Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,174,083	C→T	51.1%	R315H (CGC→CAC)	coaA ←	pantothenate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,083	0	C	T	51.1%	‑2.2 / 50.9	45	R315H (CGC→CAC)	coaA	pantothenate kinase
Reads supporting (aligned to +/- strand):  ref base C (11/11);  new base T (12/11);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTT  >  NC_000913/4173944‑4174224                                                                                                                                            |                                                                                                                                              tttCACGCGGAATAGACAAACAGGGGGCTTTATTCCGTGAGCGGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTa                                                                                                                                            >  1:228055/1‑143 (MQ=255)       gcgGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCCGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGa                                                                                                                                      <  1:428488/143‑1 (MQ=255)          gAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAGTTATTTGTGTAGTCTGACct                                                                                                                                   >  1:246280/1‑143 (MQ=255)            aTAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACctct                                                                                                                                 <  1:245122/143‑1 (MQ=255)             tAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCtctt                                                                                                                                >  1:791369/1‑143 (MQ=255)                         gggCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGAt                                                                                                                    >  1:70686/1‑143 (MQ=255)                         gggCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGAt                                                                                                                    >  1:511872/1‑143 (MQ=255)                                 ttaCTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACtt                                                                                                            >  1:632792/1‑143 (MQ=255)                                   aCTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACtttt                                                                                                          >  1:682313/1‑143 (MQ=255)                                      tggctgggatggatTACTGAACGCGTGGTTGGTCGGTGATATATCCCTGCGTAGTGCAGACAAATAAGAAAGGTGAGTATTCGCTCCGCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGt                                                                                                       <  2:221165/137‑1 (MQ=255)                                         gCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACATCTTCTACCGCATGATTAGCACTTTTCGTCAg                                                                                                    >  2:265059/1‑143 (MQ=255)                                           aGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGGCCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGa                                                                                                  <  2:109723/143‑1 (MQ=255)                                              aTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTCCCGCCTGATTAGCACTTTTCGTCAGGCTTa                                                                                               >  2:77194/1‑143 (MQ=255)                                                gggataataAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTaaa                                                                                             <  1:435404/140‑1 (MQ=255)                                                   ataagaAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATAATTAGCACTTTTCGTCAGGATTAAACTg                                                                                          <  1:354987/143‑1 (MQ=255)                                                    taataaAACCCTGGATGGGCGGTGAAAAAACCCGTCTTAGTTCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTgg                                                                                         >  1:348612/1‑143 (MQ=255)                                                      ataaAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATAATTAGCACTTTTCGTCAGGATTAAACTGgcg                                                                                       >  1:463927/1‑143 (MQ=255)                                                                      gCGGTGACATATCCCTGCGGCGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                                                       <  2:495940/143‑1 (MQ=255)                                                                      gCGGTGAAATACCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGCGGATGCGCTCCCCTGCAAATTCTTGGCGTAGTCTGACCTCTTCTATCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                                                       <  1:561144/143‑1 (MQ=255)                                                                            aaaTATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGCAGAatat                                                                 >  2:164027/1‑143 (MQ=255)                                                                            aaaTATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATAATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGCatat                                                                 >  1:664567/1‑143 (MQ=255)                                                                                 tCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGc                                                            >  1:353950/1‑143 (MQ=255)                                                                                  cccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCt                                                           <  2:70686/143‑1 (MQ=255)                                                                                            tGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGCTTAAACTGGCCCGCTCACGGGTCGGTCGAATATTTTGCGTTATGTTCAg                                                 >  2:41609/1‑143 (MQ=255)                                                                                             gCAGAAAGATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCGCTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGc                                                <  2:620053/143‑1 (MQ=255)                                                                                                 aaaaaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGt                                            >  1:619600/1‑143 (MQ=255)                                                                                                    aaTAAGAAAGGGGAGTATTCGCTGCCCTGGAAATTATTTGCGTAGTCTGGCCTCTTCTACCGCATGATTAGCACTTGTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGa                                         <  2:72356/143‑1 (MQ=255)                                                                                                     aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAt                                        <  1:750128/143‑1 (MQ=255)                                                                                                      tAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtc                                       <  1:673328/143‑1 (MQ=255)                                                                                                       aaGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtct                                      <  2:619600/143‑1 (MQ=255)                                                                                                       aaGAAAGGGGAGTATGCGCTCCCCTGCAAATGATGTGCGTAGTCTGACCTCCTCTACCGCATGATTAGCACTTGTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtct                                      <  1:44968/143‑1 (MQ=255)                                                                                                        aGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGCAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTAGAtctc                                     >  2:326274/1‑143 (MQ=255)                                                                                                           aaGGGGAGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCttt                                  <  1:742378/143‑1 (MQ=255)                                                                                                             ggggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAGACTGGCGCGCTCACGCGCAGGTAGAATATTTTGCTTTAAGTTCGGCCAGGTGACCTCTTTcc                                >  2:245699/1‑143 (MQ=255)                                                                                                              gggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAg                                             <  2:281704/129‑1 (MQ=255)                                                                                                              gggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAg                                             >  1:281704/1‑129 (MQ=255)                                                                                                                 aGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACaa                            <  2:52707/143‑1 (MQ=255)                                                                                                                     ttCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTACGTTCAGCCAGTTGATCTCTTTCCACAATGTc                        >  1:158232/1‑143 (MQ=255)                                                                                                                        gCTCCCCGGCAAATTATTTGTGTAGGCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTcgtt                     <  2:334379/143‑4 (MQ=255)                                                                                                                         cTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATgg                    <  2:682313/143‑1 (MQ=255)                                                                                                                           ccccTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAGACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCa                  <  2:205211/143‑1 (MQ=255)                                                                                                                             ccTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGt                >  2:807377/1‑143 (MQ=255)                                                                                                                               tGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCt              <  2:595129/143‑1 (MQ=255)                                                                                                                                           gCGTAGTCTGACCTCTTCTACCGCATGATTAGGACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTGGGTAAAATATTTGCCTGGATGGTCTGGCAGTTGATGTCTTTCCACAGTCTCATGTCGGTGTGAATCGcttctg  >  1:602021/1‑142 (MQ=255)                                                                                                                                           gCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTCGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTCTCCACACTGTCATGGCAGTCTTAATCGcttctt  >  2:731875/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                              TTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTT  >  NC_000913/4173944‑4174224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑