Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,381,915 G→T 5.5% intergenic (+13/‑32) ycjV → / → ompG pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,381,9150GT 5.5% 342.7 / 6.2 115intergenic (+13/‑32)ycjV/ompGpseudogene; putative ATP‑binding component of a transport system/outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base G (64/44);  new base T (4/3);  total (68/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 115 total aligned reads displayed.

ACGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAA  >  NC_000913/1381769‑1382062
                                                                                                                                                  |                                                                                                                                                   
acGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCa                                                                                                                                                  <  2:417405/150‑1 (MQ=255)
  ggTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCAtt                                                                                                                                                <  2:913260/150‑1 (MQ=255)
    ttGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTcc                                                                                                                                              <  1:1669913/150‑1 (MQ=255)
       ggggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTccccc                                                                                                                                           >  2:1768710/1‑150 (MQ=255)
         ggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGATATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAg                                                                                                                                         >  2:240102/1‑150 (MQ=255)
         ggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAg                                                                                                                                         >  1:1254049/1‑150 (MQ=255)
         ggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTAATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACTGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAg                                                                                                                                         >  1:828833/1‑150 (MQ=255)
         ggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATAACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAg                                                                                                                                         >  1:1261715/1‑150 (MQ=255)
         ggCACGAGTTAGTGGTCAGTGCTGGTGCGTTAATTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAATGCCTTccccctg                                                                                                                                         <  1:1564859/150‑3 (MQ=255)
          gCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAATGCCTTccccctgt                                                                                                                                        <  1:613412/150‑4 (MQ=255)
          gCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAgg                                                                                                                                        >  1:2042400/1‑150 (MQ=255)
           cACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGa                                                                                                                                       >  1:160094/1‑150 (MQ=255)
            aCGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGat                                                                                                                                      <  1:2050085/150‑1 (MQ=255)
                gTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataata                                                                                                                                  <  2:913711/150‑1 (MQ=255)
                    gTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAg                                                                                                                              <  1:333334/150‑1 (MQ=255)
                        tCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAAc                                                                                                                          >  2:189524/1‑150 (MQ=255)
                              cTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACaataat                                                                                                                    >  2:1607684/1‑150 (MQ=255)
                                  tGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaa                                                                                                                >  1:1710099/1‑150 (MQ=255)
                                   gCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaa                                                                                                               >  1:1110815/1‑150 (MQ=255)
                                   gCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaa                                                                                                               >  1:416050/1‑150 (MQ=255)
                                    tgTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaaa                                                                                                              <  1:786145/149‑1 (MQ=255)
                                    cGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaaa                                                                                                              <  1:447207/150‑1 (MQ=255)
                                    cGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaaa                                                                                                              >  1:1818910/1‑150 (MQ=255)
                                     gTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaaat                                                                                                             >  1:957673/1‑149 (MQ=255)
                                      ttAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCTCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGt                                                                                                            <  1:1152386/150‑1 (MQ=255)
                                      ttAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGt                                                                                                            >  2:1411895/1‑150 (MQ=255)
                                        aaaTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGtta                                                                                                          <  1:836678/150‑1 (MQ=255)
                                        aaaTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGtta                                                                                                          >  1:34456/1‑150 (MQ=255)
                                         aaTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAGATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGttat                                                                                                         <  1:741657/150‑1 (MQ=255)
                                         aaTGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGttat                                                                                                         >  1:816552/1‑150 (MQ=255)
                                              ttATCATGCAGGAGAAAATATCACTATTCATTTTGAAATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTAccc                                                                                                    <  2:1022291/150‑1 (MQ=255)
                                                aTCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGTGAAGGAATTCCCCCAGGATAAAACAAGGAACAATAATGAAAAAGTTATTAcaagg                                                                                                  >  2:377141/1‑146 (MQ=255)
                                                   aTGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTAc                                                                                               <  2:160094/150‑1 (MQ=255)
                                                    tGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCATGATAATACAAGGAACAATAATGAAACAGTTATTACCCTGTAcc                                                                                              <  1:319447/150‑1 (MQ=255)
                                                        ggAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                          <  1:1982180/150‑1 (MQ=255)
                                                        ggAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                          >  1:455691/1‑150 (MQ=255)
                                                        ggAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGATAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                          >  2:1208442/1‑150 (MQ=255)
                                                        ggAGAAAATATCACTATTCATTTTGATATAACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                          >  2:577935/1‑150 (MQ=255)
                                                          agaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                        <  1:1481276/150‑1 (MQ=255)
                                                           gaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTgg                                                                                       <  1:909759/150‑1 (MQ=255)
                                                                tatCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATTCAGAAACGGAAATAGCAATTCGCTAATTACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtg                                                                                  <  1:1897797/150‑1 (MQ=255)
                                                                 atCACTATTCATTTTGATATGAAGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgt                                                                                 <  1:1325861/150‑1 (MQ=255)
                                                                  tCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgtg                                                                                >  1:2026286/1‑150 (MQ=255)
                                                                  tCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgtg                                                                                <  1:895185/150‑1 (MQ=255)
                                                                       aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                           <  1:1424156/150‑1 (MQ=255)
                                                                        ttCATTTTGATATGACGAAATGTCAGTTCTTTGATTCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGCACAATATTAAAAAAGTTATTACCCTGTACCGAACTGGTGATGTGTGCggg                                                                          >  2:1936836/1‑150 (MQ=255)
                                                                          cATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGaa                                                                        >  1:1284586/1‑150 (MQ=255)
                                                                          cATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGaa                                                                        >  1:1678650/1‑150 (MQ=255)
                                                                            ttttGATTTGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGTAGGCATTGCCCCAGGATTATTCAAGGAACAATAATGTTTTAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATg                                                                      <  1:1420660/150‑1 (MQ=255)
                                                                            ttttGATATGACGATATTTAATTTCTTTGATGCAGAAACGGAAATACCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAAAACAAGGAACAATAATGAAAAAGGTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATg                                                                      <  1:1569725/150‑1 (MQ=255)
                                                                             ttttATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATgg                                                                     <  1:709161/150‑1 (MQ=255)
                                                                              ttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGc                                                                    >  2:496045/1‑150 (MQ=255)
                                                                               tGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGcc                                                                   <  2:1672754/150‑1 (MQ=255)
                                                                                   atGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcg                                                               <  1:563304/150‑1 (MQ=255)
                                                                                   atGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcg                                                               <  2:738273/150‑1 (MQ=255)
                                                                                     gACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAATGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGca                                                             >  2:1816210/1‑150 (MQ=255)
                                                                                       cGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGTGcgca                                                           <  1:1807034/150‑1 (MQ=255)
                                                                                       cGAAATGTCATTTCTTTGATGCAGAAACGGAAAAAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGcaca                                                           <  1:1548143/150‑1 (MQ=255)
                                                                                         aaaTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAgg                                                         <  2:514479/150‑1 (MQ=255)
                                                                                                aTTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGTGCGCAGGccattcc                                                  <  1:1451104/150‑6 (MQ=255)
                                                                                                   tCTTTGATGCAGAAACGGAAATAGCAATTCTCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACATGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggaaag                                               <  2:365421/150‑1 (MQ=255)
                                                                                                    cTTTGCTGCAGTAACTGAACTAGCAATTCGCTAAATACTGGGGGAAGGCATTCCCCCAGCATAATACAAGGACCAATAATGAAAAAGTTATTACCCTGACCCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggaaagg                                              <  2:1326153/150‑1 (MQ=255)
                                                                                                     tttGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggaaagga                                             >  2:800191/1‑150 (MQ=255)
                                                                                                        gATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACg                                          >  2:579869/1‑150 (MQ=255)
                                                                                                           gCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACt                                       <  2:1284585/150‑1 (MQ=255)
                                                                                                            cAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGAACAGGCCGAGGAAAGGAACGACTg                                      >  2:1238194/1‑150 (MQ=255)
                                                                                                            cAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATAACCCTGTACCGCACTGGTGATGTGTGCTGGAATCGCCTGCGCACAGGCCGAGGAAAGGAACGAATg                                      >  2:866743/1‑150 (MQ=255)
                                                                                                             aGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTgg                                     >  1:472276/1‑150 (MQ=255)
                                                                                                             aGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTgg                                     >  1:1838418/1‑150 (MQ=255)
                                                                                                             aGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGAGCACCGGCCGAGGAGACGACCGACTgc                                     >  1:259407/1‑149 (MQ=255)
                                                                                                              gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAATGCATTCCCACAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCATGTACCGCACTGGTGATGTGTGAGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGc                                    >  1:1441144/1‑150 (MQ=255)
                                                                                                              gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGc                                    >  1:360742/1‑150 (MQ=255)
                                                                                                              gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGc                                    >  2:1465940/1‑150 (MQ=255)
                                                                                                                aaCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCAc                                  >  2:10936/1‑150 (MQ=255)
                                                                                                                 aCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACt                                 <  1:1768711/150‑1 (MQ=255)
                                                                                                                    gAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCTAGGAAAGGACCGACTGGCACTTTa                              >  2:1263734/1‑150 (MQ=255)
                                                                                                                    gAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAATGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGAATGGCACTTTa                              >  2:129746/1‑150 (MQ=255)
                                                                                                                       attgCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAata                           <  1:1556233/147‑1 (MQ=255)
                                                                                                                        tAGCAAATCGCAAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGAAATTACCCTGTACCGCACAGGTCATGTGTCAGGGAATGGCCAGCGCACAGCCCGAGGAAAGGAACTACTGGCACTTTAataa                          >  1:3808/1‑149 (MQ=255)
                                                                                                                           cAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCgg                       <  2:916776/150‑1 (MQ=255)
                                                                                                                            aaTTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGgc                      <  2:38331/150‑1 (MQ=255)
                                                                                                                             aTTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGgcg                     >  2:697845/1‑150 (MQ=255)
                                                                                                                               tCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGcgcg                   <  2:416760/150‑1 (MQ=255)
                                                                                                                                cGCTAAATACAGGGGGAAGGCATTCCCCCAGGATATTACAAGGAACAATAATGAAAAAGTTATTAACCTGTACCGCACTGGTGATGTGTGCGGGACTGGCCTGCGGACAGGCCGAGGAACGGACCGACTGGCACTTTAATATCGGcgcgc                  >  1:1042139/1‑149 (MQ=255)
                                                                                                                                cGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAAGACAAGGAACAATAATGAAAAAGTAATTACCCTGTACCGAACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGtcgcca                  >  2:1020273/1‑148 (MQ=255)
                                                                                                                                  cTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATg                >  1:1983801/1‑150 (MQ=255)
                                                                                                                                     aaTACATGGTGAAGGCATTCACCCAGTATAATACAATTAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTTATGTGTGCGGGAATTGCCTGCGCACAGGCCGAGTAAAGTAACGACTTGCACTTTAATATCGGCGCGATGTAc             <  1:158485/150‑1 (MQ=255)
                                                                                                                                       tACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGa           >  1:839180/1‑150 (MQ=255)
                                                                                                                                        cctgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa          >  1:947684/4‑150 (MQ=255)
                                                                                                                                        aCAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa          >  2:151433/1‑150 (MQ=255)
                                                                                                                                        aCAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa          >  2:357533/1‑150 (MQ=255)
                                                                                                                                         ctgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGACCGACTGGCACTTTAATATCGGCGCGATGTACGaaa         >  2:1617670/3‑150 (MQ=255)
                                                                                                                                         cAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaaa         <  2:1261714/150‑1 (MQ=255)
                                                                                                                                         cAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaaa         >  1:1078390/1‑150 (MQ=255)
                                                                                                                                         cAGGGGGAAGGCATTCCCAGAGTATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGAACTGGTGATGTGTGCGTGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaaa         <  2:828832/150‑1 (MQ=255)
                                                                                                                                          tgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAAt        >  2:1044778/2‑150 (MQ=255)
                                                                                                                                           gggggAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATa       >  2:659790/1‑150 (MQ=255)
                                                                                                                                             gggAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAATAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGACCGCCTGGCACTTTATTATCGGCGGGATGTCCGAAATTGa     >  2:1290111/1‑150 (MQ=255)
                                                                                                                                               gAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGATATAGaaa   >  1:1463608/1‑150 (MQ=255)
                                                                                                                                                aaGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaaa  >  2:1949719/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAA  >  NC_000913/1381769‑1382062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: