                 >  1:451036/1‑150 (MQ=255)
                                                                     cTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATccg                                                                                >  1:1596711/1‑150 (MQ=255)
                                                                        cAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATccgccg                                                                             >  2:1888364/1‑150 (MQ=255)
                                                                           aGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCa                                                                          >  2:88440/1‑150 (MQ=255)
                                                                              gagaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATg                                                                       >  2:1602567/1‑150 (MQ=255)
                                                                               agaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCTCCGTCAATGc                                                                      >  1:979693/1‑150 (MQ=255)
                                                                                gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  1:902794/150‑1 (MQ=255)
                                                                                gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  2:1891495/150‑1 (MQ=255)
                                                                                gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  2:219800/150‑1 (MQ=255)
                                                                                gaTTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGcc                                                                     <  2:1212368/150‑1 (MQ=255)
                                                                                   tGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCg                                                                  >  2:1479682/1‑150 (MQ=255)
                                                                                    gCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGc                                                                 >  1:916782/1‑150 (MQ=255)
                                                                                     cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                                <  2:399371/150‑1 (MQ=255)
                                                                                     cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                                <  1:1034447/150‑1 (MQ=255)
                                                                                     cGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGcc                                                                <  1:1244283/150‑1 (MQ=255)
                                                                                      gTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCa                                                               <  1:556926/150‑1 (MQ=255)
                                                                                       tCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCaa                                                              >  2:1530837/1‑150 (MQ=255)
                                                                                        cTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGTCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCaaa                                                             >  2:914309/1‑150 (MQ=255)
                                                                                           aaaTCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATg                                                          <  1:436148/150‑1 (MQ=255)
                                                                                                actcgcATTTGATTTAATATTATCTATCACCTCATTCTGACAAGATTTAATCTTTTTTCAGAATGAGGTGAATAATCTTGACGGAGGATTCAGAAAATTAGCGATCTTTCCGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCgg                                                     <  2:161884/147‑1 (MQ=255)
                                                                                                 cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  2:1647837/150‑1 (MQ=255)
                                                                                                 cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  2:1639765/150‑1 (MQ=255)
                                                                                                 cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  1:1485080/150‑1 (MQ=255)
                                                                                                 cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    >  2:1630004/1‑150 (MQ=255)
                                                                                                 cgcgCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGc                                                    <  1:759748/150‑1 (MQ=255)
                                                                                                      aTTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCa                                               <  2:580939/150‑1 (MQ=255)
                                                                                                         tGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGcc                                            <  1:1919009/150‑1 (MQ=255)
                                                                                                         tGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATATGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGca                                            >  1:901124/1‑149 (MQ=255)
                                                                                                          gATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCg                                           <  2:1821604/150‑1 (MQ=255)
                                                                                                           aTTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGt                                          >  1:487488/1‑150 (MQ=255)
                                                                                                            tttAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTAATAATGAGTTGAATAATATGGAAGGAGGATTCAGAAAATTAGCGATCTTTACTCCACGCATACGCCGTCAATGCCTCGCCAAAATGACCGGCAATCATCCGtt                                         >  1:312460/1‑150 (MQ=255)
                                                                                                              tAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCTGGCATCCGCCGTCAATGGCTCGCCAAAATGACCGGCAATCAGCCGTTTg                                       <  1:1019390/150‑1 (MQ=255)
                                                                                                               aaTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTgg                                      <  2:23560/150‑1 (MQ=255)
                                                                                                                aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                     <  2:95815/150‑1 (MQ=255)
                                                                                                                aTTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGt                                     >  2:1370511/1‑150 (MQ=255)
                                                                                                                 ttttATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTg                                    <  2:504489/150‑1 (MQ=255)
                                                                                                                      tctatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTc                               <  2:1401044/150‑1 (MQ=255)
                                                                                                                        tatcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTTTAAGGAGGATTCAGAAAATTAGCTATCTTTACGCCACGCATCCTACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTctt                             >  1:1075196/1‑148 (MQ=255)
                                                                                                                         atcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATg                            >  1:1438902/1‑150 (MQ=255)
                                                                                                                         atcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGGGAGTTCATg                            <  2:1292594/150‑1 (MQ=255)
                                                                                                                          tcACCTCATTCTGACAAGATTTAATCTTTTGTGAGAATGAGGTGAATAATCTGGATGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCATTGCCTCGCCAAAATGACCGTCAATCAGCCGTTTGGTGAGTTCATGc                           <  1:1740949/150‑1 (MQ=255)
                                                                                                                          tcACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCCCGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGc                           >  2:529271/1‑150 (MQ=255)
                                                                                                                           cACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCa                          <  2:905858/150‑1 (MQ=255)
                                                                                                                           cACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCa                          >  1:234693/1‑150 (MQ=255)
                                                                                                                             ccTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgc                        >  2:1715308/1‑150 (MQ=255)
                                                                                                                               tCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAgcgg                      <  1:1218626/150‑1 (MQ=255)
                                                                                                                               tCATTCTGACAAAAGATTAAATCTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCAGAAATCAGCCGTTTGGTGAGTTCATCCAgcgg                      >  1:401176/1‑150 (MQ=38)
                                                                                                                                  ttCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATACAGCGGCGa                   >  2:727020/1‑150 (MQ=255)
                                                                                                                                     tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc                >  1:1576713/1‑150 (MQ=255)
                                                                                                                                     tGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGc                <  1:1906192/150‑1 (MQ=255)
                                                                                                                                       aCAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCa              <  2:487488/150‑1 (MQ=255)
                                                                                                                                        cAAGATTTAATCTTTTGTCAGAATGAGTTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCGCGCATCCGCCGTCAATGCCTCGCCAAAATGAACGGCAATCAGCCGTTTGGTGAGTTCAAGCAGCGGCGATGCCAg             >  2:1254955/1‑150 (MQ=255)
                                                                                                                                         aaGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGACGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGc            <  2:1117051/150‑1 (MQ=255)
                                                                                                                                         aaGATTTAATCTTTTGGCAGAATGAGGTGAATAATCTGGAAGGAGGAGTCAGAAAATTAGCGATCTTTACGCCACGCAGCCGCCGTCAATGCCTCGCCAAAAAGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCTGc            <  1:2003161/150‑1 (MQ=255)
                                                                                                                                          aGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGca           <  1:780105/150‑1 (MQ=255)
                                                                                                                                             tttAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTTAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATAAGCCGTTTGGTGTGTTCATGAAGCGGCGATGCCAGaacaa        >  2:166722/1‑149 (MQ=255)
                                                                                                                                              ttAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCAGCAGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCCTGCAGCGGCTATGACAGCACATc       >  2:1844592/1‑150 (MQ=255)
                                                                                                                                               tAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATcc      >  2:885230/1‑150 (MQ=255)
                                                                                                                                                  tCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCcgcg   >  1:1628730/1‑150 (MQ=255)
                                                                                                                                                   cTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGAGCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCCGCgg  <  1:2044205/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGGAGGATTCAGAAAATTAGCGATCTTTACGCCACGCATCCGCCGTCAATGCCTCGCCAAAATGACCGGCAATCAGCCGTTTGGTGAGTTCATGCAGCGGCGATGCCAGCACATCCGCGG  >  NC_000913/1351635‑1351931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: