| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,147,548 | T→G | 10.7% | intergenic (+8/‑73) | rpmF → / → plsX | 50S ribosomal subunit protein L32/putative phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,147,548 | 0 | T | G | 10.7% | 94.9 / 2.9 | 45 | intergenic (+8/‑73) | rpmF/plsX | 50S ribosomal subunit protein L32/putative phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (18/21); new base G (3/3); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 | |||||||||||
GCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCA > NC_000913/1147407‑1147689 | gCATGCGTCGTTCCCATGACGCGCCGACCTCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCCAGGTCGTCGCCACGGAATCACGCATc > 2:722185/1‑143 (MQ=255) tcgtTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTTCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtga < 1:339213/143‑1 (MQ=255) cgtTCCCATGCCGCGCTGACCGGAGTCACCCGCCTGTCTGTAGACAAACCTTCTGGTGCAGAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGAGCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgat < 2:497713/143‑1 (MQ=255) aTGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCtt < 1:283402/143‑1 (MQ=255) tGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTa > 1:152252/1‑143 (MQ=255) gACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCGCGCATCTGCGTGATGAAGCTTAg > 1:127696/1‑143 (MQ=255) cTGACCGGAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAACCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGa < 1:24786/143‑1 (MQ=255) gagtgcccAGCCTGTCGTGAGCCAAAACTTCTGGTGAAAAACACCTGCGCCACCACATCACTGCCGACGGTTACGGCCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGGGATGAAGCTTAGTGAGGATTTTccc < 2:249827/137‑1 (MQ=255) gTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTCGTGAGGATTTTCCCCa > 1:636533/1‑143 (MQ=255) tCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACCTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGACGCTTAGTGAGGATTTTcccccc > 1:50722/1‑141 (MQ=255) tgtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTggg > 1:551329/1‑143 (MQ=255) gtAGACAAAGCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCGAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAg < 2:158986/143‑1 (MQ=255) gtAGACAAAACTTCTGGTGAAAAACCCCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAg < 1:65982/143‑1 (MQ=255) tAGACAAAACTTCTGGTGAAAAACACCTGCGGCACCACAGCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGCTGCGTGGTGAAGCTTAGTTAGGATTTTCCCCAGGCAACTGGGGAAAGa < 2:453893/143‑1 (MQ=255) ggACAAAACTTCTGGGGAAAAACACCCGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGAc < 2:350993/142‑1 (MQ=255) aGACAAAACTTCTGGTGAAACACCCCTGCGTCACCACATCACTGCCGACGGTTACTCCCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGAc < 1:656910/143‑1 (MQ=255) gACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGAcc < 2:254847/143‑1 (MQ=255) aCAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCa > 1:287312/1‑143 (MQ=255) aCTTCTGGTGAAAACCGCCTGCGTCACCACATCCCTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCg < 1:291677/143‑1 (MQ=255) tCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggc < 2:773613/143‑1 (MQ=255) gcgggTGAAAAACACTTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggc < 2:748758/140‑1 (MQ=255) tGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGGAAGCCCAAACCGGGCGGcga > 1:824753/1‑143 (MQ=255) tGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcga > 1:459641/1‑143 (MQ=255) gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTCGTGAGGATTTTCCCCAGGCAACTGGCGAAAGACCAAACCAGgcggcgcc > 2:438972/1‑141 (MQ=255) aaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgacggt > 2:449675/1‑141 (MQ=255) ccTGCGTCCCCGCACCCTCGCCGCCTGTTACAACCGCGGCCCCAAGGTCATCTCCAAGTAATCACGCATCTGCGTGATCAACCCTACTGAGGATTTTCCCCCAGAAAATTAGGAATGACTAAACCGCGCGGCGACCATACCtt < 2:292127/143‑1 (MQ=255) tCACCACATCACCGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACg < 1:649515/143‑1 (MQ=255) aCTTCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCGGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg < 2:23432/143‑1 (MQ=255) ccGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGt > 1:65091/1‑143 (MQ=255) ggcGGTTACGACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTa < 1:185551/141‑1 (MQ=255) gACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAAGCGGGCGGCGACGATACCGTGACCCGGCTAACCCTGGCGtga > 2:29910/1‑141 (MQ=255) gACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTa < 1:125472/143‑1 (MQ=255) aCGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAg < 2:287312/143‑1 (MQ=255) ggTTCCTGCCGCGGCCGCAAGGTCAGCGCTAGGTAAGCACGCGTCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGAt < 2:28456/143‑1 (MQ=255) ttagttccGTGGCTGCGAGGTCCTCGCTAAGTAATCACGCATCTGCGTGTGGAAGCTGGGTGATGATTTTCCCTAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGt < 2:622901/137‑1 (MQ=255) ctacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGACGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGAAGATACCTTGACACGTCTAACCCTGGCGTTAGATGTcct > 2:578311/1‑141 (MQ=255) ccGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGa > 1:447886/1‑143 (MQ=255) ccGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGa > 1:189172/1‑143 (MQ=255) gCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAtt > 1:740237/1‑143 (MQ=255) aaGGTCATCGCTAAGTAATCACGCATCTGCGGGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAtttt < 2:160000/143‑1 (MQ=255) tAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGt < 2:152252/143‑1 (MQ=255) cttcaTCACGCAGATGCGTGATTAAGCTTAGTGAGGATTTTCCCCAGGCAACTGTGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGa > 1:760094/5‑143 (MQ=255) cttcaTCACGCAGATGCGTGATTAAGCTTAGTGAGGATTTTCCCCAGGCAACTGTGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCGTCCGTGa > 1:439327/5‑143 (MQ=255) tcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACa > 1:442424/3‑143 (MQ=255) aTCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGAAGATACCTGGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGtgcatgca > 1:517285/1‑143 (MQ=255) | GCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCA > NC_000913/1147407‑1147689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |