Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,785,436 A→G 9.1% intergenic (‑425/+326) ypjC ← / ← ileY DUF5507 domain‑containing protein YpjC/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,785,4360AG 9.1% 124.4 / 3.1 49intergenic (‑425/+326)ypjC/ileYDUF5507 domain‑containing protein YpjC/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base G (3/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

AACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCC  >  NC_000913/2785295‑2785577
                                                                                                                                             |                                                                                                                                             
aaCGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCGTCCTTACTCTACGGCATTGTTCTACATACACTAGTTGTGGTACTCACTTATCGTCCGTGAGCGag                                                                                                                                              >  2:408189/1‑142 (MQ=255)
     tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACagag                                                                                                                                         <  1:443372/143‑1 (MQ=255)
     tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGAAGATGTTTAAGGAGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCGCTTATCATCAGTGAGCGGACagag                                                                                                                                         >  1:161914/1‑143 (MQ=255)
       acaAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTCCATACATTGGTTGTGGTCCTCACTTATCCTCAGTGAGCGAACCgagag                                                                                                                                       >  2:598826/1‑142 (MQ=255)
         aaaaCTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATa                                                                                                                                     <  1:763010/143‑1 (MQ=255)
         aaaaCTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATa                                                                                                                                     <  1:536120/143‑1 (MQ=255)
               cATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAg                                                                                                                               <  1:706661/143‑1 (MQ=255)
                         cggcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGt                                                                                                                     <  1:285789/143‑1 (MQ=255)
                           gcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTaa                                                                                                                   >  2:272171/1‑143 (MQ=255)
                                  ttCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCt                                                                                                            >  1:387897/1‑143 (MQ=255)
                                   tCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAGTTAACCTg                                                                                                           >  1:419630/1‑143 (MQ=255)
                                     tGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTAGAGTAATTAACCTGAt                                                                                                         >  1:730728/1‑143 (MQ=255)
                                        tCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACCTTGGTTTTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTaa                                                                                                      >  1:737888/1‑143 (MQ=255)
                                            tagtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGa                                                                                                  >  2:431721/1‑143 (MQ=255)
                                              gtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAg                                                                                                <  1:240957/143‑1 (MQ=255)
                                               tagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGGACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAgg                                                                                               >  1:677268/1‑143 (MQ=255)
                                               tagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGGACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAgg                                                                                               >  1:677272/1‑143 (MQ=255)
                                                agATGTCTACGGCGTCGCAGACACATTTCACACTTACTCTACTGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGCGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAggg                                                                                              <  1:43759/143‑1 (MQ=255)
                                                acaTGTTTGAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAggg                                                                                              <  1:826815/141‑1 (MQ=255)
                                                          ggCGTGGCAGAGACCTTTCCTCCTTACTCTACGGCAATCGGCGACATCCATAGGCGGTGGTACTCACTTAACACCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACATGATTAAATGAAGGGGTATAATaaa                                                                                    <  1:7013/143‑1 (MQ=255)
                                                          ggCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATaaa                                                                                    <  2:222994/143‑1 (MQ=255)
                                                                gCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGataa                                                                              >  2:678946/1‑143 (MQ=255)
                                                                         tttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGc                                                                     >  1:162140/1‑143 (MQ=255)
                                                                           tCATCCTTACTCGACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCtt                                                                   <  1:241104/143‑1 (MQ=255)
                                                                               ccTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATc                                                               <  1:761969/143‑1 (MQ=255)
                                                                                  tACTCTACGGCATTGTTCTACATACATTGGTCGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTACTTACCCTGATTAAATGCAGGGGTATACTACATGATAAACCTCTGGCTTTATCGtt                                                            >  2:100870/1‑143 (MQ=255)
                                                                                    cctgaCGGCATTGTTTTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTaa                                                          <  2:111691/139‑1 (MQ=255)
                                                                                     tctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAt                                                         >  2:534664/1‑143 (MQ=255)
                                                                                      ctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGCATAGTTCAGTGCTTTGAGTAATTACCCTGAATAAATCAAGGAGTAAAATAAATGATAATACTCTGGCTTTGTAGTTAAtt                                                        >  2:757991/1‑143 (MQ=255)
                                                                                         cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGGACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACt                                                     <  2:677268/143‑1 (MQ=255)
                                                                                         cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGGACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACt                                                     <  2:677272/143‑1 (MQ=255)
                                                                                         cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACt                                                     <  1:759925/143‑1 (MQ=255)
                                                                                         cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTACCGTTAATTACt                                                     >  2:201313/1‑143 (MQ=255)
                                                                                             aTTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATAATATGGATTTATCGTTAATTACTTAAt                                                 >  2:64465/1‑143 (MQ=255)
                                                                                                gTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATTCTCTGGCTTTATCGTTAATTACttccttcc                                              >  2:239135/1‑143 (MQ=255)
                                                                                                     acatacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATg                                         >  2:103204/1‑143 (MQ=255)
                                                                                                           atTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTCACCTGAGTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCa                                   >  1:307715/1‑143 (MQ=255)
                                                                                                               gTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGACGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTACGTAAttt                               >  2:782013/1‑143 (MQ=255)
                                                                                                                ttGTGGTACTCCCGTATCACCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTg                              <  1:744940/143‑1 (MQ=255)
                                                                                                                  gtgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGcc                            >  1:539917/1‑143 (MQ=255)
                                                                                                                   tgttACTCACTTATCATCAGTGAGCGAACAGATAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGccc                           <  2:49061/140‑1 (MQ=255)
                                                                                                                      tACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCt                        >  2:67203/1‑143 (MQ=255)
                                                                                                                      cgcTCACTGATGATAAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCGGCt                        >  1:727450/3‑143 (MQ=255)
                                                                                                                       aCTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTGTCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGAtt                       <  1:272171/143‑1 (MQ=255)
                                                                                                                           aCTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCa                   >  1:693457/1‑143 (MQ=255)
                                                                                                                            cTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCAt                  <  2:480471/143‑1 (MQ=255)
                                                                                                                             ttATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTCTAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTGATTCCACATGTAAGCAATTTGCCCGCTTGGCATa                 <  1:431721/143‑1 (MQ=255)
                                                                                                                                          gCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCActtttt    >  1:476231/1‑143 (MQ=255)
                                                                                                                                           cGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTc   <  1:196115/143‑1 (MQ=255)
                                                                                                                                            gAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTcc  <  1:149020/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
AACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCC  >  NC_000913/2785295‑2785577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑