Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,816	T→G	T106P (ACC→CCC)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,816	0	T	G	100.0%	116.0 / NA	38	T106P (ACC→CCC)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/17);  total (21/17)

TGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCAC  >  NC_000913/2661676‑2661944                                                                                                                                             |                                                                                                                                 tgtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCa                                                                                                                                <  2:512449/143‑1 (MQ=255) tgtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCa                                                                                                                                >  2:718421/1‑143 (MQ=255)         tGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGGAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTa                                                                                                                        >  1:280806/1‑143 (MQ=255)                cgcgTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATGAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATcgccgc                                                                                                                 <  2:633007/143‑1 (MQ=255)                   gccgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCt                                                                                                              <  2:690829/141‑1 (MQ=255)                    tcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTACCGCCGCCCTg                                                                                                             >  2:410042/1‑143 (MQ=255)                      gtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTGAAGTAATGTTGTTGAGAAAACCGATGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCGTTTATCGCCGCCCTgga                                                                                                           >  2:548638/1‑142 (MQ=255)                            ctGCTGACGGCCAGACACATCCAGCACTTCGTGGGTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAgccgc                                                                                                     <  2:288020/142‑1 (MQ=255)                                tGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAACGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTACCACCGCCCTGGCAGCCGCCctt                                                                                                 >  2:424769/1‑143 (MQ=255)                                 gacgacCAGACACAGCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTa                                                                                                <  1:554165/143‑1 (MQ=255)                                 cgcgcgcAGACACATCCAGCATTTCCGGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTa                                                                                                <  1:483292/137‑1 (MQ=255)                                     accggccacaTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTGGAGAAAACCGGTGATACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCt                                                                                            <  2:146847/137‑1 (MQ=255)                                       cAGACACATCCAGCACTTCCTGGTTAGTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACTGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGCCAGCCGCCTTTAccgtca                                                                                          >  1:384350/1‑139 (MQ=255)                                          acacATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACaa                                                                                       >  2:160039/1‑143 (MQ=255)                                            acaTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCAGCCCTGGCAGCCGCCTTTACCCTGACAACg                                                                                     >  1:37965/1‑143 (MQ=255)                                            acaTCCAGCACTCCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGCCCACg                                                                                     >  1:730986/1‑143 (MQ=255)                                                  aGCACTTCCTGGTTAGTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGc                                                                               <  1:132909/143‑1 (MQ=255)                                                            ggTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGAt                                                                     >  1:274688/1‑143 (MQ=255)                                                               tattgACCAGTTCGCCTAAAGGAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCg                                                                  <  2:591115/143‑1 (MQ=255)                                                                 ttaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc                                                                >  1:170755/1‑143 (MQ=255)                                                                 ttaACCAGTTCGCCTAAAGTAATGTTGTTAAGAAAACCGGTGATACTGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc                                                                >  1:591920/1‑143 (MQ=255)                                                                  taACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTTGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCa                                                               >  1:487393/1‑143 (MQ=255)                                                                   aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACTCTGACAACGGGTGGCATCTACAGATTCGTCaa                                                              <  1:559344/143‑1 (MQ=255)                                                                   aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCaa                                                              >  1:469680/1‑143 (MQ=255)                                                                                            ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGc                                     <  2:274688/143‑1 (MQ=255)                                                                                            ttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGc                                     >  2:826082/1‑143 (MQ=255)                                                                                             tgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCCCCCTGGCAGCCGCCCTTACCCTGACAACGGGTGGCATCTACAGGTTCGTCAACGGCGCCAATTCCTTCGCCCACGGCg                                    >  2:151871/1‑143 (MQ=255)                                                                                                     ccGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTCCAGATTCGTCCACGGCGCTAATTACTTCGCCACCGCCGCTcctgct                            >  2:355303/1‑143 (MQ=255)                                                                                                       ggTGGGGCGGTCGCTCAAATCACGCCACGGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTgg                          <  2:462825/143‑1 (MQ=255)                                                                                                            gaCGGTCGCTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTCCCCTGACACCGGGTGGCTTCTTCAGTTTCGTCAACGTCGCTAATAACTTCGCCAACAGCGATGATGCTCGCATCt                     >  1:205786/1‑143 (MQ=255)                                                                                                                gTCGCTCCAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc                 <  1:784092/143‑1 (MQ=255)                                                                                                                    cgcAAATCACGCCACAGCGCGTGGGGCAGGCATGTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa             <  1:185471/141‑1 (MQ=255)                                                                                                                    cTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa             <  1:95839/143‑1 (MQ=255)                                                                                                                    cTCAAATCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa             >  1:561162/1‑143 (MQ=255)                                                                                                                         aTCACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGAt        >  1:166140/1‑143 (MQ=255)                                                                                                                           cgcGCCACGGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa      <  2:315704/141‑1 (MQ=255)                                                                                                                           cACGCCACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATaa      <  2:768308/143‑1 (MQ=255)                                                                                                                               ccACAGCGCGTGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac  >  1:555697/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                 TGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCAC  >  NC_000913/2661676‑2661944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑