| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,472,437 | T→C | 7.4% | N33D (AAT→GAT) | pyrL ← | pyrBI operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,437 | 0 | T | C | 7.4% | 185.9 / 5.2 | 80 | N33D (AAT→GAT) | pyrL | pyrBI operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (37/33); new base C (4/2); total (41/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01 | |||||||||||
TTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCA > NC_000913/4472295‑4472574 | tttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGAtt > 2:243307/1‑143 (MQ=255) tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTGTCTCCTGACGCCTGCGCAAAAAAA‑AGCCCCTCGATTGAggg > 1:639299/1‑143 (MQ=255) tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTCTCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGAGTGAgcg > 1:695636/1‑141 (MQ=255) tgaGTTTAGCCGCTGGCGCCCGCACACGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTATGATATAGCGGATTAGG‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCAATCGAGGGGc < 1:587241/141‑1 (MQ=255) tCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAGGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCGAAAAAA‑AGCCCCTCGATTGAGGGGc > 2:651893/1‑143 (MQ=255) tCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCGCTCGATTGAGGGGc > 1:145688/1‑143 (MQ=255) caggtgcgccgcTGACGCCAGCACCAGAGTAAGGTCATCGCGACTAAGGGCGAATATGGAAATGATATGTTTCTGATATAGCAGATTAGC‑CATCTTTTATCTCCTGAAAAAAAAAAAAAAAAA‑AGCCCCTCAATCGAGGGGCt < 2:395018/137‑1 (MQ=255) atgttCGCCGCTGTCGCCAGCCCCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCCGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTg < 1:52945/140‑1 (MQ=255) gTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGAGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTgg > 1:370038/1‑143 (MQ=255) tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGGCTAAGGACGTTTATGGAAATGATATGTTTCTGAAAGAGCGGAGTAGC‑CAGCGATTATCTCCTAACGCGTGGGCAGAAAAA‑AGCGCCTCGGTTGAgggggtagg > 2:209095/1‑138 (MQ=255) ccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGACTgg > 1:459257/1‑143 (MQ=255) ccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGAAATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCGAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATgg > 1:517127/1‑143 (MQ=255) gcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGt > 1:541460/1‑143 (MQ=255) tgtggacAGCACTAGGATAAGGTTATCTGTGCTAAGTTAGTATGTGGAAATGATATGTTTCTGATATAGGGGATTAGT‑CATCTTTTATTTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGa < 2:800912/137‑1 (MQ=255) cgccAGACTAAAGTCAGCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCATGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGga < 2:368361/141‑1 (MQ=255) aCCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTGCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaa < 2:270095/143‑1 (MQ=255) cAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaaga > 1:298309/1‑143 (MQ=255) ttAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa > 2:679100/1‑143 (MQ=255) ttAAGGTCATCGCGACTAAGGTCGTTTATGGAAAGGATATGTGTCTGATATAGCGGATTAGCGCAT‑ATAGAAGTTCTTACGGCTGGGCCAATAAA‑ATCCCCTCTATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa < 1:381443/143‑1 (MQ=255) aTCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑TATCCTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGc < 1:735352/143‑1 (MQ=255) gcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGAGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGGGATCAACGAGAGGAACAACAGCAGGCCAg > 2:480657/1‑143 (MQ=255) ctacgatggTCGCTTTCGGAAATGATATGTTTCGGATACAGCGGATTCGC‑CGTCTTTTATCTCCTGACGACCGGGCACAACAA‑GGCCGCTCGATTGTGGGGCTCGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCAAGc < 2:209783/136‑1 (MQ=255) gACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCg > 1:800192/1‑143 (MQ=255) aCTAAGGTCGGTGATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAAGGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGt < 1:43774/143‑1 (MQ=255) tAAGGTCGTTTATGGAAATGATATGTTTCTGATACAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCGAAAAAA‑AGCCCCTCGATTGCGGGGCTTGGTATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCt > 1:305135/1‑143 (MQ=255) atgtagTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCTTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttt < 2:157647/138‑1 (MQ=255) ttagTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGACAAAGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttttt < 1:782458/140‑1 (MQ=255) gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGACAAAAAAA‑AGCCCCTCGATTGAGGGTCCGGGAATGGGTGGTCGACGGGAAGAAAACCGGCAGGCCCGCGTCttttt > 1:755384/1‑143 (MQ=255) tCGTGTATGCAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCtttttt < 2:517127/143‑1 (MQ=255) cGTTTATGGACATGATATGTTGCTGATATAGCGGATTAGG‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc < 2:262757/143‑1 (MQ=255) ttATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGa < 1:408238/143‑1 (MQ=255) gAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATGTCTTGACGACTGAGCAAAAAAACACCGCATTCAGCG‑TGGGCTGGGAATGGGTGATCCACGGGAAGACAAACGGCAGGCCAGCGTCTTTTTTCCGACGCgg > 1:190695/1‑143 (MQ=255) gAAATGATATGTTTCTGATATAGCGCGTTAGC‑CATCTTGTATCTCCTGAAGCCTGGGCAAAAAAA‑AAACCGTGTGATGAGTTGCTGGGAATGGGGGAGCAACGGGAAGAAAAACGGCAGGCGAGCGGCGTTTTTCAGACGCgg > 2:584725/1‑143 (MQ=255) atatGTTTCTGATATCGCGGATTAGC‑CATCTTCCATCGCCGGACGCCTGGGCAAAAAAA‑AGCCCCTCGATGGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGAc < 1:163508/143‑1 (MQ=255) atGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGACTGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGAGGTAAGCCaa > 2:158989/1‑143 (MQ=255) gTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTGTTTTCAGCCGCGGTAAGCCaaaa > 2:5332/1‑143 (MQ=255) gTTGCTGATGTAGGTGATTGGC‑CATCTTGTATCGCCGGACGCCTGGGCAAAAAAT‑AGCCCCTCGATGGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaaa < 2:421696/143‑1 (MQ=255) tGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg < 1:115578/143‑1 (MQ=255) ataGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTCTCAGACGCGGTAAGACAAAATGTGGAacg > 2:234050/1‑142 (MQ=255) ataGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGCCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca < 1:367308/143‑1 (MQ=255) aGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGTGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca < 1:407604/143‑1 (MQ=255) aGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca < 2:541460/143‑1 (MQ=255) gCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGGATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACACAATGTCGAacacac > 2:682482/1‑143 (MQ=255) gCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCCAacacac > 1:266194/1‑143 (MQ=255) cGGCTTAGC‑CATCGTTGATCGCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACt < 1:510683/143‑1 (MQ=255) gATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACACAATGTCGAAAACACTGa > 2:33968/1‑143 (MQ=255) tAGC‑CATCTTTTGTCTCCGGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAcc < 1:106101/143‑1 (MQ=255) gC‑CATCTTGTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGCGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt < 2:267477/143‑1 (MQ=255) c‑cATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa < 1:325305/143‑1 (MQ=255) c‑cATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGAGTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa < 1:385398/143‑1 (MQ=255) c‑cATCTTTTATCTCCTGAAGCCTGGGCAAAAAAA‑AGCGCTTCGATTGAGGCACTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAAGCGGTAAGACAAAAATTCGAACACACAGAACCATa > 2:175588/1‑143 (MQ=255) c‑cATCTTCTCTCCCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGAGTGGGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa < 1:41588/143‑1 (MQ=255) c‑cATCCTTTATCTCGTGACGCCTGGGCAAAAAAA‑CGCCCGTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa < 2:248699/143‑1 (MQ=255) cATCTTTTATCTCCTGACGCCTGGGGAAGAAAA‑GGCGGCTTGAGTGAGGGGGTGGGAATGGGTGATCAAGGGGGAGAGAAACGGCAGGCGAGCGTCTTTTTTCAGGCGCGGTAAGAGAGAATGTCGAGCGCACTGAACCATAc > 1:806431/1‑143 (MQ=255) aTCTTTTATCTCCTGACGCCTGGGCAAAAACA‑ATACCTGAAGTTGAGGGGCTGGGGATGGAAGAAGAACAGAAATTAATACGGCATTCCATCGTCAACATTCAGATGCGGAAAGACAAAAGGTCGAACACACTTAACGATACa > 2:290676/1‑143 (MQ=255) aTCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAGATGTCGAACCCACTGAACCATACa > 1:502590/1‑143 (MQ=255) aTCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAGCATACa > 2:1454/1‑143 (MQ=255) tCTTTTATCTCCTGACGCCTCGGC‑AAAAAA‑AGCCCCCCGATTGCGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtc < 1:295333/143‑1 (MQ=255) ttttATCTCCTGACGCCTGGGCAAAACAA‑AGCCCCTCGATTGCGGGGCTGGGAATGGGTGATCAACGGGAATAAAAACGACAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcc > 2:786234/1‑143 (MQ=255) tATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcctcc < 1:554873/143‑1 (MQ=255) atgcccggACGCGTGGGCAAAAAAA‑AGCCCCACGATGGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTccc < 1:238327/136‑1 (MQ=255) tcCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACGCACTGAACCATACATCCTCCCGGc > 1:200420/1‑143 (MQ=255) aCGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAAtt > 2:603151/1‑143 (MQ=255) aCGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAAtt < 1:225396/143‑1 (MQ=255) aCGCCCGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAAtt < 1:243307/143‑1 (MQ=255) gCCTGGGCAAAAAAA‑ATCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCCGCGTCTTTTTTCAGACGCGGTAAGACAAACTTTCGAACACACTGAACCATACATCCTCCCGGCAAATTGt < 2:806564/143‑1 (MQ=255) gCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGt > 2:160147/1‑143 (MQ=255) tgtgCAGAAAAG‑AGCCCCTGGGTGGAGGGCCTGGGAATGGGTGGGCAAGGGGAAGAAAAACGGCAGGCCAGCGTCTTTGTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCg < 2:462536/140‑1 (MQ=255) ggCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAAGGGGTGATCAAGGGGAAGAAAAACGGCAGTCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGc > 1:367313/1‑143 (MQ=255) gCAAAAAAG‑CGCCCCTCGAGTGAGGGGCAGACCGTGATATACCAACGGTAAGAAAAGCGGCGGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa < 2:169385/143‑1 (MQ=255) gCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGACCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCAGCAAATTATCCGGCa > 1:585185/1‑143 (MQ=255) caaaaaa‑aGCCCCTCGATTGAGGGGCTGGGCATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTCCTCCTTTTGTTTTGTGCAGAAATGTCGAGCACACTGAACCAAACATCCACCCCGCAACTTGTACGGCCtt > 2:202516/2‑143 (MQ=255) aaaaaa‑aGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCCttg > 1:180998/1‑142 (MQ=255) aaaaa‑aGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATtat > 2:417181/1‑143 (MQ=255) aaaa‑aGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAAGAGAATAGACTTAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATtata < 1:420704/143‑1 (MQ=255) ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACCCTGAACCATACCTCCTCCCGGCAACTCGTCCGGCATTATACt > 2:182967/3‑143 (MQ=255) caGCCCCTCAATCGAGGGGCTGGGAAGGGGTGAGCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTc > 1:176398/2‑143 (MQ=255) ccccTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGACCCATACATCCTCCAGGCAGATTGGCCGGCATGATACTCATc > 2:692562/1‑143 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGCCAAACGCCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACGAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTc < 2:800192/143‑1 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGAGTCTTTTTTTAGACGCGGTAAGACAAAATGTAGAAGACACTGAACCATAATTCCTCCCGGTAAATTGTCGGGCATTATACTCATCGTc > 2:286113/1‑143 (MQ=255) tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCCGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa < 2:535739/143‑1 (MQ=255) | TTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGC‑CATCTTTTATCTCCTGACGCCTGGGCAAAAAAA‑AGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCA > NC_000913/4472295‑4472574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |