| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,518,275 | T→G | 8.9% | intergenic (‑44/‑192) | alaW ← / → yfeC | tRNA‑Ala/putative DNA‑binding transcriptional regulator YfeC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,518,275 | 0 | T | G | 8.9% | 126.4 / 2.2 | 48 | intergenic (‑44/‑192) | alaW/yfeC | tRNA‑Ala/putative DNA‑binding transcriptional regulator YfeC |
| Reads supporting (aligned to +/- strand): ref base T (27/16); new base G (2/3); total (29/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTGTTATAA > NC_000913/2518136‑2518410 | aGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCGGACCTCTGGCATGCCATGCAAGCGCCCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCg < 1:256450/143‑1 (MQ=255) cTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGaa < 1:797306/143‑1 (MQ=255) tGGGTGAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAAt < 2:520655/143‑1 (MQ=255) tGGGGGCAACATTTGGTGGAGCTAAGCGGGATCGAACCGCTGCCCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAAt < 2:112950/143‑1 (MQ=255) gggTGCCACATTTGGGGGAGCTAAGCGGGATCGAACCGCTGACCTCGTGCATGCCATGCAAGCGCTCCCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGGCGAGTTGACGGGCGGAATTCATATG‑ATGCCGCCCgtcaa < 2:395549/143‑5 (MQ=18) ggTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAAtgt < 1:450040/143‑1 (MQ=255) cAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCC‑TCATATGAATTCCGCCCGAATGTGTCa > 1:651462/1‑143 (MQ=255) cAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCa > 2:382380/1‑143 (MQ=255) acaggttGGTCGGGGTAAGGGGCCTCGAACCGCTGACGACTTGCATGCCTTCCAAGCGCTCTTCCAGCTGCGCGTTAGCCCCGTCACGTAAAGCTTGACGCGTCGTAAGTCACGA‑TTTTATGAATTCCGCCCGAATGTGTCaa < 2:209497/138‑1 (MQ=255) aTTTGGTGGAGCGAGTCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACgg < 2:511716/143‑1 (MQ=255) tggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGCATGTGTCAACGGCAAAta > 2:519960/1‑142 (MQ=255) gAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCCCCGGCCCATTGc > 1:628167/1‑143 (MQ=255) gCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCACGAATGTGTCAACGGCAAATTGaaa > 1:230038/1‑143 (MQ=255) cTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAAc > 1:160068/1‑143 (MQ=255) tAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAACCCTTGTCGAGTTGACGGGCGGCA‑TCAAATGAATTCCGCCCGCATGTGGCACCGGCGAACTGccagg > 2:196375/1‑139 (MQ=21) gAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCaa < 1:536351/143‑1 (MQ=255) aaCCGCTGACCTCTTGCATGCCATGCAAGCGCTCGCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAAt < 2:108983/143‑1 (MQ=255) tctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGCTTCAATCGCTGAAAAAt > 2:187153/1‑143 (MQ=255) tctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGGAAAGCTTGTCGAGTTGAGGGGCGGCA‑TCATCTGAATTCCGCCCGAATGTGTCAACGGCAAATTTCAACACGGAGCTTCAACCGCCGAAAAAt > 2:136283/1‑143 (MQ=37) ttGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCACCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGGATGTGTCCACGGCCATTTGCATAGTGTCGTTTCCCTCGCTTAACACTCa > 1:15615/1‑143 (MQ=25) ccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGAATTCATATG‑ATGCCGCCCg < 1:632074/86‑1 (MQ=25) ccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGAATTCATATG‑ATGCCGCCCg > 2:632074/1‑86 (MQ=25) cgcTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAAGGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAGATGAAGAAttt > 1:382900/1‑143 (MQ=255) gcgctcCCAGCTGAGCTATAGCCCCGTCACGTAAAGCGTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAAtttt < 1:269440/140‑1 (MQ=255) gcTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAAtttt < 2:773628/143‑1 (MQ=255) ctctCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTg > 1:769139/1‑143 (MQ=255) tctcCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAAGCAGGCAAATGAACAATTTTgg > 2:708812/1‑143 (MQ=255) cccAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAAGGAACAATTTTGGaaa > 1:295989/1‑143 (MQ=255) aGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAAcc > 1:757847/1‑143 (MQ=255) ataGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCAGCCCGAATGTGTCAACGGCACATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCAtt > 1:224519/1‑143 (MQ=255) ggCCCCGTCACGTAAAGCTGGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCa < 2:190565/142‑1 (MQ=255) aGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCa < 1:493562/143‑1 (MQ=255) ccccGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt > 1:82693/1‑143 (MQ=255) ccccGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAACCAGGCAAATGAAAAATTTTGGAACACCACTCGCATGCagt > 2:476458/1‑143 (MQ=255) aaGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGt > 2:181119/1‑143 (MQ=255) gCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCCTGTCa > 2:76176/1‑143 (MQ=255) gtGTCGAGTTGGCGGGCGGCA‑TCATATGAATGCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACg < 2:628167/142‑1 (MQ=255) gTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCCTGTCACggc > 1:343200/1‑142 (MQ=255) aGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACACTTTTGGAAAACCGCTCGCATACAGTACTTATTCCTGTCACGGTTccc > 2:85286/1‑143 (MQ=255) aGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTGAAATCGCTGAAAAATCAGGCAAAGGAAGAATTTTGGAAGACCGCTCGCATTAAGAGGGGATGCATGTGACGGtatac > 1:319581/1‑139 (MQ=255) gTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCt < 1:533740/143‑1 (MQ=255) gTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCt < 1:359633/143‑1 (MQ=255) gTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCt > 1:702539/1‑143 (MQ=255) gTTGACGCGCGGCA‑TCATATGAATTCCGCCCGAATCTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCt < 2:424749/143‑1 (MQ=255) gggCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGACAACTCAGGCAAATGAACAATTTTGGAAAACCGCACCCATTCAGCACGTATTCACGTCACGGTATCCTGTaacg > 2:458979/1‑141 (MQ=255) gcggaattcatATG‑ATGCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGATTCCTGTAAAgtg > 1:43071/8‑143 (MQ=255) atatGAATTCCGCACGAATGTGTAAACAGCAAAATGCAACGTGTAGTTTAAATCGCTGAAAAATAAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTAATGCACCGAATTCCTGAAAAGTAGAGAtata > 2:217828/1‑143 (MQ=255) tatGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTGTTATaa > 2:232627/1‑143 (MQ=255) | AGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCA‑TCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTGTTATAA > NC_000913/2518136‑2518410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |