| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | T→A | 100% | I690N (ATC→AAC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | A | 100.0% | 191.2 / ‑6.7 | 63 | I690N (ATC→AAC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (30/31); minor base G (0/1); total (30/33) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124946‑125220 | ggTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAacac > 2:561091/1‑143 (MQ=255) tCACCGCCACATTCTGTCGCTAACTATCCCGAACTTTATCTCTTACTACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTa < 2:514821/143‑1 (MQ=255) acaGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACg < 2:1057340/143‑1 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGaa < 2:948375/143‑1 (MQ=255) tCAGTCGCTGACTATCCCGAACTGTCTCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCCTCATGCATGACGGTCTGGAGCGTCTGTACGGTGAAAAACAAGAGAACGTTCACTACTACAACACTAACCTCAACGaaa > 2:271970/1‑143 (MQ=255) tCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTTCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGCGAACAACACGAGCACCTTCACTACTACAACACTCCGCCGAACGacc > 2:1024519/1‑141 (MQ=255) tCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGaaa < 1:216300/143‑1 (MQ=255) cAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGaaaa > 2:254348/1‑143 (MQ=255) aGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAAc > 1:971839/1‑143 (MQ=255) cGCTGACTATCCCGAACTGTATCTCTTACGCCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTAc < 2:653537/143‑1 (MQ=255) gCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTAcc < 1:1038097/143‑1 (MQ=255) cTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACca < 1:29556/143‑1 (MQ=255) cTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACCGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACca > 1:1014746/1‑143 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGCAGTTGCTGTCATCATGCATGACGGACGGGAGCGTATGTACGGTGAAACACAAGAGAACGTTTACTACTACAACACGACGCGGAACCAAAACTACAACATGc > 2:323768/1‑143 (MQ=255) ccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTCACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGc > 1:1015037/1‑143 (MQ=255) gAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCaa < 1:498275/143‑1 (MQ=255) tGTAACGCTCACGGCTCGGCGTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGACAACGTTTACTACTACAACCCTACGCTGAACGAAAACTACCACATGCCGGCAATGcc < 2:734194/143‑1 (MQ=255) tATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCgg > 1:205058/1‑143 (MQ=255) ctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGGTTCCTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAAAGCCGGCAgga > 2:548510/1‑142 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTg > 1:699442/1‑143 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTg > 1:907104/1‑143 (MQ=255) tACGACTCGGCGTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACCTTCCGGCATTGCCggcatgtgg < 2:804549/143‑8 (MQ=255) tACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGc < 1:563895/143‑1 (MQ=255) ccGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGa > 1:807061/1‑143 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGACAAACAAGAGAACGTTTGCTACTACAACACTACGCTGAACGAAAACTACCACATGCAGGCAATGCCGGAACGAGCTGAAGAAgggc > 2:362520/1‑141 (MQ=255) gcttgcgATGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCg < 2:463804/143‑1 (MQ=255) cgACGTTGTTGTCATCATGCATGACGGTCTGTAGCGTATGTACGGAGAAAAACAAGAGAACGTTTACTACTACCACACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 2:783154/143‑1 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCAGGTGCTGAGGACGGTATCCGTAAAgg > 1:342417/1‑143 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGCGGAAGCTATCCGTACAgg > 1:546547/1‑143 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAgg > 1:672306/1‑143 (MQ=255) ttGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATc > 1:623258/1‑143 (MQ=255) tcatcaTGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaaa < 2:205058/143‑1 (MQ=255) tcagcaTGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTGGGTCGGAAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaaa < 1:775340/143‑1 (MQ=255) catcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAACAGACAGTCCCTGGTTGTAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAAc < 1:1054625/143‑1 (MQ=255) gcgtgAGGGTCTTGTGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTATAGGTATCTACAAACTCGaaa < 2:132372/140‑1 (MQ=255) gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 2:623736/143‑1 (MQ=255) gACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGACGGTGCTGAGGAAGGTATCCGTAACGGTATCTCCGAACTCGCACCTAt > 1:547510/1‑143 (MQ=255) agggTCTGTAGCGTACTTACGGTGAAAAACAAGAGAACGTTGACTACTACAACACTACGCTGAACGAACACCACCACATGCCGGCAATGCCGGAAGGTGCTGATGAAGGTATCCGTAAAGGTATCTACAAACTCGAACCTAtt < 1:622532/141‑1 (MQ=255) aCGGTCTGGAGCGTATGTGCGGCGAAAACCAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt < 2:835000/143‑1 (MQ=255) gTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTCTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:484710/143‑1 (MQ=255) ccggAGCGTATGTACGGTGAAAAACAAGAGACCGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAgg < 1:682750/141‑1 (MQ=255) gTATGGACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa < 1:898933/143‑1 (MQ=255) gTATGCACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa < 1:226882/143‑1 (MQ=255) tATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTAAAGGTAGCAAAg > 2:498567/1‑143 (MQ=255) aCGGTGAAAACCAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 1:939986/143‑1 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 2:407103/143‑1 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACCAACGCTAAACTATTGAAGGTAGCAAAGGTAAAGt > 2:464903/1‑143 (MQ=255) gTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGtt > 1:966593/1‑143 (MQ=255) gTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGtt > 1:921928/1‑143 (MQ=255) tcaaaacCAAGAGAACGTTTACTACAACAACACTCGGCTCAACCAAACCTACCTCCTGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCTTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTc < 2:508388/141‑1 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTc > 2:917280/1‑143 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGAAGCAAAGGTAAAGTTc > 2:869583/1‑143 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTcgg > 1:281811/1‑141 (MQ=255) aaaaaCAAGAGAACGGTTACTACTACAACACTACGCTGAACGAAAACTACCTAATACTATCAATTCCTGAAGGTGGTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAg < 1:489290/143‑1 (MQ=255) aaCAAGAGAACGTTTACTACTCCCGCACTGCGCTGAACGAAAACTACCACATGCCGGCAAGGCCGGGAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGACTCTATTGAAGGTAGCAAAGGTAAAGTTCAgctg < 1:269779/143‑1 (MQ=255) aaaagcaCGTTTACTATTGCATCAGCACACTGAACGTAAACTACCACATGCCTGCAATGCCGGAAAGTTCTGAGGAATGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTc < 2:103463/137‑1 (MQ=255) aaGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAACCGTAAAGGTATCTACAAACTCGAGACGATGGAAGACGGCAAAGGCAAAGTTAAgctagtc > 2:570790/1‑139 (MQ=255) gagaACGCTGACTATGAGAACACTACGGTGGACGAAAACTACCACATGCCGGCAATGCCGGAAAGTGCTGAGGAAGGTAGCCGTAAAGGTATATACAAACTCGAAACTATTGAATGTAGCAAAGGTAAAGTTCAGCTGCTCgg < 2:875715/143‑1 (MQ=255) agcGTTTCCTACTAGAACGCTACGCTGAACGAGATCGACCACATGCCGGCAATGGCGGAAGGTGCTGAGGAAGGTAGCCGGAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTc < 2:997569/141‑1 (MQ=255) aaCGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTc > 1:519529/1‑143 (MQ=255) ctactacAAGGCTACGTTGAAGGAGAAGTACCAGATGGCGGGAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGACATGTAGGAGGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTa < 2:867858/143‑1 (MQ=255) ctactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTa > 2:489414/1‑143 (MQ=255) tactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGCGGCTTGCTGAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAt > 1:537862/1‑143 (MQ=255) tactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCGAAGGTAAAGTTCAGCAGCTCGGCTCCGGTTCTAt > 1:112866/1‑143 (MQ=255) | GGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124946‑125220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |