Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	69.7 / NA	25	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/13);  total (12/13)

GAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132657‑2132925                                                                                                                                   |                                                                                                                                           gagGGTAAACAGCTGATATTTTTTGCTGGAGCGTTCGTGGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGCCTCTGCGTCGCct                                                                                                                                >  2:523250/1‑143 (MQ=255)  agGGTAGACAGCTGGTATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                               <  2:857421/143‑1 (MQ=255)    ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtgtt                                                                                                                             >  1:292072/1‑143 (MQ=255)              tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAAGCGCGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   >  2:8115/1‑143 (MQ=255)               gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                                  >  1:819787/1‑143 (MQ=255)                          cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                       <  2:572653/143‑1 (MQ=255)                                 gttggAATTTGTCTTTGTCCTTGCGGTAAGAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                                <  1:750532/139‑1 (MQ=255)                                       gtttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGTATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                          <  1:823026/142‑1 (MQ=255)                                           gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTCCTCCACCACCATCCGCa                                                                                      >  2:268312/1‑143 (MQ=255)                                              tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCACGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCATCACCATCCGCAGGa                                                                                   >  2:424126/1‑143 (MQ=255)                                              tttGTGCGTGCGGTTAACGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                   <  2:573016/143‑1 (MQ=255)                                               ttGTGCTCGCGGTAAAAGTGGAACTCCCGCGAGTAATCATTCGCTGTTGTGTTTATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                  <  1:392741/143‑1 (MQ=255)                                                   gCTTGGGGTAAAAGGAGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                              <  1:777119/143‑1 (MQ=255)                                                           caaaaGTGGACGCACCCGGATACTTTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTCCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                      <  1:610660/142‑1 (MQ=255)                                                             aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTTCTCCACCACCCTCCGCAGGAAGATGTCGTAATGCt                                                                    >  2:853269/1‑143 (MQ=255)                                                                gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAAGCCtgtg                                                                 >  1:413407/1‑143 (MQ=255)                                                                gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                 <  1:289625/143‑1 (MQ=255)                                                                          ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACTCTGGTGTGTGTGAGGTCGTAACCCTGTGCCGCTTTCAg                                                       >  1:156311/1‑143 (MQ=255)                                                                                  ttttttCGGTGACGAGGTGCTCCGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                               <  1:301833/143‑1 (MQ=255)                                                                                        cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                         <  1:8115/143‑1 (MQ=255)                                                                                        cGGTGACGAGGCCATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                         <  1:354022/143‑1 (MQ=255)                                                                                          gTGACGAGGTGATCTGCATTTCACCGAGATTGATATGCAGGCTCTGCGTCGCCAGTGCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:98708/143‑1 (MQ=255)                                                                                             aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGcaccacccc                                    >  1:640094/1‑137 (MQ=255)                                                                                                                 ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACGACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGgccc                >  1:38767/1‑142 (MQ=255)                                                                                                                               cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACAATCCGTAGCTAGATTTCGTAATCATGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt  >  1:160613/1‑143 (MQ=255)                                                                                                                                   |                                                                                                                                           GAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132657‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑