Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 10.8% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT10.8% 77.0 / 2.4 37I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base A (13/17);  new base T (2/2);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

ATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAA  >  NC_000913/2081546‑2081827
                                                                                                                                                |                                                                                                                                           
aTCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGACCTCTTGTTCCGTTCCCAGGGCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCaaaa                                                                                                                                             <  1:868457/143‑1 (MQ=255)
          tatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCTAGAACTCTTGTTCCCTTACCAGGAAAATAAGGACTTTATCGCCGTACTAGATGCGTTCCGTTGCGACCTTTTT‑‑CCCACGACGCAAGATTTGCACCgt                                                                                                                                   >  2:127420/1‑143 (MQ=255)
           ttttGGCTTTGATATCAGTTGATGCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGCTCCCAGGCCGAGATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAATTTTGCACCgtg                                                                                                                                  <  2:4938/142‑2 (MQ=255)
           gtttGGCTTTGAGATGTGGCAGTGCGTCTTTCGGTAGCACCATCCAGAGCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCgta                                                                                                                                  <  2:791868/142‑1 (MQ=255)
            tttGGCTTGGATATGTGGAATTTTCGCTTGCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAATTTTGCACCgtgg                                                                                                                                 <  2:818894/143‑3 (MQ=255)
                gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCTAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTaaa                                                                                                                             <  2:105075/143‑1 (MQ=255)
                gCTTTGATATGTCGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTaaa                                                                                                                             <  2:105126/143‑1 (MQ=255)
                  tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGCTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTGTTT‑‑CCCTCGGCGCACAATTTGCACCGTAGTAAAGt                                                                                                                           >  2:117537/1‑143 (MQ=255)
                     gagatGTTGAATTGCGGCTTTCGGTAGCACCTTCCATAACTCTTGTTCCGGTCCCAGGGCGATATTGTCTTTATGTCCATACCAGATGCGTTCCGTTCCGACCTGTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGtttt                                                                                                                        <  2:14595/140‑1 (MQ=255)
                     gATATGTGGAATTTCGGCTGTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGtttt                                                                                                                        <  1:689182/143‑1 (MQ=255)
                          gtgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGc                                                                                                                   <  1:984503/143‑1 (MQ=255)
                           tgGAATTTCGGCTTTCTGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcc                                                                                                                  <  2:1011556/143‑1 (MQ=255)
                           tgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCCCGGTGCACAATTTGCACCGTAGTAAAGTGTTGTTGcc                                                                                                                  >  2:84582/1‑143 (MQ=255)
                            ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTAGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑ACTACGTTCCAAAATTTACATCGTAGTAAAGTTTTTTTGccc                                                                                                                 >  2:537120/1‑143 (MQ=255)
                              aaTTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGCTGCCAACCTTGCACAGCAGTCACAATTTGTTGccccc                                                                                                               >  2:716313/1‑143 (MQ=255)
                                    ggCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCa                                                                                                         <  1:827030/143‑1 (MQ=255)
                                    ggCTTTCGGTAGCACCATCCAGAACTCTGGTTCCGTCCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCa                                                                                                         <  2:359214/143‑1 (MQ=255)
                                                cACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                                                             >  2:406132/1‑143 (MQ=255)
                                                cACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACC‑TTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTTCCCCCAACGCAGTAATTTTCTTGc                                                                                            >  1:165883/1‑143 (MQ=255)
                                                 aCCATCCAGAACTCTCGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                                                                                            <  1:617891/143‑1 (MQ=255)
                                                   cATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGACCCCAT‑‑GACCGGTGTGGCCGACGGTGGTCCCCTTGCACCGTAGTAAATTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCct                                                                                          <  1:492975/143‑1 (MQ=255)
                                                          aaCTCTTGTTCCTTTCCCATGTCGATATGTTCTTTATCGCCATACCAGATGCTTTCCGTTCCGACCTTTTT‑‑CCCACTGTGCAAAATTGGCACCGTAGTAAAGTTTCGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTc                                                                                   <  2:668148/143‑1 (MQ=255)
                                                              ctTGTTCCGTTCCCATGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGa                                                                               <  2:680218/143‑1 (MQ=255)
                                                                  ttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCCGTAATTTTCTTGCCTCTTCCTCCCCACCtt                                                                           >  1:807347/1‑143 (MQ=255)
                                                                    ccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtttt                                                                         <  1:589461/143‑1 (MQ=255)
                                                                             ggTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCa                                                                <  2:919287/143‑1 (MQ=255)
                                                                                tcttttcttCTTTATCGCCATACCATATCCGTTCCGTTCCTACCTTTTT‑‑CCCACGGTGCAAAATTTGCCCCGTAGTAACTTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                             <  2:924396/136‑1 (MQ=255)
                                                                                 gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTCGCACTGTCAGccc                                                            >  2:968187/1‑143 (MQ=255)
                                                                                     ttttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt                                                        >  1:891268/1‑143 (MQ=255)
                                                                                                       cAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCCACGCAGTAATTTTCTTGCCTCTTCCTCCCGACCTTTTGCACTGTCAGCCCCCCTTCCCACTCCCATCAgacg                                      >  2:396554/1‑143 (MQ=255)
                                                                                                           tGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGc                                  >  1:476456/1‑143 (MQ=255)
                                                                                                                            ttttt‑‑CCCACGGTGCAAACTTTGCTCCTTAGGAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGCGACGACGCTGCCCATCTACAGCCGa                 >  2:131798/1‑143 (MQ=255)
                                                                                                                            ttttt‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCCGCCCCCATTACCACTGCCATGAGACGACGCTGCCCATCTACAGCCGa                 >  1:283088/1‑143 (MQ=255)
                                                                                                                            ttttt‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAAAGTCAGCCCCCATTGCCATCGTCTTTATACGACGCTGCCCCTCTACAGccca                 >  2:10482/1‑141 (MQ=255)
                                                                                                                                   cccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCaa            <  2:48645/143‑1 (MQ=255)
                                                                                                                                     cACGGTGCAAAATTTGCACGGTAGTAGAGTTTAGTTGCCCCCAACGCAGTAATTTTCTTGCTTCTTCATCACGACCTTTTGCACTGTAAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCCACAGCCGAAGCAATg          >  2:810792/1‑143 (MQ=255)
                                                                                                                                      aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCGGCCCATCTACAGCCCAAGccacgc         >  2:680510/1‑138 (MQ=255)
                                                                                                                                        ggTGCAAAATTTGCACCGTAGTACAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCTGCTCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgaga       <  1:172521/143‑1 (MQ=255)
                                                                                                                                             aaaaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATGACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTaaa  <  2:958333/143‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
ATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAA  >  NC_000913/2081546‑2081827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑