| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,578,609 | T→G | 11.2% | I40L (ATT→CTT) | gntR ← | DNA‑binding transcriptional repressor GntR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,578,609 | 0 | T | G | 11.2% | 98.6 / 5.7 | 44 | I40L (ATT→CTT) | gntR | DNA‑binding transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base T (21/18); new base G (2/3); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01 | |||||||||||
ATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATA > NC_000913/3578473‑3578750 | aTTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTAGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGcc > 2:254241/1‑143 (MQ=255) gcgcgTAATACCTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGCTATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAAGATTGCcgcg < 1:29791/142‑1 (MQ=255) cgcgTCCTACTTCCGCGAACCCCTGGCTGGTCAGAGAAGGGAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGt < 1:69433/143‑1 (MQ=255) cgcgTAATCCTTCCGCGAAAACCTGGTTGGCGAGAGAAGGTAACCGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGt < 1:51745/143‑1 (MQ=255) cgcgGAATACTTCCGCGAAAACCTGGGTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTGAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAAGATTGCcgcgg < 1:499075/143‑2 (MQ=255) gcgTAATACTTCCGCGAACACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTa < 1:736164/143‑1 (MQ=255) cgTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCAGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTag > 1:603005/1‑143 (MQ=255) tAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTagag < 1:208983/143‑1 (MQ=255) aTACTTCCGCGACCACCTGGTTTGCGCGGCAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATAGCGGGCGCACGATTGGGAATAGAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCg < 2:709379/143‑1 (MQ=255) ttCCGCGAAAACCTGGTTGGTGAGAGAAGGTAAGAGTTGGTCAATCGCCCGGCTGGTGGCGGTAGAGAGGATATCGGGCGCACGATGGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCCATCTTGCGGCGTAGAGCGACgg > 2:497850/1‑143 (MQ=255) ttCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACgg > 1:88525/1‑143 (MQ=255) cgAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAAcc > 1:435033/1‑143 (MQ=255) gAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAACCCt > 1:560067/1‑143 (MQ=255) acagcgggttggtGATGGAGCGTAACAGGACGCCAAGCGCTCGGCTGGTCGCGTTAGGGAGCACAGCGGGCGCCCGATTGGGAATCTAGCCCAGTTCATCCATTGCCGCGGCTATCTTGACGCGTAGAGCGACGGAAACCTGc < 2:330542/137‑1 (MQ=255) cTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGGTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCg > 1:355871/1‑143 (MQ=255) gtGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCg < 1:325511/143‑1 (MQ=255) gtGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGAATAGGTCATCAAGAGCGGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCg > 1:513535/1‑143 (MQ=255) gaAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGCTGCGTaaaaa > 2:656151/1‑143 (MQ=255) aCGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGt > 1:647910/1‑143 (MQ=255) ttCGCCCGGCTGGGGGCGTTAGAGAGGATAGCGGGCGCACGATTGGGAATATAGCCCAGTTCAGCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCGGGGTTGCGTAAAAAACGGCTGACCGTCAtttt < 2:428523/142‑1 (MQ=255) tCGCCCGGCTGGTGGCGTTAGAGAGGATAGCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTg < 1:141957/143‑1 (MQ=255) gagagGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGc > 2:731776/1‑143 (MQ=255) gagatCGGGCGCACGATTGGGGATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACAt < 2:314710/140‑1 (MQ=255) cGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGa < 2:521398/143‑1 (MQ=255) gggCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAACCCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGaa < 1:451242/143‑1 (MQ=255) ggCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAg > 1:825394/1‑143 (MQ=255) cACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACg > 1:393354/1‑143 (MQ=255) aCGATTGGGAATATCGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACgg < 1:806530/143‑1 (MQ=255) aCGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTGACGCATACACGGTCAGCCACATCCTGAAGTACgg > 1:257591/1‑143 (MQ=255) cGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACCTCCTGAAGTCCggg > 2:638452/1‑143 (MQ=255) gggAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCAACATCCTGGAGTACCGGtcttc > 2:424405/1‑142 (MQ=255) atatAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtcttttctt > 2:819950/1‑143 (MQ=255) tataGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtcttttcttt > 1:484222/1‑143 (MQ=255) tataGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtcttttcttt < 2:88525/143‑1 (MQ=255) gtggCCCAGTTCGGCAAGCGGGGCGGCAAGTTTGCCGCGTGGCGCGCCGTAATCCTGCTCCGGGTTGCGTAAAAAACGGCTGACTGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTcttttctttt < 2:324971/140‑1 (MQ=255) cccAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGCAGTACGGGTCTTTTCTTTTTCAt > 1:813215/1‑143 (MQ=255) cAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTCGTTCATCg > 2:391920/1‑143 (MQ=255) gTTCATCAAGCGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTc < 1:181808/143‑1 (MQ=255) ttCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTACAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATGCTGAAGTACGGGTGTTTTCTTTTTCACCGTcc > 2:436081/1‑143 (MQ=255) tcaAGAGCCGCGGGACTCCTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGaa < 2:532218/143‑1 (MQ=255) tcaAGAGCCGCGGAAATCGTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGaa < 2:325814/143‑1 (MQ=255) tacgcgGCAAGATTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACa > 2:207471/3‑143 (MQ=255) acgcgGCAAGATTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACaa > 1:489492/2‑143 (MQ=255) cgcgGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGCTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACaaa < 1:254916/143‑1 (MQ=255) gtCTTGCCGCGTAGTTGGACTGAAACCTTCTCCGGGTTGCGTAAGCAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATACTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTGCAAAAGagata < 2:332493/142‑1 (MQ=255) | ATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATA > NC_000913/3578473‑3578750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |