Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,934,900 C→T G472E (GGA→GAA)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,9000CT94.7% 102.9 / NA 38G472E (GGA→GAA) zwfNADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (18/18);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCA  >  NC_000913/1934760‑1935038
                                                                                                                                             |                                                                                                                                           
cTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTtcc                                                                                                                                           <  1:712043/143‑1 (MQ=255)
 tGAAAGTGTAAAAGTTGTGCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTtccc                                                                                                                                          <  1:76394/143‑1 (MQ=255)
   aaaGTGTAAAAATTGTTCTACAATCTGCGCAAGAGCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATCCCAGGAACGACCATCACGGGTAATCATCGCCACCGACCCACCGGGCCCCCac                                                                                                                                        >  2:306004/1‑142 (MQ=255)
        gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCCACGGGGTGCCGGGTTcc                                                                                                                                   >  1:320799/1‑143 (MQ=255)
         tcaagaTTGTTCTCCAATCTGCTCAAGATCATGTTACC‑GGTAAATTAACCATAAAGGATAAGCGCAGATATTACGCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCg                                                                                                                                  <  2:67839/138‑1 (MQ=255)
          aaaaaTGGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCgg                                                                                                                                 <  1:389045/143‑1 (MQ=255)
           aaaaTTGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGCATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTACTCAACGCCACCGAGTCACCGGGATCCCACGCACCGGc                                                                                                                                >  2:312164/1‑143 (MQ=255)
               ctGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCCTAACGGCTAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGa                                                                                                                            <  1:194419/142‑1 (MQ=255)
                tggtCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAGTCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGAt                                                                                                                           >  1:554978/4‑143 (MQ=255)
                     tACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGt                                                                                                                      >  1:749531/1‑143 (MQ=255)
                     tACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGt                                                                                                                      >  1:355251/1‑143 (MQ=255)
                      tgaagcTGCGGAAGATC‑TGTTTCCTGGTATAATTAGTATGAAGGATAAGCGCAGATATTACTCACACTCATTCCATGAACGACGATCACGTGTAATGATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGtt                                                                                                                     <  2:565960/138‑1 (MQ=255)
                         aTCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCg                                                                                                                  >  1:572127/1‑143 (MQ=255)
                              cgcAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGGTGCGGTTTCGGCGca                                                                                                             >  1:87097/1‑143 (MQ=255)
                                    aTCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGGTATTACTCAAACTCATTCCAGGAACGACCTTCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTg                                                                                                      <  2:394653/143‑1 (MQ=255)
                                     tcctGTTACG‑GGTAAAATAACGATAAAGGATATGCGCAGATATTGCTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATTCGGTTTCGGCGCATCA‑TTGt                                                                                                     <  2:763482/140‑1 (MQ=255)
                                     tCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGt                                                                                                     <  1:465638/143‑1 (MQ=255)
                                     tCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCACGTTCCGGCCTGCTACGGTTTCGGCGCATCA‑TTGt                                                                                                     >  1:658834/1‑143 (MQ=255)
                                                         aCCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCCTCGCCGACGCCTCAGt                                                                                  >  1:647539/1‑143 (MQ=255)
                                                         aCCACAAGGGAGACGCTCAGAGATTACTCAAGCTCATGCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGTAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGt                                                                                  <  1:361801/143‑1 (MQ=255)
                                                           cATAAAGGATAAGCGCAGATAGTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGGTCCCAGGTTCCGGCCTGATGCGGTTTCGGCGCAGCA‑TTGTCCATCGCCCACGCCTCAGTaa                                                                                >  1:843241/1‑143 (MQ=255)
                                                            aTAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAAt                                                                               <  2:749531/143‑1 (MQ=255)
                                                             tAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATg                                                                              >  1:625714/1‑143 (MQ=255)
                                                             tAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCCGATACGGTTTCGGCGCCTCA‑TTCTTCCCCGCCACCGCCTCAGTAAtc                                                                              >  1:115537/1‑142 (MQ=255)
                                                                          cAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTGGGCGCATCA‑CTGTCCATGGCCTACGCCTCAGTCATCGAGTGTGCCCAtt                                                                 >  1:352293/1‑143 (MQ=255)
                                                                              tatTACTCAAACTCATTCCAGGAACAACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa                                                             <  1:155236/143‑1 (MQ=255)
                                                                              tagtACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCGTCGGCGCCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa                                                             <  1:716476/140‑1 (MQ=255)
                                                                                   cTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCCCGTCTCCGTAATGGAGTCTACCCATTTCCAGGctt                                                        >  1:356530/1‑143 (MQ=255)
                                                                                     cAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTGATGGAGTCTCCCCCTTCTCAGtcttct                                                      >  1:130707/1‑143 (MQ=255)
                                                                                      aaaCTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttctt                                                     >  1:793441/1‑143 (MQ=255)
                                                                                                       gACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGt                                    <  1:658254/143‑1 (MQ=255)
                                                                                                          cATCACGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACa                                 <  1:377089/143‑1 (MQ=255)
                                                                                                              aCGGGTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACa                             >  1:585357/1‑143 (MQ=255)
                                                                                                                 ggTAATCATCGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTg                          <  1:676564/143‑1 (MQ=255)
                                                                                                                   gaaTCACCGCCACCGAGGCACCGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCAATTCCAGTCTTCTTCCACTTCGTCGCGACGTACAAACAGTGcc                        <  2:843241/142‑1 (MQ=255)
                                                                                                                      tcatcGCCACCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGCTTTACG‑GACTCAGCTCTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGa                     <  1:856082/143‑1 (MQ=255)
                                                                                                                          cGCCACCGCGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGCTCCCGCGCCTCA‑TCGTCCTTCGTGCACCACTCAGTCACTGCCTCGACCGATGTCCAGGCTTCTTCCAGTTCGTCGCGACGTACAAACAGTGCCTGAATAc                 <  2:381807/143‑1 (MQ=255)
                                                                                                                              aCCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACg             <  1:605358/143‑1 (MQ=255)
                                                                                                                              aCCGAGGCAACGGGTTCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACg             >  1:454332/1‑143 (MQ=255)
                                                                                                                                         gcgTCCTCATGTTCCGGCCTGATACGCTTTCGTCTCCTCA‑TTGTCCATCGCCCAAGCAACAGTAAGGGATTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCca  <  2:516198/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
CTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACC‑GGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCA‑TTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCA  >  NC_000913/1934760‑1935038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑