| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,260,901 | C→T | 13.8% | intergenic (‑98/+27) | dauA ← / ← prs | aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,260,901 | 0 | C | T | 13.8% | 59.1 / 3.3 | 29 | intergenic (‑98/+27) | dauA/prs | aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (13/12); new base T (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTC > NC_000913/1260760‑1261038 | agagCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATCACAGACAAAAAAACccgccg > 2:400096/1‑143 (MQ=255) cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGACATTAGAGCCATAAAAACCCGCCGCGGc > 1:337968/1‑143 (MQ=255) aaaGGCGTCGCATGTGAGGAAGATATTTTGTTCACAATAATGTTTCAGCCGTGAGCGGATCAGCT‑GTCAACTAAATGGCACGTGAAGGAGGCAGAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCTGCGGCGGGtt < 2:399607/143‑2 (MQ=255) tCACATGTGAGGAAAATATTTTGTTCCCAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCTGCGGCGGGTttttttgt < 1:581189/143‑5 (MQ=255) acaTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCGTAGGTCATACAAATGGATATTACAGAGAAAAAGACACGCCGCAGCGGGTCTTTGAGCCg > 1:493081/1‑143 (MQ=255) gAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GCCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACACGCCGCAGCGGGTCTGTCAGCCGGGTTCGGtt > 2:219281/1‑143 (MQ=255) atTTTGCTCACAATAATCGTTCATCCGCGAGCGCATCAGCTCGCCAAACCCA‑GGCAGCGGAGGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGtt < 1:599634/143‑1 (MQ=255) tttGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAAGTCATTCAAATGGATACTACCGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAATAGCGTTCg > 2:583088/1‑143 (MQ=255) tGTTCACAATAATGTTGCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGaa < 2:56039/143‑1 (MQ=255) acaATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGGGTTCGAACATgg > 1:632659/1‑143 (MQ=255) gaatCTTTCATCCGTGCGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCaga < 2:464070/142‑1 (MQ=255) ccGGGAGCCCATCATCT‑GTCAACTAAATGCCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAACACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt < 1:731928/143‑1 (MQ=255) gcATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTg < 1:44422/143‑1 (MQ=255) aCTAAATGGCAGGTGAAGGAGGCATAGGTCATACACATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg < 1:417342/143‑1 (MQ=255) cgaaaGGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGc < 2:22198/141‑1 (MQ=255) aaTGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtgc > 2:730686/1‑141 (MQ=255) cAGGGGAAGGAGGCATAGGTCATGCAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCa < 1:630905/143‑1 (MQ=255) cAGGCGAGGGAGGCATCGGTCAGACAACGGGATATCACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATCAGTGTTCGAACATGGCAGAGTTCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCa < 1:51338/143‑1 (MQ=255) agggTGAAGGGGGCATAGGTCATACAAAGGGATATTACAGACAAAAACCCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCa < 2:285164/141‑1 (MQ=255) ttCACACAAATGGACATTACAGCCAAAAACACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa < 1:47225/142‑1 (MQ=255) gTCATACAAATGGATATTACAGACAAAAAAACCCGCTGCAGCGGGTCTTTGAGCCGGGGTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa > 1:530872/1‑143 (MQ=255) gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGACCAGCATACATGACAGGGTCa > 1:240526/1‑143 (MQ=255) gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGGTCGATCAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa > 1:739768/1‑143 (MQ=255) aTACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCagag > 2:755664/1‑143 (MQ=255) aCAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCAGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCATCGGGCAGCATACCTGACAGGGTcgcagca > 2:564005/1‑137 (MQ=255) atatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATCCCTGACAGGCTCAGCGTACGCACGtc > 2:266684/1‑142 (MQ=255) tcaaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatt > 1:538257/7‑143 (MQ=255) aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatt > 1:264105/1‑143 (MQ=255) aaaaaaCCCGCCGCAGGGGGGCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgattt < 2:747092/143‑1 (MQ=255) cccGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGCGTACGCACGTTCGGCAGTGATTTGATTTc > 1:219621/1‑143 (MQ=255) | AGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCT‑GTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTC > NC_000913/1260760‑1261038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |