| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,224,242 | A→T | 7.2% | intergenic (+335/+37) | ycgI → / ← minE | protein YcgI/Z‑ring positioning protein MinE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,224,242 | 0 | A | T | 7.2% | 155.0 / 5.0 | 59 | intergenic (+335/+37) | ycgI/minE | protein YcgI/Z‑ring positioning protein MinE |
| Reads supporting (aligned to +/- strand): ref base A (25/28); new base T (2/2); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-02 | |||||||||||
GCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTT > NC_000913/1224100‑1224382 | gCATAAACATGTTGTGTTGGGCGTGGGAACAGCGTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa < 2:339745/143‑1 (MQ=255) tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc > 2:833840/1‑143 (MQ=255) aaCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACGCTg > 2:683331/1‑143 (MQ=255) agtttgtttggtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt < 1:62246/141‑1 (MQ=255) tttgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg > 1:674553/1‑143 (MQ=255) ttgttttctGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAGGACACGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGa < 1:1011408/143‑1 (MQ=255) tgttttgtGCGTGGTAACAGCCTTAAGGTGTAAGGGGGGAGGTGGAAATAGCAATGAGTAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGAGGAGGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa < 2:581441/143‑1 (MQ=255) gttttgtGGGTTGGAAAAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt < 1:567927/143‑3 (MQ=255) gCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg > 2:738809/1‑143 (MQ=255) cGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAACCCCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgc > 1:193308/1‑143 (MQ=255) gTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAACTgcg > 1:580653/1‑143 (MQ=255) tGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcgc < 1:973392/143‑1 (MQ=255) aCAGGCTTAAGGTGTAGAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa < 2:209551/143‑1 (MQ=255) ccTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGc > 2:468189/1‑143 (MQ=255) acatAAGGTGTAGAGGGGGAGGTGGCTATAGGAATGATGAGGATCAGCAAGAATACTCGCCGCTGGACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGc < 2:966049/140‑1 (MQ=255) cgtcaGGTGTAACGGGGGAGTTGGAAATAGCAATGCGGAGTATCAGCAAGAATACTGGCCGCTTTACCACAACGTGTATGGGAGGGATGAAAATCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg < 2:104918/139‑1 (MQ=255) taaggggtaaggggGGAGCTGGAAATAGCAATGGGGGGTATCAGCAAGAATACTCGCCGCTTTACCACAACGCGGATGAGAGGGATGAAAAACGCAAGGCAGAGATAGCTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg < 2:548841/143‑1 (MQ=255) tAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAttt > 1:249951/1‑143 (MQ=255) aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc < 1:592381/143‑1 (MQ=255) aaGGGGGAGGTGGAAATAGCAATGAGGAGTACCAGCAAGAATACTCGCCGCTTTACCACAACGTGGGTGACAGACATCAAACACTCAATTCAGATATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCa < 1:778910/143‑1 (MQ=255) ggggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGct > 2:111609/1‑143 (MQ=255) gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt < 1:692620/143‑1 (MQ=255) gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTGTTACAGCGGGCTTATATCAGCtcttcta > 2:780430/1‑142 (MQ=255) tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGc < 2:385356/143‑1 (MQ=255) aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAGACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc < 2:918027/143‑1 (MQ=255) aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg < 1:1010265/143‑1 (MQ=255) cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAGCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa < 2:90288/143‑1 (MQ=255) cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa < 1:841894/143‑1 (MQ=255) cAATGAGGAGTATCAGCAAGAATACGCCCCGCTATACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACATCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa < 1:277902/143‑1 (MQ=255) aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTacg > 1:384327/1‑141 (MQ=255) aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCCTCTGCTTCCGGTAAg > 2:412462/1‑143 (MQ=255) tATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc < 2:262276/143‑1 (MQ=255) gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc > 2:994069/1‑143 (MQ=255) taGAATACTCTCCGCTTCACCAGAACGTGGATGAGAGTGATGAAAAACTCATATCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGATTATTTCAGCTCTTCTGCTTCCGGTAAGGTCATGTTCAGCTTaa < 2:945070/142‑1 (MQ=255) aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCaa < 1:321416/143‑1 (MQ=255) tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa > 2:568833/1‑143 (MQ=255) ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAata > 1:52547/1‑143 (MQ=255) cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCg > 1:770879/1‑143 (MQ=255) tttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc < 1:620291/143‑1 (MQ=255) ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATc > 1:98992/1‑143 (MQ=255) gaataCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCGAGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCt < 1:73203/139‑1 (MQ=255) cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCt > 2:450178/1‑143 (MQ=255) cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCt > 1:68943/1‑143 (MQ=255) acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCtt > 1:752735/1‑143 (MQ=255) gcGTGGATGAGAGGGATGAAAAGCTCAAGGCGGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCttttt < 2:674553/142‑1 (MQ=255) ggtgggtggGGGGGATGAAAAACTCAAGGCAGAGGTAACTCTGCCTTGAAGATACATGCGCCGTTACAGCGCGCTTATTTCCGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTg < 2:267760/142‑1 (MQ=255) gTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCCTATTTGAGCTCTTCTGCTTACGGTGTGGTGACGTTCAGCTCAAGAAGAGAAATATAGCCATATTATTga > 1:663363/1‑142 (MQ=255) tGAGAGTGATGAAAGACTCAAGGTAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAg < 2:389793/143‑1 (MQ=255) gagaGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGc < 1:738809/143‑1 (MQ=255) tGAAAAACTCAAGGCAGAGATAGCTCTGCGTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCGCGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGt < 2:894607/143‑1 (MQ=255) aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAcc < 2:145936/143‑1 (MQ=255) tCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTCGCTCAAGCTGTACGGTTACCAtct > 2:592812/1‑143 (MQ=255) gcgaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCGTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct < 1:864738/140‑1 (MQ=255) cAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtctc > 1:608226/1‑143 (MQ=255) ggCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGGTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCGCTACGGTTACCATCCCAgg > 1:309711/1‑143 (MQ=255) ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc > 2:421282/1‑119 (MQ=255) ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc < 1:421282/119‑1 (MQ=255) aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAtt > 1:416169/1‑143 (MQ=255) aTAACTCTGACTTGAAGATAAATGCGCTTTTACAGCGGACTTATTTCAGGTATTCTGCTTCAGGTAAGGGCACGTTCAGCTCAAGAATAGAAATATCGCCATGTTTTTGGCCAAGCTGTGAGGGTCCCACCACAAGATCAAtt > 2:1057020/1‑143 (MQ=255) tAACTCTTCCTTGAAGCTAAATGCGCTTTTACAGCGGGCTTATTTCCGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATCGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAttt < 1:780430/143‑1 (MQ=255) | GCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTT > NC_000913/1224100‑1224382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |