| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,872,791 | A→G | 7.4% | T251A (ACT→GCT) | dgcJ → | putative diguanylate cyclase DgcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,872,791 | 0 | A | G | 7.4% | 150.7 / 2.4 | 55 | T251A (ACT→GCT) | dgcJ | putative diguanylate cyclase DgcJ |
| Reads supporting (aligned to +/- strand): ref base A (25/25); new base G (2/2); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01 | |||||||||||
CCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGG > NC_000913/1872651‑1872927 | ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACt < 1:297090/143‑1 (MQ=255) cGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATAAGTTTTATAcgc > 2:910532/1‑141 (MQ=255) ggatggTCAAGCATTTACCAGGATCGGCGTAGTAGACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTAGAAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCa < 2:51303/138‑1 (MQ=255) ttGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa < 2:956277/143‑1 (MQ=255) gAGCATTTACCAGGCTCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGcc < 1:218320/143‑1 (MQ=255) gCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcct > 1:957817/1‑143 (MQ=255) tACCAGGAGGAGCGTAAATAACACAGCGTGATGAGGTTGCTGGCGCCGGGACATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcctctcct < 2:504238/143‑1 (MQ=255) cgggATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTg > 1:618805/3‑143 (MQ=255) cAGGATCAGCGTACTAAACAGAACGTGATGAGTGTGCTGACGCCGGTATACGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTg < 1:1047153/143‑1 (MQ=255) ggATCAGCGTACTAAACAGAACGTGATGAGGTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGc < 1:909627/143‑1 (MQ=255) gATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCg > 1:13207/1‑143 (MQ=255) aGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGtttt < 1:363052/143‑1 (MQ=255) aGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGCCGtttt > 2:1022074/1‑143 (MQ=255) cGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGAAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTtct < 1:762810/143‑1 (MQ=255) cTAGACAGAACACCATGAGTTTGCTGCGGCCGGTATATGACGCAGGGCTGCTAAAAGAGATTGTGCTGCTCGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAAt < 1:17506/143‑1 (MQ=255) cTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAATAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAAt < 1:32754/143‑1 (MQ=255) tAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAAta > 2:883314/1‑142 (MQ=255) agcGTGAGGAGTTTGCTGACGCCGGTAGATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCt < 1:844686/141‑1 (MQ=255) aCGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATGTTAAC‑AAAACAATCTGCGGAATACCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCCCggcta > 1:951941/1‑139 (MQ=255) gTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACg > 1:17899/1‑138 (MQ=255) gTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACg < 2:17899/138‑1 (MQ=255) aGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatac > 1:907925/1‑143 (MQ=255) tttGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgataccg > 1:138105/1‑143 (MQ=255) gCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCAGAATATCTTTTATACTCATGACAGCGCTCGCCTCTGGCGTTTTCTCTCTGTCACGCTAACCCATACCCAGTCGGGgc > 2:589487/1‑143 (MQ=255) cGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGgcgc < 2:723076/143‑1 (MQ=255) ggTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGcgcg > 1:178297/1‑143 (MQ=255) gTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTat > 2:440128/1‑143 (MQ=255) tCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGCCATTatc > 1:881187/1‑143 (MQ=255) cGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTACCCGATACCGCTTCGGGGCGCGACCTTatcc > 1:196195/1‑142 (MQ=255) aGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTCACCGATACCGATCCGGGGCGCGACATTAtcatca > 1:523992/1‑143 (MQ=255) cAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCa < 1:842235/143‑1 (MQ=255) cAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCa < 1:561279/143‑1 (MQ=255) cTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCagag < 1:1063063/143‑1 (MQ=255) cTAAAAGGGAGTGTGCGGCTGGATATTGACAAAAGCGATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCGCGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCagag < 1:1027752/143‑1 (MQ=255) aaaGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGGTTCGGGGCGCGACATTCAAATCAACGagtgtgc > 2:1024763/1‑138 (MQ=255) aaaGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGa < 1:50383/143‑1 (MQ=255) tggtgCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCGGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGTGCGCGACATTATCATCAACCAGAGCGAAGATAAt < 2:157014/141‑1 (MQ=255) tgctgcTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTg > 1:749475/1‑143 (MQ=255) ctgctgGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCATACAGAGCGAAGATACTCTGtt > 2:255626/1‑143 (MQ=255) tgctgGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGCCCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATCATCTGTTc > 1:201388/1‑143 (MQ=255) tgctgGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGCCACGGAGGCCGGGCGCGACCGTATCATCAACCAGAGCGACGATGCTCTGGTc > 2:222936/1‑143 (MQ=255) gATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGCTACCGATTCGGGGCGCGACATTATCATGAACCAGAGCGAAGAGAGTCTCTTCCAGTa > 1:506522/1‑143 (MQ=255) atatTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTAt < 2:618805/143‑1 (MQ=255) tAACAAAAACAATCTGCGGCATATCTTTTATCCTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCa < 1:910532/143‑1 (MQ=255) tAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTGTCATCAAACAGAGAGAAGATAATCTGTTCCAGTATGTCa > 2:615148/1‑143 (MQ=255) tAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCa < 1:1024763/143‑1 (MQ=255) agcaaacCAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAACGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGt < 2:740526/140‑1 (MQ=255) aCAAAAACAATCTGCGGAATATCTTTTATGCTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatac > 1:463969/1‑82 (MQ=255) aCAAAAACAATCTGCGGAATATCTTTTATGCTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatac > 1:463970/1‑82 (MQ=255) aCAAAAACAATCTGCGGAATATCTTTTATGCTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatac < 2:463970/82‑1 (MQ=255) aCAAAAACAATCTGCGGAATATCTTTTATGCTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatac < 2:463969/82‑1 (MQ=255) gaaatCAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCCATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTCTCATCAACCAGAGCGAAGATACTCTGTTCCACTATGTCACttc > 2:874092/2‑142 (MQ=255) aaaaCAATCTGCGGAATATCTTTTATACTCATGCCCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTAc < 2:294297/143‑1 (MQ=255) aTCTGCGGAATATCTTTTACACTTAGGTCCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCAt < 2:256307/143‑1 (MQ=255) ttttATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATACTCTGTTCCAGTATGTCAGTTACGTCCATGACTTCCCGtgccg > 1:434498/1‑141 (MQ=255) | CCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGG > NC_000913/1872651‑1872927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |