Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	153.1 / NA	52	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/28);  total (24/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTG  >  NC_000913/3041262‑3041537                                                                                                                                         |                                                                                                                                            ctctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCCCTCACGCTGATGCCGCGTGCTGACTGGGCGCGTGccgc                                                                                                                                       >  2:560443/1‑141 (MQ=255)         ccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTg                                                                                                                               <  2:717680/143‑1 (MQ=255)             aaGTCATTAATACGCCGTTAAGCAACTCAGGACGCTATTATGGCTCTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTGCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                                           <  1:631526/142‑1 (MQ=255)                  aTTAATACGCCGTTGAGCAACTCAGGCCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTCCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                      <  2:209685/143‑1 (MQ=255)                  aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGGCGCCGATGACGCTTCATGAACGGGCGCGTGCCACCGTTCCTGGCGCgggcg                                                                                                                      >  2:260302/1‑140 (MQ=255)                    tAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTCCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGc                                                                                                                    <  1:667747/143‑1 (MQ=255)                       tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaa                                                                                                                 <  1:968857/143‑1 (MQ=255)                       tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaa                                                                                                                 <  1:763080/143‑1 (MQ=255)                          gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaaa                                                                                                              <  1:51816/143‑1 (MQ=255)                           ccGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGCACAGCGAAAAat                                                                                                             >  2:47859/1‑143 (MQ=255)                              ttAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatata                                                                                                          <  1:380904/143‑1 (MQ=255)                                  gCAACTCCGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGCTTACCGCGGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCa                                                                                                      <  1:812767/143‑1 (MQ=255)                                   cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg                                                                                                     >  1:5175/1‑143 (MQ=255)                                     aCTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGCATACTGCGGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGGCAGCGAAAAATATATCCAggg                                                                                                   >  1:952924/1‑143 (MQ=255)                                         aGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGCTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAGGATATGTGCAggggcgg                                                                                               >  1:271039/1‑139 (MQ=255)                                           gACTCTATTATGGCTTTTACTCGTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAGGTAGGTGCAGGGTCgggg                                                                                             >  2:932746/1‑142 (MQ=255)                                             ctctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa                                                                                           >  1:982773/1‑143 (MQ=255)                                                tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcag                                                                                        <  1:617270/143‑1 (MQ=255)                                                  ttatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                                                                      <  2:677386/143‑1 (MQ=255)                                                     tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                                                                   >  2:287027/1‑143 (MQ=255)                                                     tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCACGGACAGCGAAAAATAAATACAGGGAAAGGTGACAGCAGAt                                                                                   >  1:627135/1‑143 (MQ=255)                                                               acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGGTGATCCCGCGTGATGCCTGGGCGCGGGCCACCACTACTGGCGCGGACGGCGCACACTAGGGGCAGCGACACGTGGCAGCAGATGGCAGcccgc                                                                         >  2:284086/1‑140 (MQ=255)                                                               acCTTTTCCTCCCCGTCAGCCTACGGCGTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGa                                                                         <  1:673594/143‑1 (MQ=255)                                                                      cctccCCGTGAGCCGACGGCTTCTGCCCGTGTACCGCTGACGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCaga                                                                  <  2:653768/143‑1 (MQ=255)                                                                           ccGTCAGCCTCCGGCTGCGGAAAGTCTCCCTCTGACGCTGATGAGGTTTGATTACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                             <  1:947242/143‑1 (MQ=255)                                                                              atatccTACTGCTTGTGCGCGTTTACCGTTGACGCTGATGACGCTTGATGACTAGGCGCGTGACACCATTACTTTCATGGACAGCGAAAAATATATGCAGGGTCAGGTTACAGCAGATGTCAGCCAGATGGCAGAAGATCAGc                                                          <  2:126314/139‑1 (MQ=255)                                                                                aGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTCACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAc                                                        <  1:684847/143‑1 (MQ=255)                                                                                  ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCt                                                      >  1:594497/1‑143 (MQ=255)                                                                                  ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGTATGCGCGCAATATATCCAGGGTCAGGTGACAGCAGCTGTCAGACAGATGGCAGAAGAGCAGCACCt                                                      >  2:122939/1‑143 (MQ=255)                                                                                    tACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGGTGTTATCCGGACGGCAGGAGATCAGCACCTGc                                                    >  1:192382/1‑143 (MQ=255)                                                                                     aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGAAGCTTGATGACTGGGCGCGTGCCACCATTAATGGCGCGGACAGCGAAAAATATATGCAGGGAAAGGAGACAGCAGATGTCAACCAGATGGCAGAGGCACAGCACATGCt                                                   >  2:350176/1‑143 (MQ=255)                                                                                       ggCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATAAGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcg                                                 <  1:629950/143‑1 (MQ=255)                                                                                        gCTTCTGCCCGTTTACCGCTGACGCTGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTGAGCCCGATGGCAGACGCTCAGCACCTGCAcgg                                                >  2:386659/1‑142 (MQ=255)                                                                                          ttCTGCCCGTTTATCGCTGTCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTACTcgccg                                              <  1:781135/143‑1 (MQ=255)                                                                                          gtCTGCCCGTGTACCGCGGACGCTGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg                                              <  1:586904/142‑1 (MQ=255)                                                                                             tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCAAGATGGCAGAAGATCAGAAAAAACTCGCCGccc                                           >  2:162253/1‑143 (MQ=255)                                                                                                ccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCaat                                        >  2:152219/1‑141 (MQ=255)                                                                                                                 tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGCTCAGCACCTGCTCGCCGCCCACTGCGACCCCAAAAGtgac                       >  2:401639/1‑140 (MQ=255)                                                                                                                  gatgaCGCTTGATGGGGGGGCGCGTGCCGCCAGTGCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaaa                      <  1:185051/143‑1 (MQ=255)                                                                                                                   cggaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAt                     <  2:586760/141‑1 (MQ=255)                                                                                                                   atgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAt                     <  2:704967/143‑1 (MQ=255)                                                                                                                      acggatgaagaCTGGCCGCGTGCCAACATTAGTGGCGCGGACAGCGAAAAATAAATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAGCTGTTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgtg                  <  2:507002/138‑1 (MQ=255)                                                                                                                       gtctggatgaCGGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAACAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg                 <  1:250724/138‑1 (MQ=255)                                                                                                                        gCTTGATGGCTGAGCGAGTGCCCCCATGACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGGGACAGCAGATGTCAGGCAGATGGAAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa                <  1:114953/143‑1 (MQ=255)                                                                                                                        gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa                <  1:287027/143‑1 (MQ=255)                                                                                                                        gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCAGACAGCGAAAAATAGATGGAGGGTCAGGTGACAGAAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCAGCCCATTGCGACGCCACAGGTAAAATGTGGa                >  1:868229/1‑143 (MQ=255)                                                                                                                           tgatgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGCAGAGCAGCACCTGCTCGCCGCCCACTGAGGTGAAAAAGGAAAAATGAGAAGcc             >  1:188830/1‑142 (MQ=255)                                                                                                                               ggcggggCGCGGGCCACCATTACTGGCGCGGAGAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAttt         <  1:859162/139‑1 (MQ=255)                                                                                                                                  tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGc                                      <  1:984093/111‑1 (MQ=255)                                                                                                                                  tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGc                                      >  2:984093/1‑111 (MQ=255)                                                                                                                                  tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGGGGAGCAGTTTACg      >  2:568630/1‑143 (MQ=255)                                                                                                                                     gcgcGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCACAGGTAAAATGTGGAGCAATTTACGtgt   >  2:1045206/1‑141 (MQ=255)                                                                                                                                      cgcgTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTGGCCGCCCCATCCGACGCCAAAGGTAAAATGTGGAGCAACTTACGTCTg  >  2:467697/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                            CTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTG  >  NC_000913/3041262‑3041537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑