| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | T→A | 100% | I690N (ATC→AAC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | A | 100.0% | 143.9 / ‑6.7 | 49 | I690N (ATC→AAC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (22/25); minor base C (0/1); total (22/27) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124960‑125220 | tCAGTCGCTGACTATCCCGAACTGTATCACATCCGACCCGGCTGACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGaaa < 1:416190/143‑1 (MQ=255) tCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGACTGGAGCGTACGTACGGCGACAAACAAGCGAACGCTTACTACCCCAACACTACGCTGCACGAACACTa > 2:13321/1‑143 (MQ=255) ggcggACTAGCCCGAACCGCATCTCTGACGGCCCGGCTTACGCGTACGAAGTGGCTGTCATCATGCATGACGGTCTGGAGCGCACGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGACCACTAc < 2:407175/139‑1 (MQ=255) tCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCg < 1:529955/143‑1 (MQ=255) cccGAACTGTATCTCTTACGTCCCTGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCgg < 1:352547/143‑1 (MQ=255) cccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCgg < 2:555266/143‑1 (MQ=255) tATCTCTTACGACCCGGCTGACGCTTACGAAGTTGCTGTCATCGTGCGTGGGTCGCCCAAGCGTATGAACTTTTAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCgg < 2:173175/143‑1 (MQ=255) tctcTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGAGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGaa < 2:567414/143‑1 (MQ=255) ctctTACGACCCGGCTTACGCTTACGAAGTTGCTGGCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGGTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 1:545398/143‑1 (MQ=255) ctcgTACGTCCCGGCTGACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGGGAAAAACAAGAGAACGTTTACTACTACAACACTGCGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 2:392080/143‑1 (MQ=255) ctattgcGACCCGGCTTCCGGTTACGCATTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 2:4224/137‑1 (MQ=255) tctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTCCGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCCATGCCGGAAgg > 1:228211/1‑143 (MQ=255) cGACCCGGCTGACGCGTACGAAGTGGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGTGATCGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTg < 2:541617/143‑1 (MQ=255) gACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa > 1:13567/1‑143 (MQ=255) gACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa > 1:250684/1‑143 (MQ=255) cGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGaa < 1:98884/143‑1 (MQ=255) ggCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 1:721589/143‑1 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa > 1:346780/1‑143 (MQ=255) gcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCg > 1:669463/1‑143 (MQ=255) ttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTa > 1:35191/1‑143 (MQ=255) tacgAAGTTGCTGTCATCATGCATGACGGTCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTac > 2:786172/1‑142 (MQ=255) gAAGTTGCTGTCATTAAGCATGACGGCCTGGAGCGTATGTACGGAGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACTGGCTTGCTATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAgg < 2:208644/143‑1 (MQ=255) ggcatcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa < 2:346516/141‑1 (MQ=255) ggcatcaaGCATGAGAGTCTGGAGCGCACGTACGGAGAAAAACAAGAGAACGTTTACTACTACAACACGACGCTGGACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa < 2:282124/141‑1 (MQ=255) gTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa > 1:808316/1‑143 (MQ=255) tcatcaTGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTCACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaaa > 1:724312/1‑143 (MQ=255) gcgtgACGGTCTGGTGCGGATGGACGGTGAAAAACAAGAGAACGTTTAACACTACAACACTCCGCTGGACGAAAGCTACCACATGCCGGCAATGCCGGAAGGTGCGGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 2:376636/140‑1 (MQ=255) gcatgACGGTCTAGAGCGTATGTACGGTGAAAAACAAGAGCACGTTTACTACTACACCCCTACGCTGAACGAAAACTACCACATGCCGGCAATGCGGGAAGGTGCTGAGGAAGGCATCCGTAAAGGTATCTACAAACTCGaaa < 1:788318/143‑1 (MQ=255) atgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACACACTCGAAACt > 1:622766/1‑143 (MQ=255) tgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCACTGCCGGAAGGGGCTGAGGAAGGAATCCGTACAGGCATCTACAAACTCGAAAcct > 2:99213/1‑141 (MQ=255) aCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTATGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt > 1:454703/1‑143 (MQ=255) cGGTCTGGAGCGTATGTACGGCGAAAAACAAGCGACAGTATACTACTACAACACTACGCTGCGCGCAAGCTACCACATGCCGGCAATGCGGGACGGTAGAGAGGAAGGCATCCACAAGGGTATCTAAAAACCAGAAAcactta > 2:231606/1‑138 (MQ=255) tATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAg < 1:583516/143‑1 (MQ=255) tGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGACGCTGCTGAGGAAGGTATCCGTAACGGTATCTACAAACTCGAAACCAGTGAAGGTAGCCAAggc > 2:162389/1‑142 (MQ=255) gTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACAACCACATGCCGGCAATGCCGGAAGGTGCTGAGGGAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAACGTAGCAAgggga > 2:636202/1‑141 (MQ=255) ctgTGAAAAACAAGCGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAg < 1:773108/141‑1 (MQ=255) gAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCa < 2:35191/143‑1 (MQ=255) aaaaaCCATATAACGTCTAATACTACAACACCCCCCTGAACGAAAACTACCACACGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAg < 1:678970/143‑1 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAg < 1:750131/143‑1 (MQ=255) aaaCAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAGGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGGAAAGTTCAgct > 1:528136/1‑143 (MQ=255) gaACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGGGAGGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACCATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCt > 2:256120/1‑143 (MQ=255) cGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTAGGCTCCg > 1:779771/1‑143 (MQ=255) tttACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCGAAGGTAAAGTTCAGCTGCTCGGCTCCGGt > 1:209645/1‑143 (MQ=255) ttACTACTTCAGCGCTACGCTGAACCAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGCAAGGTAGCCGTAAAGGTATCTACAACCTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt < 2:53077/143‑1 (MQ=255) ttACTACGACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAGCCGTAAAGGTATCTACAAACTCGAAACTAGTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt < 2:593871/143‑1 (MQ=255) tgcctactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt < 2:186729/140‑1 (MQ=255) ctactacAACACTGGGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTa < 2:303985/143‑1 (MQ=255) tactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAt > 1:93917/1‑143 (MQ=255) tactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGGAAAGGTATCTACAAACTCGAAACTATTGACGGTAGCACAGGTAAAGTTCAGCTGCTCGGCTCCGCTTCtct > 2:31122/1‑141 (MQ=255) | TCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124960‑125220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |