| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 705,916 | T→C | 12.7% | intergenic (+26/‑177) | nagE → / → glnS | N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 705,916 | 0 | T | C | 12.7% | 94.1 / 5.3 | 44 | intergenic (+26/‑177) | nagE/glnS | N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (19/19); new base C (4/2); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01 | |||||||||||
CGCTCGATGATTAGCCCGGTGGTTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGC > NC_000913/705774‑706058 | cGCTCGATGATTAGCCCGGTAGTTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCtt < 1:51938/143‑1 (MQ=255) atCGATGATGAGGCCGGTGGTGTGCAGAAAAATCGAAGAAAGCAGCGGCTTGAACATAAAAGCACAAAGAAAAATTGTGGCGGGTCAAACATCGAAAAAAAAAAAAAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGa < 2:348866/142‑1 (MQ=255) gatgatTAGCCCGGTGGTTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCAACGCTTGAGCg > 1:503714/1‑143 (MQ=255) aGCCCGGTGGTTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTCAAGCGTCGCATc < 2:340606/143‑1 (MQ=255) gtggtTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCcaacaa < 1:782813/143‑1 (MQ=255) caATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGt < 1:530600/143‑1 (MQ=255) atCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGgag < 1:674628/143‑1 (MQ=255) atCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGgag < 1:323677/143‑1 (MQ=255) tCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGCGCGTCGCGTCCAACACTGCCAAGCGGCGggga > 1:353660/1‑143 (MQ=255) tCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCCACAAGGACAAGCGGTGgaga > 1:135067/1‑143 (MQ=255) gaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGACAAAAACCGCTGTAAGAAATAAAAAAGTAATCTGCATTATGCCTGATGCGACACATGAACGACGCAAACAACAATAACCAGCGGTAGAAAacttc > 2:235320/1‑143 (MQ=255) tttCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTtctc > 1:486096/1‑143 (MQ=255) ttCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTctct > 1:759514/1‑143 (MQ=255) ttGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCtttt > 1:489065/1‑143 (MQ=255) aTCATTAAAGCTCAGCGCCATATTGTGGCGGGGCAAACACCGCTGTATGAAATCAAAAAGTACTCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGctttcttt < 1:795930/143‑1 (MQ=255) tAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTtcat > 1:545783/1‑143 (MQ=255) gCTCAGGGCCCTATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCatcaat < 1:306218/143‑1 (MQ=255) tCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGGCGCTTTTTTTTTCatcaatga > 1:70541/1‑141 (MQ=255) cAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAatcat > 1:748666/1‑143 (MQ=255) tgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTGATCAATCATACCCACAACCCtt > 2:349608/1‑143 (MQ=255) tgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCtt > 1:5900/1‑143 (MQ=255) gggTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATGTTCTCTGCCGCTCTTTTTTTCATCAATCATCCCCATAATACTTGtca > 2:726570/1‑141 (MQ=255) gggTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCATGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTa < 1:616126/143‑1 (MQ=255) ggTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTGGTTAg > 1:55/1‑143 (MQ=255) gTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGCGATCTTCTCTGCCGCTTTTTTTTCCATCGATCATCCCCATAATCCTTGTTCGa > 1:353736/1‑143 (MQ=255) aaaCACGGCTGTGTGAAATCAAAAAGTGATCTGTTTTAGGGCTGAGGCGACGCTCGACTGTCGCATCCAACAATGTCAAGCTGTGTAGATTTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTa < 2:266640/143‑1 (MQ=255) aaaCACCGCTGTATGAAATCAAAAAGTAATCTGCGGTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTa < 1:437649/143‑1 (MQ=255) ccGTTGTGTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAAt < 2:415049/143‑1 (MQ=255) gccgtgtGAAATAAAAAAGTGATCTGCGTTAGGCCTGATGCGACGCGTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAAttt < 1:217185/137‑1 (MQ=255) cTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGGTTATCAAtttt > 1:519902/1‑143 (MQ=255) aTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAGTAAAcga > 2:705935/1‑141 (MQ=255) tCAAAAAGTAATCTGCTTTATGGCGGATGCGACGCGTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTaa < 2:759514/143‑1 (MQ=255) tCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGCTTATCAATTTTACAAAACTaa > 1:247335/1‑143 (MQ=255) aaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGtt < 2:5900/143‑1 (MQ=255) aGTACTCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCACCCAACAATGACAAGCGGTTGATATCTTCTCTGCCGCTTTTTTTTTCATCCATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGGTg < 2:790094/143‑1 (MQ=255) tAATGTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTc < 2:387448/143‑1 (MQ=255) attgttggATGCGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGAGCCCTTCTCTGCCGCTTTTTTTTTCCTCAATAATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTTTCAGCCTGTccc > 1:40373/8‑143 (MQ=255) attgttggATGCGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCCTGTTATATTGTCAATTTCAACTTCCTATCAGATGTCAGCATGTgcc > 1:733999/8‑143 (MQ=255) tGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTGGATTATCAATTTTAAAAAACTAACAGATGTCAGCCTGTccccc > 1:635523/1‑141 (MQ=255) cTGGTGCGACGCTTGGGCGTCGCATCCAACGATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTta < 1:548864/143‑1 (MQ=255) gATGCGACGCTTGAGCGTCGCATACAACTATGACATGCGGGGGCGTTCTTCGTGGCGGCTTTTTTTATCATCAATCCGCCCCATAATCCTTGTTAGATTAGCAATATTAAAAAACTAACAGGTGTCAGCCTGTCCCGCTtata < 2:249126/143‑1 (MQ=255) aTGCGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCTTCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTACAAAACTTCCAGTTGTCAGCCTGTCCCGCTTTTaa > 2:109888/1‑143 (MQ=255) aTGCGACGCTCAAGCGTAGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATGATCCACCTAATACTTGTTAGATTATCAATTTTAAAACACTAACAGTTGTCAGGCTGTCCCGCTGATaa > 1:545479/1‑143 (MQ=255) tGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCCTCCCCATAATCCGTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAg < 2:698935/143‑1 (MQ=255) cGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCAt < 2:543836/143‑1 (MQ=255) tGAGCGGCGCATCCAACAATGACCAGCGGTGGAGATCTTCTCCGCCGCTTTTTTTTTCATCAATCATCCCCATAAGCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGc < 2:217196/143‑1 (MQ=255) | CGCTCGATGATTAGCCCGGTGGTTTGCAGCAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGC > NC_000913/705774‑706058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |