BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	129,802	T→G	8.7%	K487Q (AAG→CAG)	yacH ←	DUF3300 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	129,802	0	T	G	8.7%	309.2 / 2.6	134	K487Q (AAG→CAG)	yacH	DUF3300 family protein
Reads supporting (aligned to +/- strand): ref base T (62/56); new base G (6/9); total (68/66)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCATAGTGTT  >  NC_000913/129655‑129948

gTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCg                                                                                                                                                  <  1:565608/150‑1 (MQ=255)

  gATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCggg                                                                                                                                                <  1:1725947/150‑1 (MQ=255)

   aTGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTGACGCGCTGCGTCTCTTAGCTGCTGGGTCTTGGGGt                                                                                                                                               >  1:1291510/1‑150 (MQ=18)

       ggACTGAATACGCTCACGAGCAGCGTCTCTTCGCTGCTGGATCTGCGGGTTAGTCTGCATTATCTAGCGAACAGCCTGGCTCTGCTCAGGAGAGGCGGACTGAAAGCGCTCACGCGCTGCCTCTCTTAGATGCTGGATCTTAGGttttct                                                                                                                                           >  2:1171772/1‑147 (MQ=255)

          cTGATTACGCTGTCGCGCTGTGTCTCTTGGCTGCTGGTTCTGCGGGTTAGGCTGTATTTTTTCGCGAACCGCCTGGCGCTGCTCTGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTg                                                                                                                                        <  1:1006843/150‑1 (MQ=255)

            gAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTAGATGCTGGATCTTCGGGTTAGTCTgaa                                                                                                                                      >  2:468481/1‑148 (MQ=255)

               tACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATtc                                                                                                                                   <  1:2009287/150‑1 (MQ=255)

               tACGCTCACGCGCTGCGTCTCTTCGCTGCTGGATCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCAGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATtc                                                                                                                                   >  1:1945360/1‑150 (MQ=255)

                 cGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATtctc                                                                                                                                 >  2:611843/1‑150 (MQ=255)

                 cGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATtcta                                                                                                                                 >  2:1919430/1‑149 (MQ=255)

                 cGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCACTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATtctc                                                                                                                                 >  1:897659/1‑150 (MQ=255)

                    tcgcgcgcgGCGTCTCTTCGCTGCTGGTTCTGTGGGATAGTCTGCATTTTATCGCTAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTcg                                                                                                                              <  2:1411668/147‑1 (MQ=37)

                     gtctggcTTCGTCTCTTGGCTGTTGTTTCTGGGGTGTATTCTGCATTTTCTCGTTAACCTCCTGGCGCTGCTCTGGCGATGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTcgc                                                                                                                             <  1:163427/145‑1 (MQ=255)

                       cgcgCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTAGGGTTAGTCTGCATTCTCTCGCGa                                                                                                                           >  2:1948283/1‑150 (MQ=255)

                         ggcTTCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAAc                                                                                                                         <  1:1630546/149‑1 (MQ=255)

                         cgcTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAAc                                                                                                                         <  1:909202/150‑1 (MQ=255)

                         cgcTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCACTTCGCTGCTGGATCTGAGGGTTACTCAGCATTCTCTCGCGAAc                                                                                                                         >  1:257422/1‑150 (MQ=25)

                             gctgctctTCGCTGCTGGTTCTGCGGGGGAGGCTGCAGTTTCTCGCGAACCGCCTGGCGCTGCTCTGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGcc                                                                                                                     <  2:1099105/146‑1 (MQ=255)

                             gCGTCTCTTCTCTGCTTGTTCTGCGTGTTTTTCTGCATTTTCTCGCTAAACTCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGcc                                                                                                                     <  2:554133/150‑1 (MQ=38)

                             gCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGATTGCGCTGACGAGCTGCCTCTCTTAGCTGCTGGCTCTTCGGGTTAGTCTGCATTATCTCGCGAACTGcc                                                                                                                     >  1:529184/1‑150 (MQ=25)

                              cGTCTCATCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTCGCGCTGCTCAGGCGAGGCGGACTGATTGCGCTCCCGCGCTGCCTCTCTTAGCTGCTGGTTCGTAGGGTTACTCAGCATTCTCTCGAGAACAGCCt                                                                                                                    >  1:223041/1‑150 (MQ=21)

                                 ctctTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGgc                                                                                                                 >  2:668194/1‑150 (MQ=255)

                                   ctTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCGCTTCGGTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGgcgc                                                                                                               >  1:466996/1‑150 (MQ=255)

                                   ctTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACAGCCTGGCGCTGCTCAGGCGAGGCGGACTGATTGCGCTCACGCGCTGCCTCTCTTCGGTGCTGGGACTTCGGTTTACTCTGCATGATCTCGAGGAATGCCtctccc                                                                                                               >  2:341302/1‑145 (MQ=18)

                                       gctgctGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCgctgct                                                                                                           <  2:2044946/150‑1 (MQ=255)

                                       gctgctGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCgctgct                                                                                                           >  1:944291/1‑150 (MQ=255)

                                       gctgctGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGAgctgca                                                                                                           >  1:150689/1‑149 (MQ=255)

                                       gcagctgGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCgctgct                                                                                                           >  1:944293/4‑150 (MQ=255)

                                        gtgttgtttCTGGGGGTTTGTCTGCATGTTCTCTAGAAACGCCTGTCGCGGCTCTGGAGAGGCGGACTGAATTCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCTAACTGCCTGGCGCTGCTc                                                                                                          <  1:1298879/143‑1 (MQ=255)

                                          gctgGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCAGCTCag                                                                                                        >  2:1493857/1‑150 (MQ=255)

                                               ttCTGCGGGTGAGGCTGCAGTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCaggcgag                                                                                                   <  1:1531249/150‑1 (MQ=255)

                                                tCTGCGGGGTAGTCTGCATTTTCTCTCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCaggcgagg                                                                                                  <  1:871168/150‑1 (MQ=255)

                                                 cTGGGGGTTAGTCTGCATTTTCTCGCGAAACTCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGAGCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCaggcgaggc                                                                                                 <  1:102818/150‑1 (MQ=255)

                                                 cTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATTCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCACTCGCGAACTGCCTGGCGCTGCTCaggcgaggc                                                                                                 >  1:1392530/1‑150 (MQ=255)

                                                 cTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTATCGCGAACTGCCTGGCGCTGCTCaggcgaggc                                                                                                 >  2:1132195/1‑150 (MQ=255)

                                                   gCGGGGTAGTCTGCCTTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATCCGCGCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCgg                                                                                               <  1:982358/150‑1 (MQ=255)

                                                      ggctAGTCTGCATTTTCTCCCGAACCCCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTCCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACt                                                                                            <  1:1021282/147‑1 (MQ=255)

                                                          tggcTGCACTTTCTCGCGAACCTCCTGGCGCTGCTCAGGCGAGGCGGACTGAATTCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTGCGGGTTAGGCTGCATTCTCTCGCTAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATa                                                                                        <  1:810943/147‑1 (MQ=17)

                                                          tgTCTGTATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATTCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATa                                                                                        <  1:867180/149‑1 (MQ=255)

                                                            tCTGCTTTTTCTCCCTAAACCCCTGGCGCTGCTCAGGCGATGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGTTTCTTCGGGTTAGGCTGCATTCTCTCGATAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAAc                                                                                      <  2:1223498/150‑1 (MQ=14)

                                                            tCTGCATTTTCTCGCGAACCGCCTGGCGCTGCGCAGGCGCGGATGAGTGAGTGATATGCCGCGCTCACAATCTACGCTGCACGAACGTCGGGTTAGTCGGCTTTCACTCGAGATCTTCCTGCATCTGCTCAGCCTAGGCGGACTGATaaa                                                                                      >  2:262701/1‑149 (MQ=255)

                                                             cTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACg                                                                                     >  2:1560791/1‑150 (MQ=255)

                                                              tGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCCGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGc                                                                                    >  2:222711/1‑150 (MQ=255)

                                                               gCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCt                                                                                   >  2:1290842/1‑150 (MQ=255)

                                                                   tttCTCGCGAACCTCCTGGCGCTGCTCTGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACg                                                                               <  2:1678303/150‑1 (MQ=255)

                                                                   tttCTCCCGAACCTCCTGGCGCTGCTCAGGCGAGGCGGACTGAATTCGCTCACGCGCTGCATCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTATCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACg                                                                               <  1:1335790/150‑1 (MQ=18)

                                                                      ctcGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTGCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAgc                                                                            >  1:1529044/1‑150 (MQ=255)

                                                                       tcGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTTCCTGGCGCTGCTCAGGCGAGGCGGCCTGATAACGCTGAcgcgcg                                                                           >  1:1267591/1‑150 (MQ=255)

                                                                        cgcgAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAgcgg                                                                          >  1:531601/1‑150 (MQ=255)

                                                                        cgcgAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAgcgg                                                                          >  1:1808099/1‑150 (MQ=255)

                                                                        cgcgAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTAGCGAACTGCCTGGCGCTGCTCAGGCGATGCGGACTGATAACGCTGACGAgcgg                                                                          >  2:1442722/1‑150 (MQ=255)

                                                                          cgAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAgcggcg                                                                        <  2:944290/150‑1 (MQ=255)

                                                                          cgAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAgcggcg                                                                        <  2:944292/150‑1 (MQ=255)

                                                                              ccGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGATCTGCCTGGCGCTGCTCAGGCGAGGCGGCCTGATAACGCTGACGAGCGGCGTCCt                                                                    >  1:411406/1‑150 (MQ=255)

                                                                               cGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCtt                                                                   <  1:1919431/150‑1 (MQ=255)

                                                                                tgcgggCGCTGCTCTGGTGTTGCTGATTGAATTCGCTGTCGCGCTTCATATCTTCGCTGCTGTATCTTCGGGTGAGTCTGCATTCTCTCGCGAACATCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCttt                                                                  <  2:330667/146‑1 (MQ=17)

                                                                                gcgtgtcgcTGCGCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTACGGGGTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACTCTGACGAGCGGCGTCCttt                                                                  <  2:1002082/144‑1 (MQ=18)

                                                                                cccTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCttt                                                                  <  1:480926/149‑1 (MQ=255)

                                                                                  cTGGCGCTGCTCAGGCGATGCGGACTGAATCCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCTAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTttg                                                                <  2:819076/150‑1 (MQ=255)

                                                                                  cTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCGTCTCTTCGCTGCTGGCTCTGCGGGTGAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGGGAGGCGGCGTGATAACGCTGCGGAGCGGCGTCCtgtgg                                                                >  1:80999/1‑146 (MQ=17)

                                                                                   tGGCGCTGATCAGGGGATGCGGACTGAAGTCGCTCTCGCGCGGACTCTCTTCGCTGCTGCTTCTGCGGGGGAGTCTGCATTTTCTCGCTAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTttgt                                                               <  2:390471/150‑1 (MQ=17)

                                                                                      cgcTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGCATCTGCGGGTTAGTCTGCATTGTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGc                                                            <  2:516221/150‑1 (MQ=255)

                                                                                       gctgctCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCt                                                           >  2:1584852/1‑150 (MQ=255)

                                                                                       gctgctCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGGCTGCATTCTCTCGCGAACTGCCTGGCGCTGCACAGGCGCGGCGGCGTGATAACGCTGACGAGCGGCGGCCTTTTGTTGCt                                                           >  2:314664/1‑150 (MQ=255)

                                                                                        gtgctCAGGCGATGCGGACTGAATGCGCTCCCGCGCTGCCTCTCTTGGCTGGTGTTTCTGCGGGTTCGGCTGCTTTTTCTCGAGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTg                                                          <  1:250710/149‑1 (MQ=18)

                                                                                        ctgctCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGGCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTg                                                          <  1:1748864/150‑1 (MQ=255)

                                                                                          gctCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGCCGAGCGGCGTCCTGTTGTTGCTggg                                                        >  1:570710/1‑150 (MQ=255)

                                                                                          gctCAGGCGAGGCGGACTGAATCCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCTAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTggg                                                        <  2:618799/150‑1 (MQ=255)

                                                                                           ctCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCAGCTGGATCTTCGGGTTAGTCTGCAATCTCTCGCGAACTGCCTGTCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTgggg                                                       >  1:209951/1‑149 (MQ=255)

                                                                                             cAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCa                                                     >  2:76416/1‑150 (MQ=255)

                                                                                             cAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCGGCGGGTTAGTCTGCATTCTCTCGCTAACTGCCTGGCGCTGCTCAGGCGGGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTggggaa                                                     >  1:334896/1‑147 (MQ=255)

                                                                                             cAGGCGAGGCGGAATGAATGCGCTCACGCGCTGCCTCTCTTCGGTGCTGGATCTTCGGGTTTGTCTGCCTTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGCCTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCGGGGTcc                                                     >  1:36222/1‑149 (MQ=25)

                                                                                                  gaggcgGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGt                                                >  1:1336965/1‑150 (MQ=255)

                                                                                                    tgcgGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAGCGCTGACGAGCGGCGTCGGTTGGGTGCTGGGTCAGTGGGTg                                              >  1:158673/2‑150 (MQ=11)

                                                                                                    ggcgGACTGAATGCGCTGACGCGCTGCCTCTCTTCGCTGCTGGATCTGAGGGTTAGGCTGCATTCTATCGCTAACGGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTg                                              <  1:400216/150‑1 (MQ=21)

                                                                                                       ggACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTATGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGCGTCAGTGGTTGGCg                                           >  2:458989/1‑150 (MQ=255)

                                                                                                         aCTTAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGAc                                         <  2:624466/150‑1 (MQ=255)

                                                                                                            gAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGc                                      >  2:1524743/1‑150 (MQ=255)

                                                                                                             aaTGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGGTGGCGACGGCt                                     >  2:1409009/1‑150 (MQ=255)

                                                                                                                 cgcTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAg                                 <  1:964839/150‑1 (MQ=255)

                                                                                                                 cgcTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGAGAGGCGGACTGATCAGGATTCCGAGCCGCGTCCTTGTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAg                                 >  1:83110/1‑150 (MQ=255)

                                                                                                                  gcTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGt                                <  1:611843/150‑1 (MQ=255)

                                                                                                                    tCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCg                              <  1:1493858/150‑1 (MQ=255)

                                                                                                                      aCGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTg                            >  2:862501/1‑150 (MQ=255)

                                                                                                                        gcgcTGCGTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGa                          <  2:158673/150‑1 (MQ=255)

                                                                                                                         cgcTGCCTCTCTTCTCTGTTTTTGCTTCGGGGTAGCATGCATTTTATCGATAAACTCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGaa                         <  2:1419863/150‑1 (MQ=25)

                                                                                                                              ccTCTCTTCGCTGCTGGCTCATCGGGTTCGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAgtcgt                    <  1:756585/150‑1 (MQ=255)

                                                                                                                                 ctctTCGCTGCTGGATCTTCGGGTTCGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCAt                 <  2:1975360/150‑1 (MQ=255)

                                                                                                                                  tctTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATa                <  2:1671018/150‑1 (MQ=255)

                                                                                                                                   ctTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAg               >  1:1743566/1‑150 (MQ=255)

                                                                                                                                   ctTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAg               >  2:725094/1‑150 (MQ=255)

                                                                                                                                       gctgctGGATCTTCGGGTTAGTCTGCATTCTCTCGAGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGGTGGGTCAGTGGTTGTCGCCGGCTGAAGTCGTGTAAGTCGTCATAGCTcc           >  2:1692068/1‑150 (MQ=37)

                                                                                                                                        ctgctgTTTCTGCGGGCTAGTCTGCATTCTATCCCGAACTTCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTccc          <  2:52021/150‑1 (MQ=38)

                                                                                                                                        ctgctgGATCTTCGGGCCAGGCTGCATTCTCTCGCGAACAGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTccc          <  1:1975137/150‑1 (MQ=255)

                                                                                                                                         tgctgGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCa         <  2:897658/150‑1 (MQ=255)

                                                                                                                                         tgctgGATCTGCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCa         <  2:1291509/150‑1 (MQ=255)

                                                                                                                                          gctgCATCTTCGGGTTCGGCTGCAGTCTCGCGCGAACCGCCTGCCGCTGCGCTGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCAt        <  1:385068/150‑1 (MQ=255)

                                                                                                                                             ggATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGCAGTCGTGGAAGTCGTCATAGCTCCCATAgt     >  1:146197/1‑150 (MQ=255)

                                                                                                                                             ggATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCATAgt     >  1:939720/1‑150 (MQ=255)

                                                                                                                                               aTCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCATAgtgt   >  1:1470279/1‑150 (MQ=255)

GTGATGCGGACTGAATACGCTCACGCGCTGCGTCTCTTCGCTGCTGGTTCTGCGGGTTAGTCTGCATTTTCTCGCGAACCGCCTGGCGCTGCTCAGGCGAGGCGGACTGAATGCGCTCACGCGCTGCCTCTCTTCGCTGCTGGATCTTCGGGTTAGTCTGCATTCTCTCGCGAACTGCCTGGCGCTGCTCAGGCGAGGCGGACTGATAACGCTGACGAGCGGCGTCCTTTTGTTGCTGGGTCAGTGGTTGGCGACGGCTGAAGTCGTGGAAGTCGTCATAGCTCCCATAGTGTT  >  NC_000913/129655‑129948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`