Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,336 C→G 11.4% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,3360CG11.4% 226.4 / 16.3 85pseudogene (945/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base C (43/31);  new base G (6/4);  total (50/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTG  >  NC_000913/1466192‑1466484
                                                                                                                                                |                                                                                                                                                      
tatCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGAt                                                                                                                                                   >  1:1243005/1‑150 (MQ=255)
 ttCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTTGCACCGGCACCCAGAtt                                                                                                                                                  <  2:321111/149‑1 (MQ=255)
  tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTa                                                                                                                                                 >  1:906773/1‑150 (MQ=255)
   cACCGGTGACAGCGCGACGGTGGATAACAATGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGCTCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTaa                                                                                                                                                >  2:1571007/1‑150 (MQ=255)
   cACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTaa                                                                                                                                                >  1:1161642/1‑150 (MQ=255)
   cACCGGTGACAGCGCGACGGGGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTaa                                                                                                                                                <  2:1185778/150‑1 (MQ=255)
     ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAATGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                              <  1:2039725/150‑1 (MQ=255)
      cGGTGACAGCTCGACGTTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATgg                                                                                                                                             <  2:1837340/150‑1 (MQ=255)
       ggTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAACCTCAATAACGAAGGCGAGAGCACCATCACCACCGCTGGCACCGGCACCCAGATTAATGGt                                                                                                                                            >  2:874593/1‑150 (MQ=255)
        gTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtg                                                                                                                                           <  1:1626815/150‑1 (MQ=255)
        gTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGACAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtg                                                                                                                                           <  2:2040078/150‑1 (MQ=255)
          gACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgat                                                                                                                                        >  2:1146517/1‑150 (MQ=255)
            cAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgatga                                                                                                                                      >  2:695706/1‑150 (MQ=255)
            cAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCAATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGAACCAGCACCAACGGTGGCACCGGCACCCAGATTAATGGtgatga                                                                                                                                      >  2:87488/1‑150 (MQ=255)
             aGCGCGACGGTGGATAACAAGGTCACCATGACCGTCACCGATCAGGATTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGGTGAc                                                                                                                                     <  1:329877/150‑1 (MQ=255)
             aGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGAc                                                                                                                                     <  2:73362/150‑1 (MQ=255)
              gcgcGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACg                                                                                                                                    >  2:772461/1‑150 (MQ=255)
                gcgACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGcc                                                                                                                                  >  1:1748073/1‑150 (MQ=255)
                          aTAACAAGGGCACCATGACCGTCACCGGTCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca                                                                                                                       <  1:1634790/150‑1 (MQ=255)
                          aTAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAGGGGTATCCAGAGCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca                                                                                                                       <  1:1901554/150‑1 (MQ=255)
                            aaCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGCCGCCACGGCAaacaac                                                                                                                     >  1:1757154/1‑150 (MQ=255)
                            aaCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaac                                                                                                                     >  2:570116/1‑150 (MQ=255)
                                 gggCACCATGACCGTCACCGATCCGGAGTCCATGCGTATCCAGATCGACGGTGACAAGGCCATCGGCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                <  1:335649/150‑1 (MQ=255)
                                   gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCa                                                                                                              <  1:984178/150‑1 (MQ=255)
                                   gCACCATGACCGTCACCGATCCGGAGTCCATGGTTTACACATATAAGGTGACAAGGCCATATTAAATAAAGAAGGCGACCGCACCATCCCCAAAGGTTGCCCCCGCACCCATATTAATGGTGATTCACCCCACGCAAACAAACAACgcaa                                                                                                              >  2:1972634/1‑147 (MQ=12)
                                      ccATGGCCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGACAGCACCATCACCAACTGTGGCACCGTCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCaaaa                                                                                                           <  1:321140/150‑1 (MQ=255)
                                         tGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccc                                                                                                        >  1:1976136/1‑149 (MQ=255)
                                         tGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAacca                                                                                                        >  2:577961/1‑150 (MQ=255)
                                            ccGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCg                                                                                                     <  1:1624479/150‑1 (MQ=255)
                                               tCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTg                                                                                                  >  2:1347632/1‑150 (MQ=255)
                                                cACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCCAACAACAACGGCAAAACCACCGTTGa                                                                                                 >  1:861112/1‑150 (MQ=255)
                                                 aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTgcc                                                                                                >  1:536533/1‑148 (MQ=255)
                                                  ccGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                               <  2:1817879/150‑1 (MQ=255)
                                                  ccGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                               >  1:1453077/1‑150 (MQ=255)
                                                   cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                              >  2:237139/1‑150 (MQ=255)
                                                    gATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                                                             >  1:898920/1‑150 (MQ=255)
                                                    gATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                                                             <  2:91759/150‑1 (MQ=255)
                                                      tCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                           >  2:101963/1‑150 (MQ=255)
                                                       ccGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGCTGGCCCCGGCACCCAGCTTAATGGTGATGACGCCCCGGCCAACAACAACGGCAAAACCACCGATGACGGCAAg                                                                                          >  2:302237/1‑150 (MQ=18)
                                                        cGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                                                                                         <  2:1161641/150‑1 (MQ=255)
                                                           aGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                      >  1:619973/1‑150 (MQ=255)
                                                              ccATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa                                                                                   >  2:1673018/1‑150 (MQ=255)
                                                               cATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                  >  1:774707/1‑150 (MQ=255)
                                                                aTGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                 <  2:1614117/150‑1 (MQ=255)
                                                                   ggTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGt                                                                              >  2:1598683/1‑150 (MQ=255)
                                                                    gTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGTCAAAACCACCGTTGACGGCAAGGATTCCACCggga                                                                             >  1:978736/1‑148 (MQ=255)
                                                                    gTATCAAGATCGACGGTGACAATGCAATCGTAAATAACGAAGGAGATATCACCTTCTCAAAGGGTTGCTCCGTCACCCAGATTAATGGTGATGACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                             <  2:1561018/150‑1 (MQ=17)
                                                                       tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAATGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACgg                                                                          >  2:1080651/1‑150 (MQ=255)
                                                                       tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACgg                                                                          >  1:1887426/1‑150 (MQ=255)
                                                                         cAGATCGACGGTGACAAGGCCACCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                        >  1:659081/1‑150 (MQ=255)
                                                                          aGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaaa                                                                       <  2:18750/150‑1 (MQ=255)
                                                                           gATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAt                                                                      >  2:1993180/1‑150 (MQ=255)
                                                                            aTCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATc                                                                     >  2:1633822/1‑150 (MQ=255)
                                                                             tCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAttg                                                                    <  1:1598684/150‑3 (MQ=255)
                                                                              cGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGTTTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCaa                                                                   >  2:1989893/1‑150 (MQ=255)
                                                                               gACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACg                                                                                                                               <  1:775052/90‑1 (MQ=14)
                                                                               gACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCCCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACg                                                                                                                               >  2:775051/1‑90 (MQ=17)
                                                                                aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACg                                                                 <  2:906772/150‑1 (MQ=255)
                                                                                aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACg                                                                 <  1:1146518/150‑1 (MQ=255)
                                                                                aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACAAACGGTGGCACCGGAACACAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGATGACGGCAAGGATTCCACCTGTACGGAAATaaaag                                                                 >  1:1112339/1‑148 (MQ=17)
                                                                                 cGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACgg                                                                >  1:853788/1‑150 (MQ=255)
                                                                                  ggTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGt                                                               <  2:1453076/150‑1 (MQ=255)
                                                                                    tGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGtaa                                                             >  1:21751/1‑150 (MQ=255)
                                                                                      aCAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCATATTAATGGTGATGACGCCACGGCAAACAACAACTGCAAAACCACCGTTGACGGCAAGGATTCCACCGTTACGGAAATCAACGGtaata                                                           <  1:1250598/150‑1 (MQ=255)
                                                                                        aaGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAAc                                                         <  2:1809167/150‑1 (MQ=255)
                                                                                            ccATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                <  1:2004497/91‑1 (MQ=255)
                                                                                            ccATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                >  2:2004496/1‑91 (MQ=255)
                                                                                              aTCGTCAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGa                                                                                                                                                    <  2:767148/55‑1 (MQ=14)
                                                                                              aTCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGa                                                                                                                                                    >  1:767149/1‑55 (MQ=255)
                                                                                                cGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGAc                                                                                                                                     >  2:731100/1‑67 (MQ=255)
                                                                                                     aTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTc                                            >  2:1331559/1‑150 (MQ=255)
                                                                                                       aaCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAg                                          <  1:1382089/150‑1 (MQ=255)
                                                                                                        aCGAAGGCGAGAGCACCATCACAAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACAACCGTTGACGGCAAGGAATCCACCGGTACGGAAATAAAAGGAAATAACGGAAAAGTTATTCAgg                                         >  1:55651/1‑150 (MQ=14)
                                                                                                             ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGc                                    <  1:695707/150‑1 (MQ=255)
                                                                                                               cGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGCCGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATTACGGAAAAGTTATTCAGGACGGCGa                                  >  2:1074014/1‑150 (MQ=255)
                                                                                                                 agagCACCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                               >  2:1237733/1‑87 (MQ=18)
                                                                                                                 agagCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCTCCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATc                                <  2:898919/150‑1 (MQ=255)
                                                                                                                     cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                            <  2:1243004/150‑1 (MQ=255)
                                                                                                                       ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGt                                                                                                    >  2:1311270/1‑76 (MQ=39)
                                                                                                                       ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGt                                                                                                    <  1:1311271/76‑1 (MQ=39)
                                                                                                                       ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAGCTGGATg                          >  1:1275500/1‑150 (MQ=255)
                                                                                                                          tCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCa                       >  2:496950/1‑150 (MQ=255)
                                                                                                                           cACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAg                      >  2:1571477/1‑150 (MQ=255)
                                                                                                                            aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                          >  1:1755804/1‑81 (MQ=38)
                                                                                                                                aCGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAACCCACCGTTGACGGCAAGGAATCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcg                 <  2:1976135/150‑1 (MQ=255)
                                                                                                                                     ggcaccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt            <  1:570116/150‑1 (MQ=255)
                                                                                                                                       caccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAAACGGAAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa          >  2:676697/1‑150 (MQ=255)
                                                                                                                                        accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                 <  1:1672819/78‑1 (MQ=39)
                                                                                                                                        accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                 >  2:1672818/1‑78 (MQ=39)
                                                                                                                                        accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc         <  1:772462/150‑1 (MQ=255)
                                                                                                                                         ccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAATGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg        <  1:1080652/150‑1 (MQ=255)
                                                                                                                                          cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                               >  1:1121944/1‑46 (MQ=255)
                                                                                                                                          cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                               <  2:1121943/46‑1 (MQ=255)
                                                                                                                                          cggcaccCAGATAAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGGACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg       <  1:101963/150‑1 (MQ=255)
                                                                                                                                            gcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                          >  2:807604/1‑65 (MQ=40)
                                                                                                                                            gcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                          <  1:807605/65‑1 (MQ=40)
                                                                                                                                               cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                      <  2:795272/66‑1 (MQ=39)
                                                                                                                                               cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                      >  1:795273/1‑66 (MQ=39)
                                                                                                                                               cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGAGGCGGTCACGGTATTg  >  2:1374031/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                      
TATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTG  >  NC_000913/1466192‑1466484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: