Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,214,600	G→A	6.6%	C15C (TGC→TGT)	btsS ←	high‑affinity pyruvate receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,600	0	G	A	6.6%	167.9 / 3.3	61	C15C (TGC→TGT)	btsS	high‑affinity pyruvate receptor
Reads supporting (aligned to +/- strand):  ref base G (34/23);  new base A (2/2);  total (36/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-02

GCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGA  >  NC_000913/2214458‑2214736                                                                                                                                               |                                                                                                                                         gCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACg                                                                                                                                          <  1:10326/143‑1 (MQ=255)     cccAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGcaca                                                                                                                                      >  1:971009/1‑143 (MQ=255)       cAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATc                                                                                                                                    >  1:103382/1‑143 (MQ=255)              ggagCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGaa                                                                                                                             <  1:147430/140‑1 (MQ=255)                 gCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagca                                                                                                                          >  1:700073/1‑143 (MQ=255)                  cccATGATGCAGAAGATCGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACTGTTATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagcag                                                                                                                         <  1:542171/143‑1 (MQ=255)                  ccaatgatgCAGAAGATAGAAAAGACGATGTAGCAGAGAAATTTATACGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagcag                                                                                                                         <  2:295411/140‑1 (MQ=255)                   ccATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagcagc                                                                                                                        >  1:1027782/1‑143 (MQ=255)                      tgatgCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAagcagcagc                                                                                                                     <  1:808849/143‑1 (MQ=255)                        aggCAGAAGAAGGCAAAGACGATGTAGCAGAGAAAGTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACGCATTAACCATGCAACGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCaa                                                                                                                   <  2:292951/141‑1 (MQ=255)                          gCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAaca                                                                                                                 >  1:211588/1‑143 (MQ=255)                          gCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAaca                                                                                                                 >  1:273597/1‑143 (MQ=255)                            agaagaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTACCCATGCAATGACTAAAAAAACGCACATATGCTGAAGCAGCAGCAACAcc                                                                                                               >  1:165424/1‑143 (MQ=255)                                gaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGAt                                                                                                           >  1:890862/1‑143 (MQ=255)                                   ggAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACACACATCTGCTGAAGCAGCAGCAACACCAGATTaa                                                                                                        >  1:893793/1‑143 (MQ=255)                                   ggAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACACACATCTGCTGAAGCAGCAGCAACACCAGATTaa                                                                                                        >  1:884891/1‑143 (MQ=255)                                     aaaaGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATAaaaa                                                                                                      >  2:202471/1‑143 (MQ=255)                                      aaaGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAAt                                                                                                     >  1:33052/1‑143 (MQ=255)                                      aaaGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAAt                                                                                                     >  1:251353/1‑143 (MQ=255)                                       aaGACGAGGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATc                                                                                                    <  1:142427/143‑1 (MQ=255)                                        cgACTATGTAGCATAGATATTTATGCGGCAAACTAACTATGATCTGCATTAACGTTATTAATAATGGCGTTTTACTCATTAACCATGAAATGACTAAAAAAAAGAGCTACATCTGAATCAGCAGCAACACCAGATTAAAATCg                                                                                                   <  2:326855/142‑1 (MQ=255)                                         gACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGAGTAAAATCGt                                                                                                  >  1:924022/1‑143 (MQ=255)                                          aCGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTa                                                                                                 <  1:801953/143‑1 (MQ=255)                                           cGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTAc                                                                                                >  1:83147/1‑143 (MQ=255)                                                          aTTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACcac                                                                                 >  1:350803/1‑143 (MQ=255)                                                          aTTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACcac                                                                                 >  1:548556/1‑143 (MQ=255)                                                               tGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCCGACGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTc                                                                            >  1:542061/1‑143 (MQ=255)                                                                    cAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAAGGACTAAAAAGACGCACCTCTGCTGAAGCAGCAGCAACACCAGACTAAAACCGCACATACCCGCAAACCCCCCTGccccgc                                                                       >  2:685666/1‑140 (MQ=255)                                                                         gAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTaaacc                                                                  >  2:344771/1‑141 (MQ=255)                                                                            ccGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAAcgcg                                                               >  1:82553/1‑143 (MQ=255)                                                                                   cTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCGACACCAGATTAAAATCGTACATACCCGCAAACCACACTCCCCTTTAAAACGCGTAacata                                                        >  2:250582/1‑143 (MQ=255)                                                                                    tGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAacatac                                                       >  2:593088/1‑143 (MQ=255)                                                                                        ttAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCCCAAACCACACTTCCCTTTAACACGCGTAACATACATTg                                                   >  2:45186/1‑143 (MQ=255)                                                                                               tATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCGGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGt                                            <  2:947105/143‑1 (MQ=255)                                                                                                 tGAATAATGGCGGTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGttt                                          <  1:670508/143‑1 (MQ=255)                                                                                                   aataatGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTaa                                        <  1:1057316/143‑1 (MQ=255)                                                                                                   aataatGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACACACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTaa                                        <  2:884891/143‑1 (MQ=255)                                                                                                   aataatGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACACACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTaa                                        <  2:893793/143‑1 (MQ=255)                                                                                                    atcatGGCGTCTTACTCACTAACCATGCAATGACTAAAAAAACGCACCTCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAAc                                       <  1:432703/140‑1 (MQ=255)                                                                                                    ataatGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAAc                                       <  2:340721/143‑1 (MQ=255)                                                                                                    agattGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAAc                                       <  2:25933/139‑1 (MQ=255)                                                                                                        tGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAActttc                                   <  2:103382/143‑1 (MQ=255)                                                                                                         ggCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTCACACACCTTGCCCGCGTTTACctttct                                  >  1:899010/1‑143 (MQ=255)                                                                                                             ttttACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCACACCACACTTCCCTTTAAAGCGCGTACCATACATAGCCTGCGTTTAActttctatta                              >  1:215790/1‑139 (MQ=255)                                                                                                              tttACGCATGAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAAGCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGaa                             <  2:766865/143‑1 (MQ=255)                                                                                                                tACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTATGAAct                           >  2:623344/1‑143 (MQ=255)                                                                                                                   tCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATATCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCtt                        >  1:554947/1‑143 (MQ=255)                                                                                                                    cATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTg                       <  1:1048454/143‑1 (MQ=255)                                                                                                                     aTTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACCCTTCCCTTTAAAACGCGTGACATACCTTCCCTGCGTTTAACTTTCTTTGCCCTCTTGc                      >  2:1013888/1‑143 (MQ=255)                                                                                                                     aTTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGc                      <  2:273597/143‑1 (MQ=255)                                                                                                                     aTTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCACTTTAAAACGCGTAACATACATTGCCTGAGTTTAACTTTCTATGAACTCTTGc                      >  1:224788/1‑143 (MQ=255)                                                                                                                     aTTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTCCCCTTTAAAACGCGGAACATACCTCGCCCGCGCTCAACTTTCTGTGCActctacc                      >  2:272055/1‑140 (MQ=255)                                                                                                                      atAACCACTCAATGACTAACAAAACGCACATCTGCTGAAGCAGCCGCAACACCAGATTAAAATCGTACTTTCCCGCAAACCACACCTCCCTTTAAATCGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCa                     <  1:169303/142‑1 (MQ=255)                                                                                                                       tAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCGAACCACAATTCCCTTTAAAACGCGTAACATACATTGCCTCCGTTTAACTTTCTTTGCACTCttctcg                    >  2:910933/1‑139 (MQ=255)                                                                                                                         aCCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGaa                  <  2:83147/143‑1 (MQ=255)                                                                                                                             agCAATGACGAAAAAAACGCACATCTGCTGCACCAGCAGCAACACCATATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAAt              <  2:687517/142‑1 (MQ=255)                                                                                                                                 aTGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACCTACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATgaga          >  1:163061/1‑143 (MQ=255)                                                                                                                                 aTGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTACCTTCCTCTGAACTCTTCCCGCCAAATgagt          >  1:311924/1‑142 (MQ=255)                                                                                                                                   gACCAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTATAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATGAGAAt        <  2:572312/143‑1 (MQ=255)                                                                                                                                        aaaaaaCGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTATTCCATGAAGATGAGAATTCGTg   >  2:503629/1‑143 (MQ=255)                                                                                                                                        aaaaaaCGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGCACTCTTGCAGAAAGATGATAATTCGTg   >  2:64443/1‑143 (MQ=255)                                                                                                                                         aaaaaCGCACATCTGCGGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGa  <  2:971009/143‑1 (MQ=255)                                                                                                                                               |                                                                                                                                         GCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGA  >  NC_000913/2214458‑2214736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑