Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,482,325 G→A R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,482,3250GA99.0% 301.4 / NA 95R346H (CGC→CAC) yheSABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (57/37);  minor base T (1/0);  total (58/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482179‑3482471
                                                                                                                                                  |                                                                                                                                                  
gTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACaa                                                                                                                                                 <  2:575539/150‑1 (MQ=255)
 ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAAt                                                                                                                                                <  1:1414141/150‑1 (MQ=255)
 ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAAt                                                                                                                                                <  2:1300426/150‑1 (MQ=255)
   ccGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATgg                                                                                                                                              >  1:1824067/1‑150 (MQ=255)
      cTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAATAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcgc                                                                                                                                           <  1:536910/150‑1 (MQ=255)
        ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACGATGGCGCgg                                                                                                                                         >  2:1023878/1‑150 (MQ=255)
        ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCgg                                                                                                                                         <  2:778320/150‑1 (MQ=255)
         tAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCTCATTATTCTCTACTCGATTAAACTGAACCTGGTGCGCGGCTCGCGTATTGGGCTGGTAGGCCACAATGGCGCggg                                                                                                                                        >  1:92004/1‑150 (MQ=255)
              tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                   >  2:863636/1‑150 (MQ=255)
               ccGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTTTTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATc                                                                                                                                  <  1:422277/150‑1 (MQ=255)
               ccGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATc                                                                                                                                  <  1:1864631/150‑1 (MQ=255)
                     gcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTATACTGAACCTGGTGCACGGATCTAGTATTGGTCTGTTAGGCCACAATGGCGCTGGTTAATCGACAtt                                                                                                                            >  2:934634/1‑150 (MQ=255)
                      ccGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCTTTATTCTCGACTCGAGTAAACTGCACCTGGTGCCCGGCTCGCGGATTGGTCTGTTAGGCCACAATGGCGCGGGTAATTCGCCATTa                                                                                                                           >  1:1009775/1‑150 (MQ=255)
                      ccGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTa                                                                                                                           >  1:425332/1‑150 (MQ=255)
                        ggAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGAGCGGGTAAATCGACATTAAt                                                                                                                         >  1:1609977/1‑150 (MQ=255)
                         gAACGCCAGCCAAATCCGATACTGAAGATGGAAAAAGTCAGCCCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGACCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                        <  2:491486/150‑1 (MQ=255)
                             gacTGCCACATCCGTTACTGAAGATGTAAAAAGTCACCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGTCGCGGGTAAATCGACATTAATCAAAc                                                                                                                    <  1:76854/148‑1 (MQ=255)
                               cTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTg                                                                                                                  >  1:864703/1‑150 (MQ=255)
                               cTGCCAAATCCGTTACTGAAGATGGAAAAAGTCACCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGGTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTg                                                                                                                  >  1:263761/1‑150 (MQ=255)
                                tGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGt                                                                                                                 >  2:1450222/1‑150 (MQ=255)
                                   cAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTTGTCTGTTAGGCCACAATGGCGCGTGTAAATCGACATTAATCAAACTGTTAg                                                                                                              <  2:1488680/150‑1 (MQ=255)
                                    aaaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGc                                                                                                             >  1:1881491/1‑150 (MQ=255)
                                            tACTGCAGAAGGAAAATGGCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAAc                                                                                                     <  1:168564/150‑1 (MQ=255)
                                              cTGAAGATGGATAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGTTTAAACTGATCCTGGTGCCCGGCTCGCCTATTGGTCTGGTAGGCCACAATGGCGCGGGTAAATCGAGATTAATCAAACTGTTAGCCGGTGAACtt                                                                                                   >  2:149972/1‑150 (MQ=255)
                                                gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgc                                                                                                 >  1:1611617/1‑150 (MQ=255)
                                                gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgc                                                                                                 >  1:1311213/1‑150 (MQ=255)
                                                gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACAGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGACGGTGAACTTgc                                                                                                 >  1:815420/1‑150 (MQ=255)
                                                 aaGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgcg                                                                                                <  2:59041/150‑1 (MQ=255)
                                                   gATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGcc                                                                                              >  1:764106/1‑150 (MQ=255)
                                                     tGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAg                                                                                            >  2:1500529/1‑150 (MQ=255)
                                                     tGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAg                                                                                            >  1:972188/1‑150 (MQ=255)
                                                      ggAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGt                                                                                           >  2:1636840/1‑150 (MQ=255)
                                                      ggAAAAAGTCAGCGCGGGCTATGGCGATCGCAGTATTCTCGACTCGATTAAACTGAGCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGAGATTAATCAATCTGTTAGCCGGTGAACTTGCGcccgg                                                                                           >  2:235137/1‑147 (MQ=255)
                                                       gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCTCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                          >  2:118316/1‑150 (MQ=255)
                                                       gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                          >  2:1393245/1‑150 (MQ=255)
                                                       gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGCTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                          >  1:934494/1‑150 (MQ=255)
                                                          aaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCTTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGc                                                                                       <  1:951032/150‑1 (MQ=255)
                                                          aaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGc                                                                                       >  1:1884802/1‑150 (MQ=255)
                                                          aaaGGCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGc                                                                                       <  1:1019102/150‑1 (MQ=255)
                                                            aGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCgg                                                                                     >  1:409581/1‑150 (MQ=255)
                                                             gTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCTCCAGTCAGCggg                                                                                    >  2:1329499/1‑149 (MQ=255)
                                                                aGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGaa                                                                                 >  2:756326/1‑150 (MQ=255)
                                                                 gcgcGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGaaa                                                                                <  1:1606049/150‑1 (MQ=255)
                                                                      ggCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGt                                                                           <  1:1039406/150‑1 (MQ=255)
                                                                       gCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTTGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGtg                                                                          >  2:980056/1‑149 (MQ=255)
                                                                       gCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGATGAACTTGCGCCAGTCAGCGGTGAAATTGGTc                                                                          >  2:11311/1‑150 (MQ=255)
                                                                         tATGGCGATCGCATTATTCTCGACTCGATTAAACTGACCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTg                                                                        <  2:107356/150‑1 (MQ=255)
                                                                         tATGGCGATCGCAGTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCGCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTg                                                                        <  2:266753/150‑1 (MQ=255)
                                                                               gATCGCATTATTCTCGCCTCGATTAAACTGACCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTAAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                  >  2:1236844/1‑150 (MQ=255)
                                                                                aTCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAg                                                                 <  2:975412/150‑1 (MQ=255)
                                                                                  cGCATTATTCTCGCCTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGAGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAggg                                                               >  2:1434457/1‑150 (MQ=255)
                                                                                   gCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAACCTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGa                                                              >  1:1414901/1‑150 (MQ=255)
                                                                                   gCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCGCGCGTATTCGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACAATAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGa                                                              <  2:132780/150‑1 (MQ=255)
                                                                                    cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                             >  1:1008935/1‑150 (MQ=255)
                                                                                           ctcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTc                                                      <  2:1824067/150‑1 (MQ=255)
                                                                                           ctcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTc                                                      <  2:1703040/150‑1 (MQ=255)
                                                                                             cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    >  2:630175/1‑150 (MQ=255)
                                                                                             cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    >  1:1307513/1‑150 (MQ=255)
                                                                                             cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    >  2:1725386/1‑150 (MQ=255)
                                                                                              gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   <  2:759492/150‑1 (MQ=255)
                                                                                              gactcgaTTAAACTGAACCTGGAGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATCTCGAGGGAAAATCGACATAAATCAAACTGTTAGCCGGTGAACTTGAGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   >  1:77303/1‑150 (MQ=255)
                                                                                                 tcgaTTAAACTGAAGCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAc                                                <  2:876855/150‑1 (MQ=255)
                                                                                                  ccaatAAACAGACCCTGGTCCCCGCCTCGCGTATTGGTCTGAGAGGCCACAATGGCGCGGGTACATCGACAATAATCAAACTGTTATCCGGTCAACTTGCCCCAGTCAGCGGTGAAATTGGTCTGCCGAAAGGGATCAAACTCGGCTACt                                               <  2:92004/146‑1 (MQ=255)
                                                                                                     ttAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCg                                            >  1:1622139/1‑150 (MQ=255)
                                                                                                      tAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGc                                           >  1:964616/1‑150 (MQ=255)
                                                                                                       aaaCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGcc                                          <  2:619705/150‑1 (MQ=255)
                                                                                                        aaCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGccc                                         >  1:1353215/1‑150 (MQ=255)
                                                                                                         aCTGAACCTGGTGCCCGGGTCGCGATATGGTCTGCTATGCCACACTGGCGCGGGTAAAACGACATAAATCACACTGTTCGCCGGTGAACTTGCGCCATTCAGCGGTGAAATAGGGCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCa                                        <  2:1835884/150‑1 (MQ=255)
                                                                                                          cTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAg                                       >  1:1676285/1‑150 (MQ=255)
                                                                                                          cTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAg                                       >  2:422047/1‑150 (MQ=255)
                                                                                                          cTGAACCTGGGGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAg                                       <  1:545311/150‑1 (MQ=255)
                                                                                                           ggAACCTGCTGCCCGGCTCGCGTCTTGGTCTGTTAGGCCACAATGGCGCGGGTACATCGACATTAAGCAACCTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGGGAAATTGGGCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGc                                      <  2:84567/149‑1 (MQ=255)
                                                                                                              aCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATAGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTGGCCCAGCATc                                   >  2:727598/1‑150 (MQ=255)
                                                                                                                cTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCaa                                 >  1:458357/1‑150 (MQ=255)
                                                                                                                     gCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGa                            >  2:756014/1‑150 (MQ=255)
                                                                                                                      cccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGaa                           <  2:524889/150‑1 (MQ=255)
                                                                                                                       ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                          <  2:403378/150‑1 (MQ=255)
                                                                                                                       ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                          >  1:1528391/1‑150 (MQ=255)
                                                                                                                       ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                          >  2:1268261/1‑150 (MQ=255)
                                                                                                                         ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                        <  1:1383014/150‑1 (MQ=255)
                                                                                                                            tCGCGTAATGGTCTGGTACGCCACAATGGCGCGGGTAAAACGACATTAATCAAACTGTTAGCCGGTGACCTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTg                     <  2:1167451/150‑1 (MQ=255)
                                                                                                                                gTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAACCTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTACGCCAACCATCAACTTGAATACATgctcc                 >  2:314340/1‑147 (MQ=255)
                                                                                                                                  aTTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGcc               <  2:677715/150‑1 (MQ=255)
                                                                                                                                     ggTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgac            >  2:1223611/1‑150 (MQ=255)
                                                                                                                                         tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGATTACCTGCGCGCCGACGAAt        >  2:1311638/1‑150 (MQ=255)
                                                                                                                                         tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCAGGTGAACTTGCGCCAGTCAGCGTTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt        >  2:828584/1‑150 (MQ=255)
                                                                                                                                            tAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTTGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAc     >  2:1174277/1‑150 (MQ=255)
                                                                                                                                            tAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAc     >  1:1899113/1‑150 (MQ=255)
                                                                                                                                             aGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAcc    <  2:1331658/150‑1 (MQ=255)
                                                                                                                                             aGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAcc    <  1:716519/150‑1 (MQ=255)
                                                                                                                                             aGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAcc    <  2:1057131/150‑1 (MQ=255)
                                                                                                                                              ggCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTTGCGGTTGAACTTGCGCCAGGCAGCGGTGAATTTGGTCTGGCGAAAGGGAAAAAAGTCGGCTACTTCGCCCAGCTTCAACTGGATTACCTGCGCGCCGCCGAATCAcgt   >  2:978282/1‑148 (MQ=255)
                                                                                                                                              ggCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCt   >  2:395946/1‑150 (MQ=255)
                                                                                                                                              ggCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCt   >  2:1425279/1‑150 (MQ=255)
                                                                                                                                               gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  >  1:790510/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482179‑3482471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑