| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,560,455:1 | +G | 100% | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 95.0% | 125.1 / ‑1.5 | 40 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (16/22); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT > NC_000913/3560313‑3560591 | atCGATAAACGGCGCCGAGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑Gc < 1:341689/143‑1 (MQ=255) cGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑Gcgc > 2:584060/1‑141 (MQ=255) aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑Gcgcag > 1:162703/1‑139 (MQ=255) aaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGAt < 1:196705/143‑1 (MQ=255) aaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTAGGTCTGGGTGGGCTTGGGATCGTGCCACGGCGTGTTAACGGAACTGGAAGGGAGCGGCACAGCG‑CGATGATA‑Gcgcgtgat > 2:57648/1‑136 (MQ=255) aCAGCGTCGAGCCAGGGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATc > 2:383297/1‑143 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCGTGATG‑GCGCAGGATCAGGtgt > 1:988914/1‑141 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGttt < 1:1023110/143‑1 (MQ=255) cATTGGGGAGTTGCTCCGCCACTCTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTcc < 1:127398/143‑1 (MQ=255) ttGGGGCTGTTCTGCGCCGCTGGGTGGGCGGCGCGCTCTGTGTATTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGc < 2:592107/143‑1 (MQ=255) ttGGGGATTTGCTCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGc < 1:221082/143‑1 (MQ=255) gtGGGGGTTTGCTCCGCCACTTTGCGGGCGAGGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAAATGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGc < 1:379858/142‑1 (MQ=255) gtGGGGGTTTGCGGCGCCGCTGTGCGGGCGGGGCGCTCTTTTGGTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGTTCCGc < 2:634470/142‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCt < 1:695704/143‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCt < 1:738768/143‑1 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCG‑CCCAGTTGTTG‑GAGGATCAGGGTTTGCTCCGCCAGCTCATTGAg > 1:441852/1‑143 (MQ=255) tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGAGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc < 2:600419/143‑1 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTTGGTCTGGGTGGCCGTGCGATCGTGCTACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa < 1:707976/143‑1 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa < 1:217619/143‑1 (MQ=255) gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAACTCATTGAGGTCGCGGCGAATAGTATGCgag > 2:818350/1‑141 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt < 1:777665/143‑1 (MQ=255) tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGTAGTCTGCGGGCTGa > 1:658392/1‑143 (MQ=255) gtcGGTCTTGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGACCCGGTTT‑CG‑CAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga < 2:641659/142‑1 (MQ=255) gTCTGGGTGGCCCTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAACGCCTGCTGGCTGACGGAGAAATg < 2:647826/143‑1 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAACGc > 2:286160/1‑143 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCAGGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCGTAGTCTGCGGGCTGACGGAGAGATGc > 1:252803/1‑143 (MQ=255) gcTGGGTGGCCTGGCGGTCGTGCCGCGGCGTGTGAACCGAACTGGAAGGCAGGGGCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc < 2:110985/142‑1 (MQ=255) ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCCGACGGAGAAACGCACTa > 2:166070/1‑143 (MQ=255) tGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGGCTGCCGGCTGCCGGAGAAATGCTCTAcc > 2:737525/1‑143 (MQ=255) gcTTGGGGTCGTGCGGCGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc < 2:686272/142‑1 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGct < 1:613668/143‑1 (MQ=255) cgcgcGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc < 1:18209/139‑1 (MQ=255) tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCACCACTTCggc > 2:654022/1‑142 (MQ=255) cGTGTCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 2:810925/143‑1 (MQ=255) ccACGGCGTGTTGACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTGCTGa > 2:403349/1‑143 (MQ=255) gtgtGAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc < 1:383297/143‑1 (MQ=255) ttAACCGAACTGAAAGGCAGCCCCGCACCG‑CCATGATG‑GCGCAGGATCAGGCTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg > 2:931335/1‑143 (MQ=255) aCCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg > 2:347793/1‑143 (MQ=255) tGGAAGGCAGCGCCGCACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcg > 1:164987/1‑142 (MQ=255) cgccgcCCCG‑CCATGGTG‑GCGCACGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa < 1:770561/143‑1 (MQ=255) cgccgcACCG‑CCATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTCCGataa > 1:702903/1‑143 (MQ=255) cgccccACCG‑CCATGATG‑GCGCCGGATTAGGTTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATAGTCTGCGGGCTGACGTAGAAATGCTCCACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa < 1:692707/143‑1 (MQ=255) cATGATG‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa > 1:921174/1‑143 (MQ=255) gatg‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTGTAACCAGTTCGATAATACGGTTGTGACGt > 2:1004501/1‑143 (MQ=255) | ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCG‑CCATGATG‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT > NC_000913/3560313‑3560591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |