| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 603,393 | T→A | 8.6% | intergenic (+58/+23) | pheP → / ← ybdG | phenylalanine:H(+) symporter PheP/miniconductance mechanosensitive channel YbdG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 603,393 | 0 | T | A | 8.6% | 156.1 / 3.9 | 57 | intergenic (+58/+23) | pheP/ybdG | phenylalanine:H(+) symporter PheP/miniconductance mechanosensitive channel YbdG |
| Reads supporting (aligned to +/- strand): ref base T (28/23); new base A (2/3); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCT > NC_000913/603251‑603535 | ggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAACCCGCTCTCATCCAt > 1:172929/1‑143 (MQ=255) atgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACCAAACGCTGTCAACCATCCGAAACAGACCTGATCCATTc > 2:914974/1‑143 (MQ=255) gatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCCTCCGAAACCGCTCTCATCCAATCGa > 1:376890/1‑143 (MQ=255) gatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGa > 1:327014/1‑143 (MQ=255) atGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga < 1:140024/143‑1 (MQ=255) cgcTTGTCGGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTTTCATCGAATGGATGagag < 1:57550/143‑1 (MQ=255) gcTTGGCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGTCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGc < 2:532292/143‑1 (MQ=255) tatctaTCTTGCTGCCGGTGTGGATTGTATTCCGGTTGACGGCAGTCAAACCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAGCGATGTCAACCATCCGAAACCGCTCTCATCGAATGGCTGAGAGCGGtttc < 1:837854/138‑2 (MQ=255) cAGCCATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttt < 1:648711/143‑1 (MQ=255) gacccTGCTGCGGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTa < 2:350398/140‑1 (MQ=255) cccggctgcCGGTGCGGATTGTATTCCTGTTTACGGCATTTACAACGCCGCGTCGGAAATAAGGCATGCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAAt < 2:311637/139‑1 (MQ=255) ctgctgCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATCCGATGAGAGCGGTTTTTTTACTTa > 2:372103/1‑143 (MQ=255) ggggATTGTATTCCTGTCTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATg < 1:54694/141‑1 (MQ=255) gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAATGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAGTTACTGCTTAAATGCAc > 1:367190/1‑143 (MQ=255) gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTACATGCAc > 1:804736/1‑143 (MQ=255) tCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTCCTGCTTAAATGCACCCGCCaga > 1:546082/1‑143 (MQ=255) gCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCACCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATc > 1:535344/1‑143 (MQ=255) aaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCCTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGA‑Cgg > 2:814389/1‑143 (MQ=255) gagagAAAGAAGGCATTGAGGGTATGTACGACAAGGCGTTGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAGGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGAc < 1:1051799/139‑1 (MQ=255) tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGCGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGCCGACt > 2:1018017/1‑143 (MQ=255) gcgaaTGAGGCATTCACGCTACATCCGACAATACGATGTCAACGCTCCGAAACCGCTCTCGTCCGCGCGGGGCGAGGAAAATAATTGATCACTGCTTAAATGCACCCGCTGGAGAGCGGATAGCAGGGC‑CGGTTGGCGACtga < 2:224592/140‑1 (MQ=255) gacttacggCATTGACGCTCCACTGCACAAATCGCTGTCACCCCTCCGAATCCGCTCTCATCCATTCGATTCTAGCGGTTTTTTTAATTACTGCTTACATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACtgat < 2:104615/136‑1 (MQ=255) aaTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGAACCCGCCAGAGAGCGAATATCATTCC‑CGGTTTGCGACtgacga > 2:1032226/1‑143 (MQ=255) aTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGaa < 1:897119/143‑1 (MQ=255) aGGCATTCACGCTACATCCGACAAAACGATGTGAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGCTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATGATTGC‑CGGTTGGCGACTGATGAAggc > 1:679704/1‑141 (MQ=255) tgccttCTCGCTACTTACGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTGAAATGCACCCGCCAGAGCGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCg < 2:363331/139‑1 (MQ=255) ggCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGc > 2:251351/1‑121 (MQ=255) ggCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGc < 1:251351/121‑1 (MQ=255) cATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCa < 1:995762/143‑1 (MQ=255) aTTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGccg > 1:787660/1‑141 (MQ=255) aCGCTACATCCGACAAAACGCTGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCa > 1:609617/1‑143 (MQ=255) cTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAAc < 1:654656/143‑1 (MQ=255) tCCTACAAAACGATGTCAACCATCCGAACCTGCTCTCATCCATTCGATGAGAGCGGTTTTTTTATTTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc < 2:102007/143‑1 (MQ=255) tCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc < 1:432041/143‑1 (MQ=255) tCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATCAAGCCGCAGACCCAActcctc > 2:962269/1‑143 (MQ=255) tCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCGTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc < 2:172929/143‑1 (MQ=255) ccGACACAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTCTTTTAATTACTCCTCAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCg < 1:682924/143‑1 (MQ=255) aaaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAAt > 1:178398/1‑143 (MQ=255) aaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATTATTGC‑CGGTTGGCGCCTAAACATTACTCAGACCAAACTCTTCGACAATc > 1:393288/1‑143 (MQ=255) aCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGGTTTTATAATTACTGCTTAAATGCACACGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGc > 1:126100/1‑143 (MQ=255) tGTCAACCATCCAAAACCGCTATGATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGACAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAat > 2:131080/1‑143 (MQ=255) aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGCCCGCGCATGTC‑TGGCGCGGTTGGCGACTGATGAAGTCGCAGACCCACCTCTTCGACAATCGCAACTAtgt > 1:242923/1‑143 (MQ=255) aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtgt < 2:656770/143‑1 (MQ=255) ccATCCGAAACCGCTCTCATCCCTTCGCTGCGACCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGa < 1:823094/143‑1 (MQ=255) aTCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CCGATGGCATCGGATTAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATc > 1:1054051/1‑143 (MQ=255) cgacgcggCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGaa < 2:1020262/136‑1 (MQ=255) gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGACGTCGCTGACCAAACTCTTCGACAATCGCAAAGTTGTTATCGatt > 1:196695/1‑141 (MQ=255) gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAat > 1:428327/1‑143 (MQ=255) aCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACGGCTTAAATGCACCCGCCAGAGGGAGAATAACAAAAC‑CGGTTGGCGACTGATTAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatata < 1:245947/143‑1 (MQ=255) aCCGCTCTCATCCATTCGATGAGAGCGGTATGTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAatata < 1:670997/143‑1 (MQ=255) cGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATc > 1:790579/1‑143 (MQ=255) ctctCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTAC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCATCGACAATAGCAAATATGTGATCGAATATATCac > 2:993071/1‑142 (MQ=255) ctctCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGCCAATCGCAACTATGTGATCGAATATAGCAg > 1:995610/1‑143 (MQ=255) aTCCATTCGATGAGAGCGGTTTTTTTAGTTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa < 2:367190/143‑1 (MQ=255) aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa > 1:159483/1‑143 (MQ=255) caaTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAAt < 1:56639/141‑1 (MQ=255) cATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGGCAATCGCAAATATGTCATCGAATATATCAGCCTAAAtt > 2:545387/1‑142 (MQ=255) aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCACAGAGCGAATATCATTTC‑CGGTTGGCGACTGAAGAAATAGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGc > 1:364146/1‑143 (MQ=255) ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCt > 1:341053/1‑143 (MQ=255) | GGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGC‑CGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCT > NC_000913/603251‑603535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |