| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,602,498 | T→C | 16.0% | I84V (ATT→GTT) | ftsE ← | cell division protein FtsE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,602,498 | 0 | T | C | 16.0% | 44.4 / 4.0 | 25 | I84V (ATT→GTT) | ftsE | cell division protein FtsE |
| Reads supporting (aligned to +/- strand): ref base T (10/11); new base C (2/2); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01 | |||||||||||
GCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCG > NC_000913/3602356‑3602634 | gCTTTGTCCAGCAGCCCGACTGCATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAAt < 2:378112/143‑1 (MQ=255) ggTCCAGTAGCCCGACTGTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATg < 1:256788/142‑1 (MQ=255) gTAGCCCGACTTTATCCGGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATc < 1:559359/143‑1 (MQ=255) ccGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTggcg > 1:383451/1‑143 (MQ=255) tttATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCa < 1:683063/143‑1 (MQ=255) cAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTCGATGGTCATCCTGGAAAATCATGCCTATCTAGCGACAAAGaaaaa > 1:387451/1‑141 (MQ=255) cAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACg < 1:577443/143‑1 (MQ=255) ccgACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCTGTCAGTAGATGGTGATCCTGGAAAACCATGCCAATCTGGCGGCGCAGAAACGGAACTTCa > 2:493524/1‑143 (MQ=255) ccgACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAACCATGCCAATCTGGCGGCGCAGAAACGGAACTTCa > 2:124727/1‑143 (MQ=255) ccGGCGACGAATATCGTCACCGCAGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACGGTTCGGTCCCTGAGTAGATGGTGATCCTGGAAAATCATGCCAATAACGCGTCGCGGAAACGCAACATCACGGtata > 2:817152/1‑140 (MQ=255) gccacgaATACCATCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCa < 1:629451/140‑1 (MQ=255) gTCACCGCTGGCACCGGCGATAATCAACGGGATCGCCACGGGATCGTCGACAGTACGGCCCATCAGTAGATGGTGATCCTGGAAAATCATGCAAATCTAGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGt > 1:35781/1‑143 (MQ=255) cGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc < 2:26121/143‑1 (MQ=255) cGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGCACGGTCCATCCGTAGATGGTGATCCTGGAAAATCATACCAATCTGGCGGCGCAGAAACGGGACTTCACGGTTGCTCAGACGCCTGCGGTCATGGCCGcgcaac > 2:115740/1‑138 (MQ=255) cgtgaaTCAGCGGGATCCTCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAACTCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAg < 2:650301/139‑1 (MQ=255) tCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTGCACGGGTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTg > 1:689594/1‑143 (MQ=255) cGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGc > 1:48643/1‑143 (MQ=255) gTAGATGGTGATCCTGGAAAATCATGGCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTTCTGTCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggagg < 1:518800/143‑1 (MQ=255) tAGATGGTGATCCTGGAAACCCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAggg < 1:124727/143‑1 (MQ=255) tAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTGTTACAGACGCGTGATGGCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTGAATCCCACAGAACAACAACAGGAggg > 2:435631/1‑143 (MQ=255) tAGATGGTGATCCTGGAAAACCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAggg < 1:493524/143‑1 (MQ=255) tGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCCCTCAATCCCACACATCAGCTTCAgggggggcct > 1:811078/1‑139 (MQ=255) gATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTc < 1:382254/143‑1 (MQ=255) aTCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGTGTGATGTCATGGCCGCTAAACGAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTcc < 1:625485/143‑1 (MQ=255) tGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATACCCCCTCAACCAGTTTTTGGCACAACTTGGGCTCTCAATGCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTgc > 1:153893/1‑143 (MQ=255) tgagaaTCATGCCAATCTGGCGGCGCGGAAACGGAACTTCACGGTTTTGCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTgcg < 2:387451/139‑1 (MQ=255) | GCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCG > NC_000913/3602356‑3602634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |