Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,859,332 T→G 9.3% L747R (CTC→CGC)  mutS → DNA mismatch repair protein MutS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,859,3320TG 9.3% 108.1 / 2.5 48L747R (CTC→CGC) mutSDNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base G (4/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

TACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCG  >  NC_000913/2859196‑2859473
                                                                                                                                        |                                                                                                                                             
tACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGc                                                                                                                                         <  1:1040262/143‑1 (MQ=255)
   gATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCGAGATGCAcg                                                                                                                                      <  1:514621/143‑2 (MQ=255)
    aTGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTg                                                                                                                                     <  1:589592/143‑1 (MQ=255)
      ggTCTGTGGCGGGCGTGGGCGTGCGCGGAAAATCTGGCGAATGAGATTAAGGCATTGACGTTATTTGCTACCCACGATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGa                                                                                                                                   <  2:408026/143‑1 (MQ=255)
                  gcgtgggcgtgCGCGGAAACTCTGGCGAATAAGATTAAGGCATTGACGTGATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGacac                                                                                                                       <  1:598955/143‑1 (MQ=255)
                     tgggcgtgCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAt                                                                                                                    >  2:599649/1‑143 (MQ=255)
                                      tCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGGTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCGCGGCGACACCATTGCCTTTGTGCACAGCg                                                                                                   >  1:582533/1‑143 (MQ=255)
                                        tGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCGCGATGCACTGGAGCCCGGGCACACCATTGCGTTTGTTCACAGCGTg                                                                                                 >  2:1025468/1‑143 (MQ=255)
                                          gCGAATATGATTAAGGCATTGACGTTATTTGCTACCCACTAGTGCGAGCTGACCCAGTTACCCGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCa                                                                                               <  2:371316/143‑1 (MQ=255)
                                               tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATg                                                                                          >  1:494419/1‑143 (MQ=255)
                                               tAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGGTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAGTGCCTTGACGCCGAGCGTgtgagatc                                                                                          >  2:518353/1‑136 (MQ=255)
                                                 aGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGATCTGGCCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGgc                                                                                        >  1:203067/1‑143 (MQ=255)
                                                    ttAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGAGCCAGTTACGGGAGAAAATGGAAGGCGTCGCTAAGGTGGATCGCGATGCACTGGAGCAGGGCGACACAAGTGGATTTATGCAGAGCGTGCGGGATcgcgcg                                                                                     >  2:255063/1‑143 (MQ=255)
                                                        ggCACTGACGTTATTTGCCACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGa                                                                                 <  1:268725/143‑1 (MQ=255)
                                                           aTTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGCCACCCTTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCa                                                                              >  1:825453/1‑143 (MQ=255)
                                                              gACTTTATTTGCTACCGACTATTTCGAGCTGAGCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGACTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCaaaa                                                                           <  2:585168/143‑1 (MQ=255)
                                                                     tttGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGAGGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGAGTGCAGGATGGCGCGGCGAGCAAAAGCtcagg                                                                    >  1:652140/1‑139 (MQ=255)
                                                                     tttGCTACCCACTATTCCGAGCTGACCCAGTTACCGGAGAAACTGGAAGGCGTGGCAAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACgg                                                                    <  2:370088/143‑1 (MQ=255)
                                                                       tGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGCAGGCCTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGCGCAAAAGCTACGGcc                                                                  >  2:140271/1‑143 (MQ=255)
                                                                        gCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCCCGGCGTGCAGGATGGCGCGGCGGGCAGAAGCTACGGCCt                                                                 >  2:725634/1‑143 (MQ=255)
                                                                          tACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTgg                                                               >  1:175500/1‑143 (MQ=255)
                                                                            cccACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAACAGCTACGGCCTGGCg                                                             >  2:398115/1‑143 (MQ=255)
                                                                            cccACTATTTCGAGCTGACCCAGTTACCGAAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCACTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCg                                                             <  1:895182/143‑1 (MQ=255)
                                                                             ccACTATTTCGAGCTGACCCAGTTACCGGAGCAAATGGCAGGCGTCGCTCACGTGCATCTCGATGCACTAGCGCACGTCGACACACTTGCCCTTATGCGCCGCGTGCAGGATGGCGCGGCCAGCCAACTCTACGGCCTGCCgg                                                            >  2:286831/1‑143 (MQ=255)
                                                                             ccACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGCGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCgg                                                            >  1:226423/1‑143 (MQ=255)
                                                                                 tATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGACGAGCAGAAGCTACGGCCTGGCGGttcc                                                        >  2:42089/1‑141 (MQ=255)
                                                                                  aTTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCa                                                       <  1:593493/143‑1 (MQ=255)
                                                                                  aTTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCAGTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCa                                                       <  1:666303/143‑1 (MQ=255)
                                                                                   tttCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCGCTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGCGCAACAGCTACGGCCTGACGGGTGCAg                                                      >  2:382164/1‑143 (MQ=255)
                                                                                   ggtCGAGCTGACCCAGTTATCGGAGAGAATGGAAGGCGTGGCTAGCGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAg                                                      <  2:168453/141‑1 (MQ=255)
                                                                                     tCGAGCTGACCCAGTTACCGGAGAAAATAGAAGGCGTAGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGct                                                    >  1:450450/1‑143 (MQ=255)
                                                                                       gcgCTGACCCCGTTCCCTGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGctct                                                  <  2:787684/141‑1 (MQ=255)
                                                                                        aGCTGACCCTGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCTCGGCGTCACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTg                                                 <  2:922996/143‑1 (MQ=255)
                                                                                               ccAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCCGCTCTGGCAGGCg                                          >  1:944816/1‑143 (MQ=255)
                                                                                               ccAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCg                                          <  1:1025468/143‑1 (MQ=255)
                                                                                                  gTTCCCGGCGAAACTGGAAGGCGTCGCTACCGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGCGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGc                                       <  1:51658/143‑1 (MQ=255)
                                                                                                  gTTACCGGTGAAGATGGAAGGCGTCGCTACCGTGCATCTCGATGCACTGGAGCACGGCGACAACCCTGCCTATATTCACAGCGTGCAGGATGGCGCGGCGAGCAAATGCTACTGTCGGGCGGTTGCAGCTCTGGCAGGCGTGc                                       <  2:319137/143‑1 (MQ=255)
                                                                                                     gccGGTGAAGATGCAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCaa                                    <  2:114486/142‑1 (MQ=255)
                                                                                                     agcGGAGAATAGGGAAGGCGTGGCTAACGTGCATCTCGATGCACTGGGGCACGGCGACACCATTGCCTTTATGCACAGGGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCaa                                    <  2:799223/141‑1 (MQ=255)
                                                                                                         gagaAGAGGGAAGGCGGCGCTAACGTGCATCGCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAaga                                <  1:109774/143‑1 (MQ=255)
                                                                                                         gagaAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGGTGCAGCTCTGGCAAGCGTGCGAAAaga                                >  2:113933/1‑143 (MQ=255)
                                                                                                         gagaAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTACGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCCGTCGCAGGTGTGGCAGGCGTGCCaaaaac                                >  2:135151/1‑141 (MQ=255)
                                                                                                              aaTGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAGGAGGttg                           >  1:880813/1‑142 (MQ=255)
                                                                                                                tGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTGTGGCAGGCGTGCCAAAAGAGGttatt                         <  1:844875/143‑1 (MQ=255)
                                                                                                                      gCGTCGCTAACGGGCGTCTCGGTGCACTGGGGCACGGCGACACCATTGTCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCACAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAgcgc                   <  2:651975/143‑1 (MQ=255)
                                                                                                                           gCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACg              >  1:357562/1‑143 (MQ=255)
                                                                                                                             tccagTGCATCGAGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCCGCTCTGGCAGGCGTGCCCAAAGCGGTTATTAAGCGCGCACGGc            >  1:427172/5‑143 (MQ=255)
                                                                                                                             tccagTGCATCGAGATGCACTGGAGCACGACGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAACAGAGGTTATTAAGCGCGCACGGc            >  1:730935/5‑143 (MQ=255)
                                                                                                                                 gtccttctcGATGCACTGGCGCACGGCGACACCATTGCCGTTATGCACGGTGTGGAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCaaaa        <  2:573439/138‑1 (MQ=255)
                                                                                                                                      tctcGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGc   <  1:354563/143‑1 (MQ=255)
                                                                                                                                       ctcGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAACGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTGTTAAGCGCGCACGGCACAAGCTGCg  >  1:277714/1‑143 (MQ=255)
                                                                                                                                       ctcGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAGGAGGGTATTAAGCGCGCACGGCGAAAGCTGCg  >  2:615510/1‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                             
TACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTATTTCGAGCTGACCCAGTTACCGGAGAAAATGGAAGGCGTCGCTAACGTGCATCTCGATGCACTGGAGCACGGCGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCG  >  NC_000913/2859196‑2859473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑