| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 639,694 | G→T | 21.1% | intergenic (+186/‑59) | ahpC → / → ahpF | alkyl hydroperoxide reductase, AhpC component/alkyl hydroperoxide reductase, AhpF component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 639,694 | 0 | G | T | 21.1% | 76.7 / 13.7 | 47 | intergenic (+186/‑59) | ahpC/ahpF | alkyl hydroperoxide reductase, AhpC component/alkyl hydroperoxide reductase, AhpF component |
| Reads supporting (aligned to +/- strand): ref base G (21/15); new base T (6/4); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CACCCCGGTCACTTACTTGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAG > NC_000913/639554‑639818 | cACCCCGGTCACTTACTTGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCa < 1:250379/143‑1 (MQ=255) ccGGTCACTTACTTGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGACATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCa > 1:189082/1‑143 (MQ=255) acttacttGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCAAATTTTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGc > 1:643089/1‑143 (MQ=255) ttacttGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCg > 1:247282/1‑143 (MQ=255) ttacttGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTCTCTTCCCCGCCCCATTATGCCGGCTTCGTCCTGGTAGCCg > 1:257/1‑143 (MQ=255) cttGTGTAACCTCTCGTGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCGCag < 2:659940/143‑1 (MQ=255) ttGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCaga < 1:805362/143‑1 (MQ=255) ttGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCaga < 1:569069/143‑1 (MQ=255) gtgtCAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAgg < 2:274658/143‑1 (MQ=255) gtgtAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAgg > 1:92480/1‑143 (MQ=255) cTCCCGGGGATTCACAGGCTAGCCGCCTTGCCCTGATGTGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCCGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTg < 1:640213/143‑1 (MQ=255) aTTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTGATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCGCAGAGGCCGCTTGCatgatgat < 1:350084/143‑1 (MQ=255) acaGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCAGGATGATGttt > 2:633063/1‑143 (MQ=255) ggCTAGCCGCCTTGCTCTGACGCGACATACTCCGGTAATTTACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTaaa < 1:221022/143‑1 (MQ=255) gCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTTTTCGTGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCCTGATGATGTTTAAAg > 1:156103/1‑143 (MQ=255) gCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTCCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAg > 2:628450/1‑143 (MQ=255) aGCCGGCGTGGTCTGACGCGAAAGACCTCGGAAATTCACCTAAGTCTCCGGGTGCTGCGGCACCCGATTTTCTACCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGccc < 1:239547/143‑1 (MQ=255) gccTGGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACACGATTTTCTTCCCCGCACCACGATGCCAGCTTCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGga < 1:587222/143‑1 (MQ=255) gccGTGCTCTGACGCGACATACTGCGGAAATTCACCTTATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGga < 2:269927/143‑1 (MQ=255) ctctGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTACAGCCCAGGAGATaaa > 2:205490/1‑143 (MQ=255) tGACGCGAAATACTTCGGAAATTCACCTAATTTTGCGGGTTCGTGGGCGCCGGAGTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACAt > 1:194545/1‑143 (MQ=255) tGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACac > 2:71018/1‑142 (MQ=255) cGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAatat > 2:686777/1‑143 (MQ=255) ggAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCACGCACCATGATGCAAGCTTGATCCAGGTAGCCGCAGAGGCCGCCTGCATGATGATGTTTAAAGCACAGCAGATAAACATGCCCCCCAAAAATATg > 2:760254/1‑143 (MQ=255) gAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGa < 1:292074/143‑1 (MQ=255) aTTCACCTAATTCGTCGGGGGCTGAGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGaaaa < 2:49047/143‑1 (MQ=255) cACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAaactc > 1:22008/1‑143 (MQ=255) ccTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGCTAAACATGCTCGACCCAAATATGAAaactcaa > 1:143004/1‑143 (MQ=255) cTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCACAGGAGATAAACATGCTCGACACAAGTATGAAaacacaac > 1:488354/1‑143 (MQ=255) tAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAaactcaac‑t > 1:403502/1‑143 (MQ=255) aaTTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCGCAGCGGCCGCTTGCATGATGATGTTCACAGCCCAGGAGACAAACATGCCCGACACAACTATGACaactaagc‑tc > 2:736473/1‑143 (MQ=255) aaTTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAACTAAGCAaacacaacgc > 2:210919/1‑141 (MQ=255) ttcgtcGGGTGCTGCGGCACCCGATCTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAActcaac‑tcaa < 2:227787/143‑1 (MQ=255) aatcGGGTGCCGCAGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAgg > 1:435356/3‑143 (MQ=255) ggTGCCGCAGCACCCGATTTTCTTCCCCGCACTATGATGCGAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGAGGATGTTTAAAGCCGAGGAGATAGACATGCTCGACACAAATATGAAAACTAAGC‑TCAAGGCTGAc > 2:428513/1‑143 (MQ=255) ggTGCCGCAGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCGTGCATGATGATGATTAAAGCCCAGGAGATAAACATGCTCGACACACATATGAAAACTCAAC‑TCAAGGCTTAc > 2:403880/1‑143 (MQ=255) gcGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTCGCCGCAAAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTgag > 1:692494/1‑143 (MQ=255) cGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATAGAAACTCAAC‑TCAAGGCTTACCTGgaga > 1:110346/1‑143 (MQ=255) aCCCGATGTTCTTCCCCGCACCATGAGGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTGAGAAAtt < 2:71409/143‑1 (MQ=255) ccGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCATC‑TCAAGGCTTACCTTGAGAAATTGa < 1:398987/143‑1 (MQ=255) ccGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTGAGAAATTGa < 2:643089/143‑1 (MQ=255) cGATTTTCTTCCCCGCACCATGATGCAAGCTTCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTGAGAAATTGAc > 1:821600/1‑143 (MQ=255) cGATTTTCTTCCCCGCACCATGATACAGGCTGCACCGAGGTAGCCGCAGAGGCCGCTCGCCTGATGATGTATAAAGACCACGAGATAAACATGCCCCAGAGACATATGACAA‑ACAACATCAAGTCTTACCTTGACAAACTGAc > 2:737291/1‑143 (MQ=255) ccccGCCCCCGGATGCAAGCTGCATCCAGGTGGCCGCAGAGGGCGCTTGCATGATGATGTTTAACGCCCGGGAGCGAAATCCGATCGACACAGATTTTAAGGCTCAAC‑TCAAGGCTTACCTTGAGAAATTGACCAAGCCTGtt < 2:804229/143‑1 (MQ=255) ccGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGa < 2:660929/143‑1 (MQ=255) ccGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTAGACACGAATTATTAATCGCAAC‑TCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGa > 1:599112/1‑143 (MQ=255) cGCACCATGAGGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGCTTAAAGCCCAGGAGATAAACATGCGCGACACAAATATGAAAACTCAAC‑TCACGGCCTACCTCGCGAAATGGACCAAGCCGGCTGAg > 2:768998/1‑143 (MQ=255) | CACCCCGGTCACTTACTTGTGTAAGCTCCCGGGGATTCACAGGCTAGCCGCCTTGCTCTGACGCGAAATACTTCGGAAATTCACCTAATTCTTCGGGTGCTGCGGCACCCGATTTTCTTCCCCGCACCATGATGCAAGCTGCATCCAGGTAGCCGCAGAGGCCGCTTGCATGATGATGTTTAAAGCCCAGGAGATAAACATGCTCGACACAAATATGAAAACTCAAC‑TCAAGGCTTACCTTGAGAAATTGACCAAGCCTGTTGAG > NC_000913/639554‑639818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |