Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,807 C→G 5.5% G515R (GGT→CGT)  tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,8070CG 5.5% 303.2 / 7.9 104G515R (GGT→CGT) tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (50/47);  new base G (4/2);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 105 total aligned reads displayed.

ATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCC  >  NC_000913/1449661‑1449956
                                                                                                                                                  |                                                                                                                                                     
aTCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGc                                                                                                                                                    >  2:1322190/1‑150 (MQ=255)
 tCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCa                                                                                                                                                   >  2:1498810/1‑150 (MQ=255)
   gAGGCGGAAATTATAAATATGTTGGGGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATc                                                                                                                                                 <  2:1514/150‑1 (MQ=255)
    aGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCg                                                                                                                                                <  2:475077/150‑1 (MQ=255)
     ggCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGa                                                                                                                                               <  2:636770/150‑1 (MQ=255)
      gCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGAt                                                                                                                                              <  1:278514/150‑1 (MQ=255)
       cGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGTATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATg                                                                                                                                             <  2:133193/150‑1 (MQ=255)
             ttATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCAGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATa                                                                                                                                       >  2:1824566/1‑150 (MQ=255)
              tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                      >  2:1775823/1‑150 (MQ=255)
               ataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGt                                                                                                                                     >  1:484601/1‑150 (MQ=255)
                taAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATATATGCCAATAGTg                                                                                                                                    >  2:959290/1‑150 (MQ=255)
                  aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCCTCGATGCCAATAGTGcc                                                                                                                                  >  2:1002094/1‑150 (MQ=255)
                   atatGTTGGTGTGTAGTACCCTCGATATTGTGATCGATAAGCGTCCGGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCg                                                                                                                                 <  2:307811/150‑1 (MQ=255)
                   atatGTTGGTGTGCAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCg                                                                                                                                 <  2:793002/150‑1 (MQ=255)
                    tatGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGt                                                                                                                                <  2:490990/150‑1 (MQ=255)
                         tGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCa                                                                                                                           <  1:1936378/150‑1 (MQ=255)
                          ggTTTGTAGTACCCACGATATTGTGATCGATAAGCGTTCCGTTGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGTCATCGATGCCAATAGTGCCGTTTTCAt                                                                                                                          <  1:1027898/150‑1 (MQ=255)
                          ggTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCAt                                                                                                                          <  2:751726/150‑1 (MQ=255)
                            tgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                        >  2:661371/1‑150 (MQ=255)
                              tgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGaa                                                                                                                      >  2:1567919/1‑150 (MQ=255)
                               gtagtaCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAg                                                                                                                     >  1:1133897/1‑150 (MQ=255)
                               gtagtaCCCACGAGATTGTGATCGATAAGCGTGCCGTAGCGCGGGTCATCTTTCGCCGGCTCATCGTGCATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACAGGCATCGATGCCAATAGTGCCGTTTTCATGGAAg                                                                                                                     <  2:1412297/150‑1 (MQ=255)
                                 agtaCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGAt                                                                                                                   >  2:711849/1‑150 (MQ=255)
                                   taCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATcc                                                                                                                 <  1:1806947/150‑1 (MQ=255)
                                   taCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTGTCGCCGTCTCATCGTGCTTGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGGAGCACCGGCATCGATGCCATTAGTGCCGTTTTCATGGAAGATcc                                                                                                                 <  2:1244544/150‑1 (MQ=255)
                                     cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAg                                                                                                               <  2:1453274/150‑1 (MQ=255)
                                       cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTc                                                                                                             >  2:1859865/1‑150 (MQ=255)
                                       cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTc                                                                                                             >  1:52925/1‑150 (MQ=255)
                                            tatTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTGAGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGAATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCaaaaa                                                                                                        <  1:997110/150‑1 (MQ=255)
                                             tttGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCTTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAAt                                                                                                       <  1:1142348/149‑1 (MQ=255)
                                             atTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAAt                                                                                                       <  1:1908400/150‑1 (MQ=255)
                                              ttGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATg                                                                                                      <  1:810993/150‑1 (MQ=255)
                                              ttGAGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGAATCGAAGCCAATAGTCCCGTTTTCATGGAAGATCCAGTCAAAAATg                                                                                                      >  2:487768/1‑150 (MQ=255)
                                                gtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCGTCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTa                                                                                                    >  1:1610407/1‑150 (MQ=255)
                                                gtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTa                                                                                                    >  1:1986656/1‑150 (MQ=255)
                                                 tgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTat                                                                                                   >  1:1750656/1‑149 (MQ=255)
                                                 tgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAg                                                                                                   <  2:1395894/150‑1 (MQ=255)
                                                  taTCGATAAGCTTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                                  <  2:522583/149‑1 (MQ=255)
                                                   aTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTc                                                                                                 >  2:374797/1‑150 (MQ=255)
                                                   aTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTc                                                                                                 <  1:1322191/150‑1 (MQ=255)
                                                     cGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCAt                                                                                               >  2:1949965/1‑150 (MQ=255)
                                                         aaGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGtt                                                                                           <  2:1924591/150‑1 (MQ=255)
                                                         aaGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGtt                                                                                           <  1:572029/150‑1 (MQ=255)
                                                          aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTa                                                                                          <  1:374797/150‑1 (MQ=255)
                                                           gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAc                                                                                         >  2:460085/1‑150 (MQ=255)
                                                              ggCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCa                                                                                      <  2:1520054/149‑1 (MQ=255)
                                                                    tgcgcGTGTCATCTTTCTCCGTCTCATCGAGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                                <  1:1935924/149‑1 (MQ=255)
                                                                     gcgcGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACt                                                                               <  2:841986/150‑1 (MQ=255)
                                                                        ggtgtAATCTTTAGCCGTCTCATGGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGAt                                                                            <  2:232708/149‑1 (MQ=255)
                                                                        cgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGAt                                                                            <  1:1495882/150‑1 (MQ=255)
                                                                        cgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGAt                                                                            >  1:987390/1‑150 (MQ=255)
                                                                        cgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGAt                                                                            <  1:468170/150‑1 (MQ=255)
                                                                          tgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATcc                                                                          <  1:1106677/150‑1 (MQ=255)
                                                                             cATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgc                                                                       >  2:1894800/1‑150 (MQ=255)
                                                                               tCTTTCGCCGTCTCATCGTGCATGGTTTTGTCTTAAACACCTTTCACCGCTTCGATGCCCTTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTATTCATAGTTACCCACTGTACTGATCCAgcgc                                                                     <  2:1816747/150‑1 (MQ=255)
                                                                                cTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGca                                                                    <  2:2025755/150‑1 (MQ=255)
                                                                                 tttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCAGTTTTAATGGAAGATCAAGTCAAAAATGTATTCATAGTTACCTACTCAACTGAACCAGCCcac                                                                   >  2:1234941/1‑150 (MQ=255)
                                                                                  ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                                  <  1:1859866/150‑1 (MQ=255)
                                                                                     gCCGTCTCTTCGTGGATGGTTTTCGCTTTAACACCTTTCACCTCTTCGATGAGCGTAGCACCGGCTTCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCTAAAATGTAGTTATAGTTACCCACTGTACTGATCCTGCGCTCCACt                                                               <  2:953154/150‑1 (MQ=255)
                                                                                     gCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACt                                                               >  2:183958/1‑150 (MQ=255)
                                                                                      ccGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTa                                                              <  1:1333939/150‑1 (MQ=255)
                                                                                      ccGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTa                                                              >  2:409443/1‑150 (MQ=255)
                                                                                        gTCACATCGTCCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGTCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAAc                                                            <  2:350028/150‑1 (MQ=255)
                                                                                         tctcATCGTGCCTGGTTTTCGCTTTAACACATTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGACAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACGCCAAGTACTGATCCAGCGCACCACTAACt                                                           >  1:200802/1‑150 (MQ=255)
                                                                                              tCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCgg                                                      <  1:1498811/150‑1 (MQ=255)
                                                                                               cGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGc                                                     >  1:1433395/1‑150 (MQ=255)
                                                                                                gTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCg                                                    <  1:1512340/150‑1 (MQ=255)
                                                                                                gTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGACGTTTTCATTGAAGATCCAGTCAAAAATGTAGTCATAGTTAACCACTGTACTGATCCAGCGCACCACTAACTCCCGGCg                                                    >  1:1942926/1‑150 (MQ=255)
                                                                                                   cATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTc                                                 <  1:455835/150‑1 (MQ=255)
                                                                                                   cATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTc                                                 <  2:1108199/150‑1 (MQ=255)
                                                                                                     tGGTTTTCGCTTTAACCCCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCAGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACAAACTCCCGGCGTGCgg                                               >  2:1229774/1‑150 (MQ=255)
                                                                                                     tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTNGNACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAAGGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCgg                                               >  2:1236256/1‑150 (MQ=255)
                                                                                                     tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACAACTAACTCCCGGCGTTCgg                                               >  1:1698337/1‑150 (MQ=255)
                                                                                                       gTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTa                                             <  2:591498/150‑1 (MQ=255)
                                                                                                            cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCCCCACTAACTCCCGGCGTTCGGTACTGAc                                        >  2:74784/1‑150 (MQ=255)
                                                                                                            cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGAGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACGCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTTCTgcc                                        >  1:1478322/1‑148 (MQ=255)
                                                                                                             gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACg                                       >  2:875326/1‑150 (MQ=255)
                                                                                                             gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACg                                       >  1:635757/1‑150 (MQ=255)
                                                                                                             gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACg                                       <  2:918577/150‑1 (MQ=255)
                                                                                                              cTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGt                                      >  2:624468/1‑150 (MQ=255)
                                                                                                                ttAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTg                                    <  1:1437516/150‑1 (MQ=255)
                                                                                                                 tAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTgg                                   >  1:1055420/1‑150 (MQ=255)
                                                                                                                    cacCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCGTGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCt                                >  1:1144713/1‑150 (MQ=255)
                                                                                                                      ccTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGCTGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg                              >  2:2004312/1‑150 (MQ=255)
                                                                                                                      ccTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCCGCGCACCAATAACTCCCGGCCTTCGGTACTGACGTTGGGCTgg                              >  2:1734948/1‑150 (MQ=255)
                                                                                                                       cTTTCACCGCTTCGATGCCCGGAGCACCGGCATCGATGCCAATAGTGCCGGTTTCATGGAAGATCCAGGCAAAAATGTAGTCATAGTTACCCACAGTACTGATCCAGCGCACCCCTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGc                             <  2:327929/150‑1 (MQ=255)
                                                                                                                           cACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCGCCACTACCTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCag                         >  1:17572/1‑149 (MQ=255)
                                                                                                                              ggcatCGATGCCGGTGCTACGGGCATCGAAGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCAACACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTc                      >  1:451389/5‑150 (MQ=255)
                                                                                                                               gcatCGATGCCGGTGCTACGGGCATCGAAGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                     >  2:1481679/4‑150 (MQ=255)
                                                                                                                               gCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                     >  1:720212/1‑150 (MQ=255)
                                                                                                                               gCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                     <  1:487768/150‑1 (MQ=255)
                                                                                                                                catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                    >  2:644885/3‑150 (MQ=255)
                                                                                                                                cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGATACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                    <  1:323602/150‑1 (MQ=255)
                                                                                                                                      tGCCCGTAGCACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCt              <  2:1252648/150‑1 (MQ=255)
                                                                                                                                      tGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCt              >  2:1728181/1‑150 (MQ=255)
                                                                                                                                        cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTta            >  2:57235/1‑150 (MQ=255)
                                                                                                                                              gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTcc      >  1:600325/1‑150 (MQ=255)
                                                                                                                                              gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTcc      <  2:1833449/150‑1 (MQ=255)
                                                                                                                                              gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCAGTTCCTGATGCTTATACTcc      >  1:1278791/1‑150 (MQ=255)
                                                                                                                                                  cGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTccggcc  <  1:1002095/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCC  >  NC_000913/1449661‑1449956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: