| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,560,455:1 | +G | 100% | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 94.9% | 105.2 / ‑0.4 | 39 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (14/22); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG > NC_000913/3560313‑3560587 | atCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCGACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑Gc > 2:779613/1‑143 (MQ=255) aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑Gcgcag < 1:424638/143‑5 (MQ=255) gacggCGTCGTGTCATTGTGGATTTGCCCCGCCACTTTGCGGGCCATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGAt < 2:191893/139‑1 (MQ=255) aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATc < 1:625956/143‑1 (MQ=255) gAGCCGTGGGGGATTGGCTCCGGCGCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTg < 2:499849/143‑1 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCCCCGCCATGATA‑GCGCCGGCTCAGGCTTTGccc > 2:470779/1‑141 (MQ=255) cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCACGATG‑GCGCAGGATCAGCTTTCGCTcc > 2:299728/1‑143 (MQ=255) agtGGGGATGGGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCg < 1:398213/141‑1 (MQ=255) aTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTCCCACGCCGTGTTAACCGAACTGCAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCg > 1:241628/1‑143 (MQ=255) gtGGGGATTTGCTCCGCCAGTTTGCCGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGc < 2:304450/142‑1 (MQ=255) ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGGCCGTCCTTCCAAACTG‑CG‑CAGGAGCAGGTTTTGCTCCGCCa > 1:341121/1‑143 (MQ=255) gtgttGCTCCGCCACGTTGCGGGCGGTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCt < 2:156536/140‑1 (MQ=255) gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGGTTTGCGCCGCCAGCTCCTTGAGGTc > 2:501915/1‑143 (MQ=255) cgatttGCGGGCGATGCGCCCTTGTGCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCATCAGTCTTGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc < 2:445876/140‑1 (MQ=255) tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTCgcggc > 2:38631/1‑143 (MQ=255) gCGGGCGATGCGCTCTGTGTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 2:527982/143‑1 (MQ=255) cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt > 2:649254/1‑143 (MQ=255) cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCAACGGCGTGTTCACCGCACTGCAAGGCCGC‑GCCGCACCGCCCTGATC‑GCGCCGGATCAGGATCTGCTCCCCCAGGTCGTCGAGCTAgcggtcaaa > 2:692073/1‑138 (MQ=255) ggggGATGCGCTCGTTTGCTTCGGGCTGGGTGGGCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 2:683103/143‑1 (MQ=255) gggCGTTGCGCTGTTTGTTGTCGGTCTGGGTTGCCTTGCGAGCGTGGCACGGCGTGTTAACCGAGCTGGAAGGCAGC‑GCCGCACCGCCAGGATG‑GCGCAGGATCAGGTTTTGCTCCGCCTGCTCATTGAGGTCGCGGCGAATa < 2:73754/143‑1 (MQ=255) ctaTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAGCTGGCAACCTTC‑CCCCCACCGCCATGTTG‑TCGTTGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc < 1:268538/141‑1 (MQ=255) tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc < 1:530170/143‑1 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTCGTCTGCGGGCt > 1:555221/1‑143 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga > 1:567238/1‑143 (MQ=255) tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgagca > 1:791259/1‑141 (MQ=255) gTCTGGGTGGCCCTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg < 1:361912/143‑1 (MQ=255) gccgCCTTCCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc < 2:607109/140‑1 (MQ=255) tGGCCTGGCGCTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc < 1:276684/143‑1 (MQ=255) tGGCCTGGCGATCGTGCAACGGCGTGTGAAGCGGACTGGAAGGCAGC‑GGCGCACCGCCATGATG‑GCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc < 2:83491/143‑1 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGAAGGATCAGGTTTTGCCCCGCCAGCTCATCGAGGTCGCGGCGAATACTCCGCAGACTGACGGGAAGATGCGCTACCAGct > 2:1713/1‑143 (MQ=255) ccACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATGATA‑AAGGAACAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGACGAATAGTCTGCGGGCTGACGGAGAAATGCTCGACCAGCTCTTCGGTACTgg > 1:347076/1‑142 (MQ=255) taCCGAGCTGGAAGGCAGC‑TCCCCGCCGCCATGATG‑GCGCTGGAGCACGTTTTTCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:748442/142‑1 (MQ=255) aCCGAACTGGTAGGCTGC‑GCCGCACCGCCATGATG‑GCTCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg < 1:330102/143‑1 (MQ=255) cgcccTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt < 1:501915/139‑1 (MQ=255) tGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc > 2:221851/1‑143 (MQ=255) tGGAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc > 1:756586/1‑143 (MQ=255) ggAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa < 2:232944/143‑1 (MQ=255) gAAGGCAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg < 1:495483/143‑1 (MQ=255) cAGC‑GCCGCACCGCCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa < 1:331784/143‑1 (MQ=255) gccccgcACCGCCATGACG‑GCGCAGGATCAGGTTTTGCCCCGGCAGCTCATTGACGCCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata < 2:12756/140‑1 (MQ=255) ccATGATG‑GCGCCGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCGTACTGTTTAAGCAGCACGATCATCCCGTtgta > 2:553509/1‑142 (MQ=255) | ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGC‑GCCGCACCGCCATGATG‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG > NC_000913/3560313‑3560587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |