| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,666,468 | A→T | 6.5% | Q385L (CAG→CTG) | clcB → | putative chloride:H(+) antiporter ClcB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,666,468 | 0 | A | T | 6.5% | 163.3 / 2.5 | 60 | Q385L (CAG→CTG) | clcB | putative chloride:H(+) antiporter ClcB |
| Reads supporting (aligned to +/- strand): ref base A (23/32); new base T (2/2); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01 | |||||||||||
CGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTT > NC_000913/1666326‑1666600 | cggaGCCTGGCATTATGGTTCCCTGATGGCGACGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCa < 2:738618/140‑1 (MQ=255) gCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTa < 1:517128/143‑1 (MQ=255) tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGGTGATGCTATGTCAAGCGACCGGGGCGTCTCCGCCAccc > 2:1007370/1‑141 (MQ=255) tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACGGTTGGCGGCGACCGCGCACGCGCCGATTATGTCGACGTTGATGATATGGGAAAGGACCGGGGGGTATCAGCGACTc > 1:218375/1‑143 (MQ=255) gATTTTGGTGCCCGGGTGGCGAAGAAATTACACTTTTACTCGGATGGACCGGGATGGCGACACTGTTGGGGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTcccc < 2:1013719/143‑1 (MQ=255) aTTATGGTGCCCTGATGGCGAAGAAATTACACTTTTCCTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg < 1:683338/143‑1 (MQ=255) gtttCCCTGATGGCGAAGAATTTACACTTTTACTCGGATTGACCGGGAGGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCG‑Gtcat < 2:114220/141‑4 (MQ=255) gTTCCCTGAGGGCGAAGAACTTACACTTTTACTCGGATGGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTat < 2:237246/143‑1 (MQ=255) ttCCCTGATGGCGAAGAAATTACACTTTTACTCGGATAGACCGGAGTGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTGGATGGTATGTGAAATGACCGGGGAGTGTCTGCTGCTCCGCG‑Ggttgtt > 1:546967/1‑143 (MQ=255) tCCCTGAGGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTattg < 1:1016581/143‑1 (MQ=255) tctcaTGGCGAAGAAACTACCCTTTTACTCGTATTGACCGGGATGGCGACACTGTGGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTattgat < 1:771363/139‑1 (MQ=255) ccTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTattgat < 2:893248/143‑1 (MQ=255) gATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTCCTCCCCG‑GTTTATTGATTGc < 1:704638/143‑1 (MQ=255) gATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGc > 1:760168/1‑143 (MQ=255) gggCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTTCCt < 1:781757/142‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg > 1:735371/1‑124 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg < 2:735371/124‑1 (MQ=255) gaagaaATTACACTTTTACTCGGATTGACAGGGATGGCGACACTGTTGGCGGTGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGCGAGTATCAGCTACTCCCCG‑GCTTATTGCCTGCTTGCgc > 2:784003/1‑142 (MQ=255) ttACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTAGCGGCGACCACGCACGCGCCGCCCATGTGGACGGTGATGCACACTGAAATGACCGGGGAGTCACAGCTACACCCCC‑GGTTCTTGATTGCCCGCGTaaccgcg > 2:921655/1‑138 (MQ=255) cGGATTGGACGGGATGGCGACACTGTTGGCGCCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc < 1:648514/143‑1 (MQ=255) cGGATTGACCGGGCTGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc < 1:987288/143‑1 (MQ=255) ggctggcccGCGATGGCGACACGGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg < 1:162281/136‑1 (MQ=255) ggATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTGATTTcgcg > 1:301546/1‑143 (MQ=255) ggATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGAGGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg < 2:468113/143‑1 (MQ=255) aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCAAGCGCTGATTATGTCGACGTTGCTTATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGa < 1:663034/143‑1 (MQ=255) aCCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGAGGtt > 1:747290/1‑143 (MQ=255) ccGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTa < 1:168904/143‑1 (MQ=255) tGGCGACACAGGGGGCGGCGAACACGCACGCGCCGATTATGACGACGTTGATGATAGGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGt < 1:298484/143‑1 (MQ=255) gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAct > 1:788405/1‑143 (MQ=255) gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATCTCGCGGACGTTACACCGTGAct > 2:188225/1‑143 (MQ=255) cacTGGAGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGActct < 1:982345/143‑1 (MQ=255) acTGTTGGCGGCGACCGCGCACGCGCCGATTATGTCGTCGTTGATGATATGTGAAATGACCGGGGAGTGTCAGCTACTCCCCG‑GTTTATTCATTGCCTGCGTAATTGCGTCGGTAATTTCGCCGACGTTACACCGTGACtcta < 2:408903/143‑1 (MQ=255) ttGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCGCCG‑GTTGATTGATTGCATGGGTAATTGCGTAGGTAATTTCGGGGAAGTTACAACGTGAAtctatagg > 2:584482/1‑140 (MQ=255) tGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGTTTGCCTGCGAAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatctgc > 1:288089/1‑141 (MQ=255) cggcgACCACGCACGCGCCGATGATGTCTGCGGTGATGAGATGTGAGATGGTCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGc < 1:1021230/143‑1 (MQ=255) ggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTATAAGCGGAGATGATT‑ATTGGCAAACAAATGGCGGCGGTAATTCCGCGGCAGTTACCCCGTGACTCTCTCTACCGcc > 2:1034923/1‑143 (MQ=255) cgACCGCGCACGCGCGGATTATGTGGACGTTGCTGATATGTGAAATGACGGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAg < 2:1056781/143‑1 (MQ=255) cgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGCCATTTCGCGGACGTTCCACCGTGACTCCATCTACCGccgc > 2:996183/1‑141 (MQ=255) aCCACGCAGGCGCCGATTATGTCGACGTTGAGTGTATGTGAGCTGTCCGCTGAGTATCAGCTACTCCCCG‑GCTGATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGca < 2:916337/143‑1 (MQ=255) cacgcacgCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACAGCGTGACTCTATCTACCGCCAGcaca > 1:595128/1‑143 (MQ=255) cacgcacgCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGcaca < 1:709071/143‑1 (MQ=255) gcacgcGCCGATTATGTCGACGTTGATAATATGTGAAATAACCGGGGAGTATCAGCTACTCCCCG‑GGTTAGTGATTGCCTGCGTAATTGCGTCGGTAAGTTCGCGGACGTTACACCGTGACTCTATCTACTGCCAgaaacacg > 2:391741/1‑136 (MQ=255) ccgcgCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcg < 1:707531/142‑1 (MQ=255) cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATGGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcag < 1:377742/143‑1 (MQ=255) ccGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTGCACCGTGACTCTATCTACCGCCAGCACACTGCgcagca > 1:660919/1‑143 (MQ=255) ttATGTCGACGTTGATGATATGTTAAAGGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGc < 2:28340/143‑1 (MQ=255) gTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAGTTGCGCGGCCGTTACACCGTGACTCTATCCACCGCCAGCGCACTTGTCAGCACAGCtggc > 2:852211/1‑140 (MQ=255) gTCGACCTTGATGATACGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAg < 1:67534/143‑1 (MQ=255) tCGACGGTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGc < 1:314691/143‑1 (MQ=255) gTTGATGATCTGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGCCGTTACACCGTGACTCTATCTACCGCCAGCACACTGCCCAGAATACCCAAGCGGAta > 2:1021318/1‑142 (MQ=255) gTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCGACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATg > 1:442609/1‑143 (MQ=255) gatgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTAt > 1:613642/1‑143 (MQ=255) tgtgCAATGACCGGGGAGTATCAGCTACTCCACG‑GTTTATTGATTGCCTGCGTACTTGCGTCGGTACTTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTTCGCAGCATAGCTAAGCGGATGTATTGCCCCa < 2:862959/143‑1 (MQ=255) ctgcccGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAc < 2:779761/139‑1 (MQ=255) tGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACg < 1:777237/143‑1 (MQ=255) ccGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTg < 1:230935/143‑1 (MQ=255) cGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTACTTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCCTTGCTACGCGGATGTATTGCCCCACTTCACGCTGt > 2:242828/1‑143 (MQ=255) cGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACg > 1:41208/1‑139 (MQ=255) cGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACg < 2:41208/139‑1 (MQ=255) cGGGGAGTAGCTGATACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGt > 1:846738/1‑143 (MQ=255) ggggAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCAAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCCCGCTGtt > 1:55979/1‑143 (MQ=255) | CGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCG‑GTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTT > NC_000913/1666326‑1666600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |