Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 685,056 T→C 8.9% D67G (GAC→GGC)  gltL ← glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913685,0560TC 8.9% 120.4 / 2.0 55D67G (GAC→GGC) gltLglutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (20/29);  new base C (3/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCACTTCGGTTGAG  >  NC_000913/684915‑685196
                                                                                                                                             |                                                                                                                                            
cGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGACGCTTTGCCAGATCGGTTTTCTTGTc                                                                                                                                             >  2:321115/1‑143 (MQ=255)
  aGCCGGCGCTGTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTAGCATCACCTACTGATTTGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGt                                                                                                                                           <  1:729302/143‑1 (MQ=255)
    ccGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTa                                                                                                                                         >  1:214569/1‑143 (MQ=255)
      cacgcTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCCACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAAc                                                                                                                                       <  1:296436/141‑1 (MQ=255)
       gcgcTTTTTCGCGTTTTAGCCCTTTCACCTGCGCCAGGGTAAGGTTTTCGATTATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAAcc                                                                                                                                      <  2:888290/143‑1 (MQ=255)
         gcTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGCAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATAGGGTTTCTTGCCGCTAACCAc                                                                                                                                    >  2:422379/1‑143 (MQ=255)
                             tttCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCATTGCCAGATCGGTTCTCTTGTCGTTAACCACGATACCAACCACGGTGAttt                                                                                                                >  2:997787/1‑143 (MQ=255)
                                cACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGCCGTTAACCACGATACCATCGACGGTGATTTCAc                                                                                                             >  2:170921/1‑143 (MQ=255)
                                cACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGCCGTTAACCACGATACCATCGACGGTGATTTCAc                                                                                                             >  2:168547/1‑143 (MQ=255)
                                 aCCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGCTAACCACGATACCATCGACGGTGATTTCAcc                                                                                                            >  2:958672/1‑143 (MQ=255)
                                   cTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTTCCAGATCGGTTTTCTTGTCGTTAACCACGCTCCCATCGACGGTGATTTCACCtt                                                                                                          >  1:188098/1‑143 (MQ=255)
                                    tGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCCCGATACCATCGACGGTGATTTCACCTtg                                                                                                         >  1:607275/1‑143 (MQ=255)
                                          aGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCAc                                                                                                   >  1:513621/1‑143 (MQ=255)
                                              tCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAACACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGt                                                                                               <  1:282111/143‑1 (MQ=255)
                                                aGGTTTTCGATAATCGACCGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTc                                                                                             <  1:130963/143‑1 (MQ=255)
                                                   ttttCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAg                                                                                          >  1:1040644/1‑143 (MQ=255)
                                                   ttttCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAg                                                                                          <  2:811187/143‑1 (MQ=255)
                                                   ttttCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGGTAGCCACGATGCCATCGACGGTGATTTCATCTTGGTGCACGGGTTCGAg                                                                                          >  2:1005206/1‑143 (MQ=255)
                                                      tCGATGATCCACTGAGGAGGGAACGGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGTTCGGTTTTCTTCTCGTCAACCACGATACCATCGACGGTGTTTTCACCTTGCTGCACCGGTTCGAGGcc                                                                                       <  2:705028/143‑1 (MQ=255)
                                                        gATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATAGCATCCCGACGCCGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCCGCACCGGCCCGAgggcca                                                                                     >  2:988927/1‑139 (MQ=255)
                                                        gATAATCGACAGATGAGGGAACAGATCGACATGCTGGAATACCATCCCGCAGCGGGAACTCAACCAAGACAGGGTTGATATCTGCACGTTAACCACAATACGATCGACGGGGGTTTCGCCTTGCTGCACCGGTTCGAGGCCGt                                                                                     >  2:754464/1‑143 (MQ=255)
                                                           acgcGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTCGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                                                                                  <  2:984113/140‑1 (MQ=255)
                                                                aCAGCTGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGCCGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGtt                                                                             <  2:768485/143‑1 (MQ=255)
                                                                aCAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGtt                                                                             >  1:664016/1‑143 (MQ=255)
                                                                 cAGAGGAGGTAGCATATCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTGTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGttt                                                                            <  2:937797/143‑1 (MQ=255)
                                                                     tGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGCCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAAt                                                                        <  1:170921/143‑1 (MQ=255)
                                                                     tGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGCCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAAt                                                                        <  1:168547/143‑1 (MQ=255)
                                                                      gAGGGAACATCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCCACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATc                                                                       >  2:708123/1‑143 (MQ=255)
                                                                        gggAACAGCTCGTAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGGTCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAg                                                                     <  1:620909/143‑1 (MQ=255)
                                                                           accggCTCGAAATGCTGGAATGCCGTCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGt                                                                  <  2:817703/139‑1 (MQ=255)
                                                                              aGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGa                                                               <  1:339195/143‑1 (MQ=255)
                                                                                 tCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGAttt                                                            >  2:958797/1‑143 (MQ=255)
                                                                                     aaTGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCg                                                        <  1:981502/143‑1 (MQ=255)
                                                                                        gCTGGAATACCATCCTGACGCGGGAACGCAGCTTTGCCAGAGCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGaa                                                     <  1:420464/143‑1 (MQ=255)
                                                                                          tGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAAcc                                                   >  1:606416/1‑143 (MQ=255)
                                                                                           ggAATACCATCCCGACGCGGGAACGCAGCTTTGCCCGATCAGTTGTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCa                                                  <  1:194043/143‑1 (MQ=255)
                                                                                            gAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAATCACGTGATTAGCTACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGCACCAg                                                 >  1:542780/1‑143 (MQ=255)
                                                                                             aagaCCATCCCGACGCGGGAACTCAGCTTTGCCGGATCGGTTTTCTTGTCGTTAACCGCGATACCACCCACGGTGATTTCGCCTTGCTGCTCCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGa                                                <  2:388524/140‑1 (MQ=255)
                                                                                             aaTACCATCCCGCCGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTCACCACGATACCAGCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTCGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGa                                                <  2:19131/143‑1 (MQ=255)
                                                                                             aaTACCACCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGTCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGa                                                <  1:565953/143‑1 (MQ=255)
                                                                                                aCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACgg                                             <  1:959878/143‑1 (MQ=255)
                                                                                                  cATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGCTTTGCCGCAGCCAGCCgcgc                                           >  1:4443/1‑143 (MQ=255)
                                                                                                  cATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGTAACCAGACGAGc                                           >  2:654477/1‑143 (MQ=255)
                                                                                                   agcccGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCCTCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGcc                                          <  1:930230/141‑1 (MQ=255)
                                                                                                   aTCCCGCCGCGGGAGCGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGcc                                          <  2:190900/143‑1 (MQ=255)
                                                                                                         gcgctGGAGCGCAGCTTTTGCAGATCGGTTTTCTTGTCGTTAACCACTATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAac                                    <  2:275055/142‑1 (MQ=255)
                                                                                                              ggAACGCAGCTTTGCCAGATCGGTTTTCGTGTCGTTAACCCCGACACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAaccacca                               <  1:661907/143‑1 (MQ=255)
                                                                                                                     aggtgtGCCAGATCGGGTTTCGTGTCGTGAACAACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTGGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTc                        <  1:990235/138‑1 (MQ=255)
                                                                                                                       cTTCGCCAGACCGGTTTTCTTGTCGTTAACCACGACACCAGCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGc                      <  1:630049/143‑1 (MQ=255)
                                                                                                                            ccAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCtttt                 <  2:542780/143‑1 (MQ=255)
                                                                                                                              aGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCtttttt               >  1:444303/1‑143 (MQ=255)
                                                                                                                                aTCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCa             >  1:599039/1‑143 (MQ=255)
                                                                                                                                      tttCTTGTCGTTACCCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCACTTCgg       <  1:170717/143‑1 (MQ=255)
                                                                                                                                       ttCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCACTTCGGt      >  2:950194/1‑143 (MQ=255)
                                                                                                                                           tGTCGTTAACCACTGTACCATCGACGGTGATTTCACCGTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCACTTCGGTTGAg  <  2:662970/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
CGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGATTTGCCGGAACCAGACGGGCCGCAAACCACCACCACTTCGCCTTTTTTCACTTCGGTTGAG  >  NC_000913/684915‑685196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑