| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,447,483 | G→T | 67.4% | intergenic (‑10/+25) | aroC ← / ← prmB | chorismate synthase/50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,447,483 | 0 | G | T | 67.4% | 31.6 / 21.7 | 35 | intergenic (‑10/+25) | aroC/prmB | chorismate synthase/50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (6/6); new base T (16/7); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01 | |||||||||||
TTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCC > NC_000913/2447348‑2447619 | ttGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTGAttgt > 1:714960/1‑143 (MQ=255) gTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTGGTCCAATTGTGGTTCCAGCCATCACGGCTCCTTTATTGTTGTGtt > 2:39042/1‑143 (MQ=255) ccGGCAGCGAGATGCCTGGCGGAACACCATCGACGAGGCAGCCGGGCGCCAGCCCGTGCGATTCGAGCCAGGTGGTGACGCTATAGCGTTGTCCAATTGGGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGt‑t < 2:290447/143‑1 (MQ=255) cccgCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTAc < 1:105759/140‑1 (MQ=255) aggCCGGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGTAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCtt < 2:530794/141‑1 (MQ=255) tGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCttt < 1:354705/143‑1 (MQ=255) tGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATaa > 1:305754/1‑143 (MQ=255) ggCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGGGT‑TTACTTCATCTTTATaaa > 2:451275/1‑143 (MQ=255) acgaTGCAGCCGACCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTtctc < 2:821457/143‑1 (MQ=255) aGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTCATCAATCGCGAAATGTTCTCGTGCGGc > 2:792621/1‑143 (MQ=255) ggggcgcCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCGATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAAt < 2:699062/140‑1 (MQ=255) ccaGCGCCCGCCCGTCCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCGCCTTTATTGTTGTGTTTGCGTGT‑TGACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAAt < 1:402695/141‑1 (MQ=255) cAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAgctgc > 1:632385/1‑143 (MQ=255) aGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAATGAGTTGTCCATTTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAgctgct < 2:646647/143‑1 (MQ=255) gCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTGCTTAATCTTTATAACTCGCGACATGGTCTCGTGCGGCAATCAgctgccc > 2:323385/1‑141 (MQ=255) gCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTTTGTTATTCTCGAGAACGCGTGTATCACAT‑TGCATTAGTAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGt < 1:604267/143‑1 (MQ=255) ttCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGc > 2:160984/1‑143 (MQ=255) ccGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGCAATGTTCTCGTGCGGCAATAAGCTGCTCTCTGGTGAGcggca > 2:810368/1‑139 (MQ=255) gAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGCTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGG‑TTACTTAATCTTTATACATCGCGAAATGTTCTGGTGCGGCAATAAGGTGATGTATGGTGGACATaaaa > 2:237941/1‑142 (MQ=255) taggcgAAGGAGTTGTCCGATTGTGTTTCCAGCCATCGCGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATcg < 2:638648/140‑1 (MQ=255) tACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATcg < 2:632385/143‑1 (MQ=255) gcgAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATcgccg > 1:816770/1‑143 (MQ=255) tGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCaa > 1:609213/1‑143 (MQ=255) gTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCaaa < 1:160984/143‑1 (MQ=255) tCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATAGCCGCCGTTATCAAAc > 2:393165/1‑143 (MQ=255) cAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TGACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGTGCATAAACACACCATCGCCGCCGTTATCGAACTc > 1:806310/1‑143 (MQ=255) gtgtTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAACTGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCACCGCCGCCGTCACCAAACTccggcc > 2:80326/1‑143 (MQ=255) gtgtTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACCCACCATCGCCGCCGCTATCAAACTccagcc > 2:779127/1‑143 (MQ=255) gtTTCCAGCCATCACGGCGCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTccagccag < 1:171292/143‑1 (MQ=255) ttCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGt > 1:693710/1‑143 (MQ=255) ttCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGt > 1:454988/1‑143 (MQ=255) tCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGAAATAAGCTGCTCTTAGCTGAACATACACCCACCATCGCCGCAGTTATCAAACTccagcgcggcg > 2:665458/1‑137 (MQ=255) ccAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGa > 1:280438/1‑143 (MQ=255) cACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACATACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACa > 1:212612/1‑143 (MQ=255) ggCTCCTTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCCAACTCCAGCCAGGTGAACGGAACATcc > 1:52784/1‑143 (MQ=255) | TTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGT‑TTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCC > NC_000913/2447348‑2447619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |