| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,447,483 | G→T | intergenic (‑10/+25) | aroC ← / ← prmB | chorismate synthase/50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,447,483 | 0 | G | T | 96.9% | 86.7 / ‑6.0 | 33 | intergenic (‑10/+25) | aroC/prmB | chorismate synthase/50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (18/13); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
TCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAAC > NC_000913/2447343‑2447615 | tCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCttt > 2:189104/1‑143 (MQ=255) cGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGttt < 2:479660/143‑1 (MQ=255) cAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGCCCCATTGTGTTGCCTGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACt < 1:676422/143‑1 (MQ=255) gAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGACAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATAGTTGTGTTTGCGTGTTTACTTAATc > 2:576915/1‑143 (MQ=255) gAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATc > 1:217351/1‑143 (MQ=255) gAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGAGTATATAATAAACAGATCACCTTTATTGTTGTGTTTGCGTGTTTACTTAATc > 2:378168/1‑143 (MQ=255) tGCCTGGCCGAACACCAGCGACGATGCGGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCttt < 2:91223/143‑1 (MQ=255) ccTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGCTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTCCTTAATCTTtat > 2:356774/1‑143 (MQ=255) gCGGAACACCATCGACGATGCAGCCCAGCGCCAGCCCTTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAAt < 2:85053/143‑1 (MQ=255) cacCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGACTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCGCGGCTCCTTTATTGTGGTGTTTGCGTGTTCCGTTACTCATTTTAGATCGCGaa > 2:334794/1‑143 (MQ=255) acCATCGACGATGCAGCCGAGCGCCAGTCCGTGCGATTCGCCGTAGGTGGTTACGCGTAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCTTTTATTTTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGaaa < 2:703576/143‑1 (MQ=255) cATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTCCGCGAGAGAGTTGTCCAATTATGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGATTGCGTGTTTACTGAATCTTTATAATTCGCGAAATg > 1:240840/1‑143 (MQ=255) aGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGGGTTTGCGTGTGTACTTAATCTTTATAAATCGCGGAGTGTTCTCGTGCGGc > 1:464271/1‑143 (MQ=255) gAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGCGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTCATCTTTCTAAATCGCGACATGTTCTCGCTCGGCAATa > 1:16397/1‑143 (MQ=255) cAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGTTTTTTTTTTTTTGTGTTTGTGTGTTTACTTAAGCTTTATAAAGCGGGAAATGTTCTCGTGCGGCAATAAgctgc < 1:161889/143‑1 (MQ=255) taTTCGCCGAAGCTGGGTACGCGAAAGCGTTGTCCAAGTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGa < 2:442113/142‑1 (MQ=255) ttCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGc < 2:217351/143‑1 (MQ=255) cGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCAt < 1:189104/143‑1 (MQ=255) ccGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGCCTCGGGTATCGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAGATGTTGTCGTGCGGCAATAAGCTGCTCTTCGGCGAGcgcac > 2:864477/1‑139 (MQ=255) cGAAGGGGGTTACGCGAAAGAGTTGTGCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGGTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCGCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATaaa < 2:804582/143‑1 (MQ=255) gAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAac > 1:839595/1‑143 (MQ=255) gAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAac > 1:838340/1‑143 (MQ=255) cgAAAGAGTTGTCCAATTGTGTTCCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATcgccgc < 1:95249/143‑1 (MQ=255) ttGTCCAATTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATcc > 1:745043/1‑142 (MQ=255) gTCCAAGTGGGTTTCCAGCGATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCaaa < 2:240554/143‑1 (MQ=255) ccACTTGTGTTTCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGACATGTTCCCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACt < 1:773549/143‑1 (MQ=255) gtgtTTCCAGCCATCACGCCTCCCTCATTGTTGTGTTTGCGTGTTTACTTAATCTTTATCAATCGCGCAATGTTCTCGTGCGTCAACAACCTGCTCTTTGGTGAGCACAAACACACCATCGCCGCCGCGATCAAACCccagcc > 2:300119/1‑143 (MQ=255) tttCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTCCTTAATCTTTATAAATCGCGAAATGTTCTCGTCCGGCAATAAGCTGCTCTTTGGTGAGCATAAACCCCCCATCGCCGCCGTTCTCACACTCCACCCAgg > 1:723224/1‑143 (MQ=255) tttCCAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTCACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATACACACACCATCGCCGCCGCTACCAGACTCCCGCCAgg > 2:269451/1‑143 (MQ=255) cAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTCTCAAACTCCAGCCAGGTGaa > 1:697933/1‑143 (MQ=255) cAGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGaa > 1:497443/1‑143 (MQ=255) aGCCATCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGGGAAc > 1:594076/1‑143 (MQ=255) ccATCACGGCTCCTTTATTGTTAGGTGGGCGTGGTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACgg > 2:307398/1‑143 (MQ=255) tCACGGCTCCTTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATCTTCTCGTGGGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCGTGCAGGTGGACGGAAc > 1:55772/1‑143 (MQ=255) | TCATGTTGCAGGTCCGCTTCCGTCAGCGGAATGCCTGGCGGAACACCATCGACGATGCAGCCGAGCGCCAGCCCGTGCGATTCGCCGAAGGTGGTTACGCGAAAGAGTTGTCCAATTGTGTTTCCAGCCATCACGGCTCCGTTATTGTTGTGTTTGCGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAAC > NC_000913/2447343‑2447615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |