Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,833 G→C 6.1% intergenic (‑90/+21) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8330GC 6.1% 387.4 / 13.7 127intergenic (‑90/+21)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (56/62);  new base C (5/3);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 127 total aligned reads displayed.

TACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTG  >  NC_000913/1441690‑1441980
                                                                                                                                               |                                                                                                                                                    
 acacATCCAGGCATCAGCAGGCTTAGCGCATCAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                                                               <  1:1068502/150‑1 (MQ=255)
  cacaTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                                                                              <  1:1093260/150‑1 (MQ=255)
  cacaTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                                                                              <  2:1685488/150‑1 (MQ=255)
    caTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCTATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCTTTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                                                                            <  2:324732/150‑1 (MQ=255)
     aTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAggggc                                                                                                                                           >  2:693845/1‑149 (MQ=255)
       cccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGc                                                                                                                                         >  1:1169862/1‑150 (MQ=255)
       cccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                                                                         <  1:993557/150‑1 (MQ=255)
        ccGCCATCAGCATGCATAGCGAAACAAACGCGGATACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCAATGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGct                                                                                                                                        <  1:698169/150‑2 (MQ=255)
           ccATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCa                                                                                                                                     <  2:105370/150‑1 (MQ=255)
           ccAGCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCa                                                                                                                                     <  2:1437607/150‑1 (MQ=255)
              tCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                                                                  <  1:1379749/150‑1 (MQ=255)
                agcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTTGGTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGAGGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                                <  1:1662686/150‑1 (MQ=255)
                agcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                                <  1:498721/150‑1 (MQ=255)
                agcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                                <  2:1142024/150‑1 (MQ=255)
                agcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGAGTCAAGAtt                                                                                                                                >  1:284386/1‑150 (MQ=255)
                    ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                                                                            <  1:1994079/150‑1 (MQ=255)
                     gCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                                                                           >  1:1759484/1‑150 (MQ=255)
                                     gcgcCTACTTTCTTCATTGTGGTACTCAATTACAGTTTCTGACTCAGGACTATATTAAGAATAGAGGATGAAAGCTCAATGGGGATTATCTGAATCAGATCCCCTGGAATGCAGGGGAGAAGCTAGATTTAACCAGTTCGTTCGGGAAgg                                                                                                           >  2:1560927/1‑150 (MQ=255)
                                       ggCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGtt                                                                                                         <  1:287035/150‑1 (MQ=255)
                                       ggCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGtt                                                                                                         >  2:159815/1‑150 (MQ=255)
                                         cTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTc                                                                                                       <  1:1651971/150‑1 (MQ=255)
                                          tACTTTCTTCATTGTGGTTCTCAATTACAGGTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCg                                                                                                      >  1:811158/1‑150 (MQ=255)
                                            cTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                                                                    >  2:493127/1‑150 (MQ=255)
                                            cTTTCTTCATTGTGGGTCTCAATTACAGTTTCTGACTCCGGACTATTTTAAGAATAGAGGATGAAACGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                                                                    <  2:465302/150‑1 (MQ=255)
                                              ttcttcATTGTGGTTCTCAATTACAGTATCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTACCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTAAGTTAGCGCAGGTTTctcatt                                                                                                  >  1:211682/1‑145 (MQ=255)
                                                cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCtttt                                                                                                <  1:1936770/150‑1 (MQ=255)
                                                 ttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttttt                                                                                               >  2:766636/1‑150 (MQ=255)
                                                 ttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttttc                                                                                               >  2:857460/1‑149 (MQ=255)
                                                   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTCTcc                                                                                             >  2:675825/1‑150 (MQ=255)
                                                   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTATcc                                                                                             >  2:667147/1‑150 (MQ=255)
                                                    aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCa                                                                                            >  2:754465/1‑150 (MQ=255)
                                                        tgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAtt                                                                                        >  1:68657/1‑150 (MQ=255)
                                                         ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGACTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTg                                                                                       >  2:1101415/1‑150 (MQ=255)
                                                          gTTCCCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGc                                                                                      <  2:1722383/150‑1 (MQ=255)
                                                           ttCTCAATTACAGTTTCTGCCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCt                                                                                     >  1:1213088/1‑150 (MQ=255)
                                                            tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCtt                                                                                    >  2:1876843/1‑150 (MQ=255)
                                                            tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCtt                                                                                    >  1:887673/1‑150 (MQ=255)
                                                             ctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTa                                                                                   >  1:1421283/1‑150 (MQ=255)
                                                               cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAg                                                                                 <  1:414119/150‑1 (MQ=255)
                                                                   tACAGTTTCTGACTCAGGACTATTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                                                                            <  1:1198813/150‑1 (MQ=255)
                                                                   tACAGTTTCGGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                                                                             <  2:1780471/150‑1 (MQ=255)
                                                                     cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                                                                           <  1:578494/150‑1 (MQ=255)
                                                                       gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAg                                                                         >  2:681155/1‑150 (MQ=255)
                                                                         ttCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCg                                                                       <  2:1175575/150‑1 (MQ=255)
                                                                          tCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTTGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                                                                      <  2:708048/150‑1 (MQ=255)
                                                                          tCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAACGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGATTAAGTTTTGCAGCGt                                                                      >  2:643528/1‑150 (MQ=255)
                                                                           cTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTa                                                                     <  1:1168930/150‑1 (MQ=255)
                                                                            tGACTCAGGACTATTTTAAGAATAGAGGATGTAAGGTCATTGTGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCATATTGCTTAAGTTTTGCAGCGTAg                                                                    <  2:1285092/150‑1 (MQ=255)
                                                                              aCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTc                                                                 <  2:1500024/150‑1 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGTGATTATCTGAATCAGCTCCCCTGGAATGCAGGTGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                               <  2:577907/150‑1 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGTATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                               >  2:112323/1‑150 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                               <  2:429598/150‑1 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                               <  2:1053502/150‑1 (MQ=255)
                                                                                 cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTga                                                              >  1:745195/1‑150 (MQ=255)
                                                                                   ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                                                            <  2:1881447/150‑1 (MQ=255)
                                                                                   ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCCGCAAGATTAAACCAGTTCGTTAGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                                                            >  2:376472/1‑150 (MQ=255)
                                                                                      cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt                                                         <  2:1163931/150‑1 (MQ=255)
                                                                                      cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt                                                         <  2:1085889/150‑1 (MQ=255)
                                                                                      atATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt                                                         <  2:1655804/149‑1 (MQ=255)
                                                                                        aTTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTTTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGCCAAGATTAAACCAGTTCGTTCGGGCTGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc                                                       >  2:1392846/1‑150 (MQ=255)
                                                                                         ttttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACt                                                      <  2:1989621/150‑1 (MQ=255)
                                                                                          tttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTACAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTg                                                     >  2:1642302/1‑150 (MQ=255)
                                                                                           ttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAATATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCGGAGAAATACTgg                                                    >  1:190059/1‑150 (MQ=255)
                                                                                              aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCa                                                 <  1:1775734/150‑1 (MQ=255)
                                                                                                aaTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTGCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCaga                                               <  2:1134782/150‑1 (MQ=255)
                                                                                                  tAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGTATGCATTGGAGCGGCAAGTTTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTTAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGc                                             <  1:784608/150‑1 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCt                                            >  1:76193/1‑150 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTATCGCCTGTTTCCCGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTTGTCAGATCt                                            >  2:1124395/1‑150 (MQ=255)
                                                                                                    gagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTAGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCtt                                           >  1:996903/1‑150 (MQ=255)
                                                                                                      ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATTCTGGTCTGAGCTTct                                         >  1:1801613/1‑150 (MQ=255)
                                                                                                       gATGAAAGGTCTTTTGTGATTATGTTAATCAGCTCCCCTGTAATGCAGGGGAGCGGCAAGATTAAACCTGTTCGTTCGTGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctg                                        <  1:401410/150‑1 (MQ=255)
                                                                                                        aTGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgc                                       <  2:881743/150‑1 (MQ=255)
                                                                                                         tGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCATATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgct                                      >  2:2040258/1‑150 (MQ=255)
                                                                                                          taaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgctg                                     <  2:1100528/149‑1 (MQ=255)
                                                                                                          gAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgctg                                     <  2:1342928/150‑1 (MQ=255)
                                                                                                            aaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTc                                   <  1:1876844/150‑1 (MQ=255)
                                                                                                                 cATTGGGGATTATCTGAATCAGCTCCCCTGGAATGAAGGGGAGCGGCAAGATTAAAACAGTTCGATCGGGCAGGTTTCGACTTTTTCCAGATTGGTTAAGTTTTGCAGCGTAGACTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGaa                              >  1:1864033/1‑150 (MQ=255)
                                                                                                                     ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGcc                          <  1:1683477/150‑1 (MQ=255)
                                                                                                                     ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAATAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGTTTGCGTAAGTTTTTCAGCGTAGTCTGAGAAATACTGCTCAGAGCTTCTGCTGTCAGGAATGcc                          <  1:1060309/150‑1 (MQ=255)
                                                                                                                           tATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGc                    <  1:1930673/150‑1 (MQ=255)
                                                                                                                            aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGcc                   >  2:1855657/1‑150 (MQ=255)
                                                                                                                             tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGccc                  <  1:673530/150‑1 (MQ=255)
                                                                                                                              cTGAATCAGCTCCCCTGGAATGCAGGGGCGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCg                 >  1:145741/1‑150 (MQ=255)
                                                                                                                              cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCg                 >  2:885211/1‑150 (MQ=255)
                                                                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCgg                <  2:597543/150‑1 (MQ=255)
                                                                                                                                gAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGt               >  2:2006365/1‑150 (MQ=255)
                                                                                                                                cttgccgCTCCCCTGCATTCCAGGGGAGCTGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGt               >  2:1533698/7‑150 (MQ=255)
                                                                                                                                  aTCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGCTCGTGCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTaa             <  1:83705/150‑1 (MQ=255)
                                                                                                                                    cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc           <  1:1648166/150‑1 (MQ=255)
                                                                                                                                     cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACa          >  2:467209/2‑150 (MQ=255)
                                                                                                                                      gCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAACCCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAg         >  1:1702259/1‑150 (MQ=255)
                                                                                                                                      gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGTGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAg         >  1:194908/1‑150 (MQ=255)
                                                                                                                                       cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGc        >  1:1122135/1‑150 (MQ=255)
                                                                                                                                       cTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCACTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGATTAAGTTTTGCAGCGTAGTCTGAGAAATACTGTTCAGAGCATCTACAGTCAGGAATGCCTGGTCACAGCTAAACAGc        >  1:379988/1‑150 (MQ=255)
                                                                                                                                         ccccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAc      <  2:1421282/150‑1 (MQ=255)
                                                                                                                                         ccccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTGCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGTGATATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAGCCGCAc      >  2:767329/1‑150 (MQ=255)
                                                                                                                                         ccccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTAGGGCAGTTTTCGCATTTTTCCAGATTGGTTAAGTTTTCATAATAATACTAATAATTACTGGTAAAAGTTTGTGGTGTAAGGATTGCCTGTTGCCCGTTAAACGGCAc      >  2:292164/1‑150 (MQ=255)
                                                                                                                                          cccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAATGATCAGAGCTAATGCTGTCAGTAATGCCTGGTTCCCTGTAAACAGCACg     >  1:372303/1‑150 (MQ=255)
                                                                                                                                           ccTGGAATGCAGGGGAGCGGCAAGCTTTAACCAGGACGGGCGGCCAGGGTGCGCCTTTTTCCAGCTTGCTTATGTTTTGCAGGGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCGCGGTAAACAGCACGt    <  2:316785/150‑1 (MQ=255)
                                                                                                                                             tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTtg  <  1:159815/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTG  >  NC_000913/1441690‑1441980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: