Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,712,338 A→T 9.6% intergenic (+180/‑431) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,3380AT 9.6% 104.3 / 3.2 41intergenic (+180/‑431)nth/dtpAendonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base T (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

TCTTTATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAA  >  NC_000913/1712197‑1712476
                                                                                                                                             |                                                                                                                                          
tctttATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAg                                                                                                                                           <  2:107462/143‑1 (MQ=255)
  tttATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGAt                                                                                                                                         >  2:251797/1‑143 (MQ=255)
  tttATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCAGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTATTAGCGTAATTTTATATTAATTTGTGATCAAgtt                                                                                                                                         >  2:115701/1‑141 (MQ=255)
      tcctccTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACg                                                                                                                                     <  2:307326/143‑1 (MQ=255)
      tCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCTTGATCACg                                                                                                                                     <  1:760808/143‑1 (MQ=255)
      tCCGCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTGAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAAGTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCTTGATCACg                                                                                                                                     <  1:372716/143‑1 (MQ=255)
      tCCACTTTTAAAACATCTCTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACg                                                                                                                                     <  1:238755/143‑1 (MQ=255)
        ctctTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGAt                                                                                                                                   >  2:641346/1‑143 (MQ=255)
              tAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTc                                                                                                                             <  1:602483/143‑1 (MQ=255)
               taaaCACCCTTTTAACGCCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCg                                                                                                                            <  1:359027/142‑1 (MQ=255)
                  aCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCaa                                                                                                                         >  1:589123/1‑143 (MQ=255)
                  aCATCTGTTTAATGTGAACGATGCCAGTGCTTAGCGGTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCaa                                                                                                                         <  2:516173/143‑1 (MQ=255)
                    aTCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATg                                                                                                                       >  2:122762/1‑143 (MQ=255)
                                       tgCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATc                                                                                                    <  1:251797/143‑1 (MQ=255)
                                                   gCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCa                                                                                        <  1:122762/143‑1 (MQ=255)
                                                      ttATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGCACTTTTCGATGCGTTCGTGCTCGAGATCACGATGCTGTCGCAACGTAAAAACATTGCAAGAATCAGCTTTAAATCagct                                                                                     >  2:804498/1‑140 (MQ=255)
                                                      ttATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCTTGATCAAGATCACGATGCTGTCGCAATGTAAAGACGTTGCAAGAATCAGGTTAAAATCAttt                                                                                     >  1:232893/1‑143 (MQ=255)
                                                        atcatcAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTaa                                                                                   <  2:611742/143‑1 (MQ=255)
                                                          catcaGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAAt                                                                                 >  1:356217/1‑143 (MQ=255)
                                                            tcaGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAGATCATTCAAAttt                                                                               >  1:554912/1‑143 (MQ=255)
                                                             caGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTa                                                                              <  1:526057/143‑1 (MQ=255)
                                                                    tCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGc                                                                       <  1:723265/143‑1 (MQ=255)
                                                                       gTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAGTTTACACACGcaac                                                                    >  1:293677/1‑143 (MQ=255)
                                                                        tttGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCATGATCACGATGCTGTCGCCATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGccacc                                                                   >  2:624491/1‑142 (MQ=255)
                                                                        tttGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGcaaca                                                                   >  2:418987/1‑143 (MQ=255)
                                                                           gCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAat                                                                >  1:691576/1‑143 (MQ=255)
                                                                                 caCCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGAc                                                          <  1:46499/142‑1 (MQ=255)
                                                                                       aaaTGCAGGTTAATTGTTTTTTTAATAGCGAATTTTTCTATTCATTCGTGATCAAGATCACGCTGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACaa                                                    <  1:414409/143‑1 (MQ=255)
                                                                                         aTGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACaaaa                                                  <  2:179275/143‑1 (MQ=255)
                                                                                                          ttttAATAGCGAAATCTTCTAGTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAAGCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTAttt                                 <  1:32098/143‑1 (MQ=255)
                                                                                                            ttAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTAttttt                               <  1:145131/143‑1 (MQ=255)
                                                                                                             tAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTc                              <  2:473639/143‑1 (MQ=255)
                                                                                                             tAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTAGCTAttttta                              >  2:273168/1‑142 (MQ=255)
                                                                                                                tAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGATATTTTTCAGa                           >  1:328355/1‑143 (MQ=255)
                                                                                                                               ttcattcGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCa            >  1:589372/1‑143 (MQ=255)
                                                                                                                               ttcattcGTGATCAAGAGTGCGGTGCTGTCGCAATGTAAAAACAGTGCAAGAATCAGGTTAAAATCATTTAAATTTACGCACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACAGGACTATCTCa            <  2:486742/143‑1 (MQ=255)
                                                                                                                               gtcattcGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAGCTCATTTAGAGTTACACACGCACCAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCa            <  2:98296/142‑1 (MQ=255)
                                                                                                                                  catcGTGATCTTGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTa         >  1:251694/3‑143 (MQ=255)
                                                                                                                                      gTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTACAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTACTaa     >  2:23542/1‑143 (MQ=255)
                                                                                                                                      gTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGCCCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTaa     >  2:39943/1‑143 (MQ=255)
                                                                                                                                         aTCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTaaaaa  <  1:764257/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
TCTTTATCCTCTTTTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTGTCGCAATGTAAAAACATTGCAAGAATCAGGTTAAAATCATTTAAATTTACACACGCAACAAATATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAA  >  NC_000913/1712197‑1712476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑