| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,466,336 | C→G | 8.4% | pseudogene (945/2518 nt) | ydbA → | putative outer membrane protein, N‑terminal fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,466,336 | 0 | C | G | 8.4% | 141.4 / 3.2 | 53 | pseudogene (945/2518 nt) | ydbA | putative outer membrane protein, N‑terminal fragment |
| Reads supporting (aligned to +/- strand): ref base C (20/28); new base G (2/3); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGT > NC_000913/1466194‑1466461 | tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGATGGCGAGAGCACCATCACCAACGGTggcaccggcacc > 1:639869/1‑143 (MQ=255) cACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACAAGGCCATCGTCAATAACGGAGGCGAGAGCACCATCACCCACGGTGGCACCGGCAccc > 1:253609/1‑143 (MQ=255) cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCATCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGa < 1:84879/143‑1 (MQ=255) aCAGCGCGACGGTGGATCACAAGGGCACCACGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAt < 1:804599/143‑1 (MQ=255) cAGGGCGACGGTGGATAAGAAGGGCACCATGACCGTGACCGATGCGGAGGCCATGGGTATCCAGGTCGACGGTGAGAAGGCCATCGTCGATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCGCCCAGATTAATg < 2:372203/143‑1 (MQ=18) agggcgACGGTGGATAACGAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATgg < 2:92648/140‑1 (MQ=255) gagcgACGGTAGCTAACAGGGGCACCATGGCCGTCACCGGTCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAGCGTCAATAACGAACGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGt < 2:415669/141‑1 (MQ=21) gcgAGGGTGGGTGACGAGGGCACCATGACCGTCAGCGATCGGGAGGCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtga < 2:108500/143‑1 (MQ=18) aCGGTGGATAACAAGGGCACCATGGCCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgatga < 1:584131/143‑1 (MQ=255) aCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCa < 1:397507/143‑1 (MQ=255) ggCACCAGCACCCACACCAAACCGGCGTCCATGGGTATCCAGATCGACGCTGACAAGGCCATCGTCAAGAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaa < 1:147232/143‑1 (MQ=18) gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCACTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaac > 1:380439/1‑143 (MQ=255) gaTGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACg < 1:92936/142‑1 (MQ=255) cATGACCGTCACCGACACGGAGTCCAGGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACg < 2:129827/143‑1 (MQ=255) cATGACCGGCACTGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCACTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACg < 1:412783/143‑1 (MQ=255) tgtcggTCGCCGAGCGGGAGTCCGTAGGTAGCTGGGTCGGCGGTGACAAGGCCATCGGCGATAACGAAGGCGACAGCACCAGCAGCAACGGTGGGACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc < 2:234016/138‑1 (MQ=17) tgcccGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGCGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc < 1:646703/140‑1 (MQ=255) tGACCGTCACCCATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc < 2:156074/143‑1 (MQ=255) cGTCACCGATCCCGAGACGAGGAGGATGGAGATCGACGGTCACGAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCaaaa < 1:778509/143‑1 (MQ=18) gTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACACCACGGCAAACAACAACGGCAAAac > 1:295272/1‑143 (MQ=255) cGATCCGGAGTCCATGGGTATCCAGATCGCCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCg < 1:135609/143‑1 (MQ=255) cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCGCCAACGGTGGCACCGGCACCCAGCAGAATGGTGCTCACGCCAGGACAAACAACAACGGCCAAGCGACCg > 2:207633/1‑143 (MQ=18) cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACgg > 1:668490/1‑132 (MQ=255) cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACgg < 2:668490/132‑1 (MQ=255) cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCGAAACCACCg > 1:154469/1‑143 (MQ=255) tggAGTCGATGGGTAAGCAGATCGACGGTGACAGGGCCATCGTCAATAACGAAGGCGAGAGCACCCTCACCAACGGGCGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc < 2:562724/142‑1 (MQ=18) cgcaGTCCATGGGTCTCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc < 1:458095/140‑1 (MQ=255) tCCATGTGTATCCAGATCGCCGGTCACAAGGCCATCGTCAATAACGAAGGCGACAGCACCATCACCAACGGTGGCACCGGCACCCAGTTTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa < 2:269652/143‑1 (MQ=18) tCCATGGGTAGCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGACCGACCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa < 1:601569/143‑1 (MQ=18) tATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGCCGGCAAGGATTCCa > 1:426890/1‑143 (MQ=255) tATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa < 1:351954/143‑1 (MQ=255) gccACATCCACGCCGACACGGCCCTCGTCAACAACGAAGGCGATAGCACCATCACCAACGGTGCCACCGTCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc < 1:152288/142‑1 (MQ=11) aTCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTAc > 1:805350/1‑143 (MQ=255) aTCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTAc > 1:199879/1‑143 (MQ=255) tCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGACTTTACGACGCTCGAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg > 1:187706/1‑143 (MQ=18) aCGGTGACAAGGCCATGGGCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCGAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa < 1:326171/143‑1 (MQ=255) aaGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgat < 2:288130/72‑1 (MQ=11) aaGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgat > 1:288130/1‑72 (MQ=17) ggCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACgg < 2:759203/80‑1 (MQ=255) ggCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACgg > 1:759203/1‑80 (MQ=255) ggCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGta > 1:484088/1‑143 (MQ=255) gCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCCCGGGCACGCGGATGAATGGTGGTGACGCCCCGGCAAACAACCACGGCAAAACAACCGCTAACGGCAAGGATTCCACCGGGACGGACACCAAccggaa > 2:260603/1‑138 (MQ=25) cATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg > 1:287366/1‑108 (MQ=255) cATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg > 1:92921/1‑112 (MQ=255) cATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg < 2:92921/112‑1 (MQ=255) aaTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGaaa > 1:6707/1‑143 (MQ=255) gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGATATCAACGGTGATAACGGAAAACTTATTCCGGa > 2:293851/1‑143 (MQ=255) agCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACg > 1:144773/1‑54 (MQ=255) agCACCATCACCAACGGTGGCACCGGCACCCAGATTAACGGTGATGACGCCACg < 2:144773/54‑1 (MQ=255) gCACCATCACCAACGGTGGCACCGGCACCCAGATTAAGGGGGATGACGCCACGGCAAACAGCAACGGCAAAACCACCGTTGACTGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGc < 1:545440/143‑1 (MQ=255) tCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATGGACGGTAATAACGGGAAGGTGATTCAGGACGGCGATCTg > 2:264412/1‑143 (MQ=11) ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt > 1:778893/1‑143 (MQ=255) cAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACCACGGCAAAACCACCGTTGACGGCAAGGATCCCACCGGTAGGGAAATCCACGGCAATAACGGGAAGGCGAGACAGGGCGGCGAGCTgggcg > 2:748721/1‑140 (MQ=11) cAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGGCGGCGATCTGGagg > 2:20908/1‑141 (MQ=255) accGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGt < 1:817361/141‑1 (MQ=255) ccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg > 2:361199/1‑57 (MQ=40) | TCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGT > NC_000913/1466194‑1466461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |