BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	111.8 / NA	39	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (22/17); total (22/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATT  >  NC_000913/3041258‑3041529

aTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTg                                                                                                                                   >  1:459229/1‑143 (MQ=255)

    ctctGTTTCCATTAGTCATTAGTACGCCGTTAAGCACCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCTTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                               <  2:643148/143‑1 (MQ=255)

        tttACGATGAGTCATTAATACGCCGTGAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGGCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAtt                                                                                                                           <  1:800822/143‑1 (MQ=255)

         ttACCATTAGTTATTAATACGCCGTTACCCAGCTCAGGACTCTATTATGGCTTTTACACCTTCTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTa                                                                                                                          <  1:415633/143‑1 (MQ=255)

            ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCACCCCGTCCGCCTCCGGCCTCTGCCCTTTTACCGCTCACGCTGATGACGCTTGATGACTGGGGGCGTGCCACCATTACtc                                                                                                                       >  2:77919/1‑142 (MQ=255)

              aTTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCGCCATTACTggg                                                                                                                     >  1:81407/1‑142 (MQ=255)

              aTTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGAGCGTGCCACCATTACTGgc                                                                                                                     >  1:317464/1‑143 (MQ=255)

                   tCATTTATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGTGGACTCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                <  1:174381/143‑1 (MQ=255)

                   tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                >  1:529985/1‑143 (MQ=255)

                     aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTATGCCCGTTTACCGCTGACGCTGATGACGCTTGAGGACTGGGCGCGGGCCACCATTACTGGCGCGGACa                                                                                                              >  1:783116/1‑143 (MQ=255)

                     aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTAAACCTTTGCGGCCCCGTCAGACTAAGACCACACCTCTGTTACCGCTGACGCTGATGACGCTTGGTGACTGGGCGCGTGCCACCATTACTGGCGCGGCCa                                                                                                              >  1:472219/1‑143 (MQ=255)

                      ttAATACGCCGTTAAGCTACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAg                                                                                                             <  1:140285/143‑1 (MQ=255)

                      ttAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCggcccg                                                                                                             >  1:658885/1‑139 (MQ=255)

                       tAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTCCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGc                                                                                                            <  1:624032/143‑1 (MQ=255)

                        aaTACGCCGTTAAGCAACTCAGGACTCTATTATGGTTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCGGACGCTGATTACCCTTGATGACTGGGGGCGGGCCACCACTACTGGCGCGGAGAGCg                                                                                                           >  2:176549/1‑143 (MQ=255)

                          tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCGACGGCTTCTGCCCGTTTACCGCTGACCCTGATTACCCTTGATGACTGGGCGCGTGCCACCATTACTCGCGCGGCCAGCGac                                                                                                         >  2:717263/1‑142 (MQ=255)

                            cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGCCGCTGCTGCCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCTGACAGCGacac                                                                                                       >  2:186155/1‑140 (MQ=255)

                                   aaGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTAACGCCGACGCTGATGACGCTTGATGACTGGGGGGGGCCCACCAACACTGGCGCCGACAGCGACAAatagaat                                                                                                >  2:169171/1‑139 (MQ=255)

                                     gCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGCGCCACCATTACTGGCGCCGACAGCGAAAAACATATGCa                                                                                              >  2:379999/1‑143 (MQ=255)

                                        aCTCAGGACTCTATTATGGCTTTTACACCTTTCCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCGGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAggg                                                                                           <  1:253218/143‑1 (MQ=255)

                                           cAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCa                                                                                        <  1:355430/143‑1 (MQ=255)

                                             ggACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg                                                                                      <  1:775263/143‑1 (MQ=255)

                                             agACTCTATTATGGCTTTTACCCCTATTCCACCCCGGCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTGGAGGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg                                                                                      <  1:366164/142‑1 (MQ=255)

                                                 tctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATCACTGGCGCGGACAGCGAAACATATATGCAGGGTCAGGTGAc                                                                                  >  1:584491/1‑143 (MQ=255)

                                                     ttatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                                                              <  1:237343/143‑1 (MQ=255)

                                                        tGGCTTTTACACCTTTTCCGCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                                                           <  1:619414/143‑1 (MQ=255)

                                                                  acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCCTGATGACTGGGCGCGTGCCACCCTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGa                                                                 >  1:722700/1‑143 (MQ=255)

                                                                   ccTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCCAGGTCAGCTGAACGCAGCTCTCAGCCAGAt                                                                >  2:181490/1‑143 (MQ=255)

                                                                     tctccccccccGTCAGCCTACGGCTTCTGCCCGTTTACGGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                              <  1:105620/136‑1 (MQ=255)

                                                                             cacGTCAGAATGCGGCTTCTGCCCGTTTACCGCTGGCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                      <  2:317464/141‑1 (MQ=255)

                                                                                   aGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAc                                                <  1:36708/143‑1 (MQ=255)

                                                                                          ggCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcg                                         >  1:687199/1‑143 (MQ=255)

                                                                                           gcgtCTGCCCGTTGACCGCTGGCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCCAAAACCACGAAAAATATATGCAGGGTCATGTGACAGCAGATGGCAGCCAGATGGCAGAAGATCAGCACCTGCTcgc                                        <  1:738297/140‑1 (MQ=255)

                                                                                             ttCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGCACAGGGAAAAATAAATGCAGGGTCAGGTGACACCAGATGTCAGCCAGATGGCAGAAGAGCAGCACCTACTcgccg                                      >  2:242644/1‑143 (MQ=255)

                                                                                                 gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACCGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCgccgccga                                  >  2:771923/1‑141 (MQ=255)

                                                                                                        tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc                           >  2:442671/1‑143 (MQ=255)

                                                                                                             ctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGACAGGTGACTTCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaa                      >  1:820279/1‑143 (MQ=255)

                                                                                                                            cTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCCCCCATCGCGACGCCAAAGGTAAAATGTGGac       >  2:391565/1‑142 (MQ=255)

                                                                                                                                 tgaCTGGGCGCGTGCCAGCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt  <  2:788404/143‑1 (MQ=255)

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATT  >  NC_000913/3041258‑3041529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`