| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,311,830 | A→G | 11.0% | intergenic (+22/+18) | tonB → / ← yciA | Ton complex subunit TonB/acyl‑CoA thioesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,311,830 | 0 | A | G | 11.0% | 73.8 / 3.6 | 37 | intergenic (+22/+18) | tonB/yciA | Ton complex subunit TonB/acyl‑CoA thioesterase |
| Reads supporting (aligned to +/- strand): ref base A (18/14); new base G (2/2); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-02 | |||||||||||
ATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAA > NC_000913/1311689‑1311972 | aTGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTAAGTAAGCAGCAAGT‑GAAAAGCCTCCGAc > 2:130600/1‑143 (MQ=255)aTGTTTGAGCGAGAGGTGAAAAATGCGATGCGCAGCTGGCGTTATGAGCCGGGTCAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGCAATTCAGTGAGCAGCAAGG‑CAAACGCCCACGAc > 2:131349/1‑143 (MQ=255) tGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGTAGTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGATATTCAGTCAGCAGAAAGT‑CAAAAGCCTCAGAcagaaa > 1:117587/1‑138 (MQ=255) cGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCtt < 2:477017/143‑1 (MQ=255) tggggTGAAGAAGGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTTTTTAAAATTAACGCCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGGTCGGAGGCtttt < 2:411887/140‑1 (MQ=255) gTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGGTCGGAGGCTTTTGACt < 1:743985/143‑1 (MQ=255) tGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTCAGCAGAAAGT‑CAAAAGCGTCCGACCGGAGGCATTTGACTa > 1:747653/1‑143 (MQ=255) gAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAGAAGAAGGGCACCACCGAGATTCAGTAAGCGGAAAGT‑CAGAGGCCGCCGACCGGAGGCCTGTGGCTAt > 2:669047/1‑143 (MQ=255) aaaaTGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGTGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTa < 1:133705/143‑1 (MQ=255) tgcgatgcgCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCa < 1:657398/143‑1 (MQ=255) gatgcgCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGCCCGGAGGCTTTTGACTACTACTCAACa > 1:83503/1‑143 (MQ=255) gcccAGGTGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGt < 1:211017/140‑1 (MQ=255) tATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGtt > 1:393440/1‑143 (MQ=255) tGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa > 1:29774/1‑143 (MQ=255) ttGTGCTGAATTTCATGTTTAAAGTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACta < 2:746040/143‑2 (MQ=255) tggtgAATACGCGGTCTAAAATTCACGGCACCAGCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTaaa < 2:24400/143‑1 (MQ=255) gtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTCTTGCCTATCACTCAACAGGAAAGGCGCGAGGGTTTCCTCCCGGATCAACCGCGGCGTAcgcgacaa > 2:39399/1‑136 (MQ=255) tatCCTGTTGAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCt < 2:470715/143‑1 (MQ=255) tttAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTATTCCTTCAGGATCAACCGCAACATACTTAAATAATGCTTATGtga > 1:437817/1‑141 (MQ=255) tAAAATTAACGGCACCACCGAAATTAAGAAAG‑AAAAAGTACAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCCCGACATACTTAAATAATGCTTCTGTCGCt > 1:385119/1‑143 (MQ=255) aaaaCTACCGTCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCtt < 1:259240/143‑1 (MQ=255) aaatcgaCGGCCCCACTGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCttt < 2:119018/137‑1 (MQ=255) ttAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTtata < 1:17042/143‑1 (MQ=255) ggCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATACCGtt > 1:358581/1‑143 (MQ=255) gccaccGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTGAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGcc < 1:747776/142‑1 (MQ=255) accGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCaa > 1:83016/1‑143 (MQ=255) accGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCaa > 2:312737/1‑143 (MQ=255) cGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCCAtt > 1:276251/1‑143 (MQ=255) aaaTTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTCCCCGATTgg > 2:112885/1‑143 (MQ=255) gCAGAAAGT‑CAACAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTa < 1:613400/143‑1 (MQ=255) ccgAAAGG‑CGAACGCCTCCGACCGGAGGCTGTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGTTCCACCGCGACATACTTAAATCATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTAc < 1:131349/141‑1 (MQ=255) cAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTAc < 1:130600/143‑1 (MQ=255) aGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGCGGACTTCCTTCAGGCTCAACCGCGACATACTTAAATACTGCTTCTGTCCCCTTGTCGCGCTGCCCGACTCGTCCCGACGCTCCt > 2:515924/1‑143 (MQ=255) t‑CAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACATTTTTc > 1:510855/1‑143 (MQ=255) aaaaGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCAc > 1:155817/1‑143 (MQ=255) tCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACt > 2:429794/1‑143 (MQ=255) tCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAACTGGTTCAGACGCTACTTTTTTCAACCACCCt > 2:431673/1‑143 (MQ=255) aCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCaa < 2:513637/143‑1 (MQ=255) | ATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGT‑CAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAA > NC_000913/1311689‑1311972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |