Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,934,900 C→T 50.1% G472E (GGA→GAA)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,9000CT50.1% ‑0.9 / 25.1 29G472E (GGA→GAA) zwfNADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (3/11);  new base T (5/9);  total (9/20)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCA  >  NC_000913/1934761‑1935041
                                                                                                                                           |                                                                                                                                             
tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                                                                                                                            >  1:309945/1‑143 (MQ=255)
        tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGAAACGACCACCACGGGTAATCATCGCCACCAAAGCAACGGGTCCCCAgcgcccg                                                                                                                                    >  2:304293/1‑137 (MQ=255)
         aaaaaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg                                                                                                                                   <  1:700657/143‑1 (MQ=255)
          aaaaTTGTTCTACAATCTGCGCAAGATCATGTTAACGGTAAAATAACCATAACGGAGCAGAGCAGATATTACTCAACCTCATTCCAGGAACGACCATCACGGGTACTCAACGCCACCGCGGACACGGGCACCCAGGTCCCGGc                                                                                                                                  >  2:226853/1‑143 (MQ=255)
               cgTTCTCCATTGTGCCCAAGATCGTGTTACCGGTAAAATAACCATACAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCACCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGAt                                                                                                                             <  2:749134/142‑1 (MQ=255)
                  tCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAGACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACg                                                                                                                          <  2:778786/143‑1 (MQ=255)
                  tCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACg                                                                                                                          <  1:506457/143‑1 (MQ=255)
                             cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCAGCACGGGTAATCATCGCCACCGATGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGca                                                                                                               <  1:139715/143‑1 (MQ=255)
                              gcgaGATCATGTTATCGGTCCGATAACCGTAATGGGTAAGCGCAGATATTACTCAAACTCATTCCAGGAACGTCGTTCACGTGTTATGATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGcat                                                                                                              <  2:683831/140‑1 (MQ=255)
                              gcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGCGGCAACGGGACCCCCGCTTCCGGCCTCACACGGTTTCGGCGcac                                                                                                              >  2:695794/1‑142 (MQ=255)
                                           aCCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGc                                                                                                 <  1:8325/143‑1 (MQ=255)
                                           aCCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGc                                                                                                 <  2:216117/143‑1 (MQ=255)
                                                              aGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAg                                                                              <  1:757992/143‑1 (MQ=255)
                                                                     cgcctgttatTACTCAAACTCAGTCCAGGAACTACCATCACGGTTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTAccc                                                                       <  2:277773/136‑1 (MQ=255)
                                                                       gcAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTACGAAGCATCATTGTCCATCGCCGACACATCAGTAACGGAGTCAACACAt                                                                     >  1:69728/1‑143 (MQ=255)
                                                                        cAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCCGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCAtt                                                                    >  1:676683/1‑143 (MQ=255)
                                                                                 cTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGctt                                                           <  1:457936/143‑1 (MQ=255)
                                                                                                agaccGACCATAACGGATAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTcgc                                            <  1:634465/139‑1 (MQ=255)
                                                                                                  gacggtcATCACGGGCAATCATCGGCACCGAGGCATCGGGTTCCCAGGTTCCGGCCTGATACGGGTTCGGCGCATCATTGTCCATCGCGCACGCCTCAGTAATGGAGTCTCCCCATTTCCAGGCTTCTTCCACTTCGTCGCGa                                          <  2:100923/137‑1 (MQ=255)
                                                                                                     gACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCGTCGCCCACGCGTCAGTAATGGAGGCTAGCCATTTCGAGGCTTCTTCCACTTCGTCGGGACGt                                       >  1:581064/1‑143 (MQ=255)
                                                                                                     gACCACCAGGGTTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATCGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGt                                       <  2:662135/143‑1 (MQ=255)
                                                                                                               ggTAATCGTCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTg                             <  2:225690/143‑1 (MQ=255)
                                                                                                                    tcagcGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGa                        <  2:24933/143‑1 (MQ=255)
                                                                                                                       tcGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGCTAACCACCCATTTCCAGGCTTCTTCCACTTCGTCCCGACGTCCGACCCGTGCCTGAATa                     >  1:600938/1‑143 (MQ=255)
                                                                                                                         gCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCGATTTCCAGGCTTCTTACACTTCGTGGCGACGTGGAAACGGGGCGTGattggt                   >  1:745170/1‑138 (MQ=255)
                                                                                                                              cGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCCCAGTAATGGAGTCTACCCATTTCCAGGCATCTTCCACTTCGTCGCGACGTACCAACCGTGCCTCACTAcccccca              >  2:639270/1‑138 (MQ=255)
                                                                                                                               gAGGCGACGGGGTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAGGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCAt             <  2:667005/143‑1 (MQ=255)
                                                                                                                                     aCGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTc       <  2:417830/143‑1 (MQ=255)
                                                                                                                                      cGGGTTCCCAGGTTCCGGCCGGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTcc      <  1:570387/143‑1 (MQ=255)
                                                                                                                                         gTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCcagc   <  1:90319/143‑1 (MQ=255)
                                                                                                                                          gccccAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCGTCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCcagca  <  2:309945/142‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
TGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCA  >  NC_000913/1934761‑1935041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑