| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 282,518 | G→A | 7.3% | G81S (GGC→AGC) | yagE → | CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 282,518 | 0 | G | A | 7.3% | 143.0 / 3.4 | 57 | G81S (GGC→AGC) | yagE | CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase |
| Reads supporting (aligned to +/- strand): ref base G (25/27); new base A (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-02 | |||||||||||
CGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAG > NC_000913/282376‑282655 | cgacgcTCTGGTCAAGGCAGGCGTTGACGGCCTGTTCTGCCTGGGGAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGCTCCTGTCGATCGTCGCGTGCCGGTGCTGATCg < 2:712306/143‑1 (MQ=255) gaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGGTCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAc < 2:178934/143‑1 (MQ=255) aTCTGATCTAAGCTTGCGTTTACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAcc < 1:345041/143‑1 (MQ=255) cTGATCAATGCAGGCGTTGACGCCGTGTTTTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcgg < 2:972859/143‑1 (MQ=255) tGATCAAAGCAGGCGTTGACGGCCTGTCCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc < 1:708001/143‑1 (MQ=255) gATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGAACATGTCGATCGTCGCGTGCCGCTGCTGCTCGGCCCcggcg > 2:89136/1‑143 (MQ=255) aTCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcggcgg > 1:843046/1‑143 (MQ=255) aaaGCAGGCGTTGACGGCCTGTCCGTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCAc < 2:721009/143‑1 (MQ=255) ggcgggCGCTGACGGCCTGTTCTGCCTGGGCAGCGGGGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGGCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCa < 1:381968/139‑1 (MQ=255) cgCAGGCGTTGACGGCCCGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGGTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCa < 1:1023449/142‑1 (MQ=255) cAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAAc < 1:838576/143‑1 (MQ=255) cAGGCGTTGACGGCCTGTTCTGCCTGGGCAGGGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAAc < 2:530433/143‑1 (MQ=255) gCGTTGGCGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGcc < 1:383457/143‑1 (MQ=255) gTTGACGGCCTGTTCTTCCTGGGCAGAGATGGCGAGTTCGCCCGGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTGTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCCCCGGCGGCACCAACACCCg > 1:730944/1‑143 (MQ=255) ttGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGTCCgg < 2:538251/143‑1 (MQ=255) agggCCTGGTCTTCCTGGGCAGCGGTGGCGAGTGCTCCCGGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTAGCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGaa < 2:825708/141‑1 (MQ=255) ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGCGCTGATCGGCACCGGCGGCACCAACGCCCGGCAAAc > 2:37642/1‑143 (MQ=255) ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTACCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc > 1:611271/1‑143 (MQ=255) ggCCTGTTCGTCCTGGGCAGCGGTGGGGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc < 2:139339/143‑1 (MQ=255) cTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAccct > 1:84701/1‑141 (MQ=255) cTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCAt < 1:866084/143‑1 (MQ=255) cttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTTGCGATCATGTCGCTCGTCTCGTGCCGGTGATGATCGGCAAAGGCGGGAACAACGATCGGGAAAACATCggaa > 1:342886/1‑140 (MQ=255) cttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAGAGGAGGCACCACCGCCCCGGAAACCATCGAAc > 2:215111/1‑143 (MQ=255) ttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACt < 1:870218/143‑1 (MQ=255) ccggggCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCAGTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTca < 2:592560/140‑1 (MQ=255) tGGGTAGCGGTGGCTAGTTCTCCCAGCTCGGCCCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcagc < 1:761585/143‑1 (MQ=255) tGGGCAGCGGTGGCGAGTTCCCCCAGCGCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCAGGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcagc < 1:740970/143‑1 (MQ=255) gCGGTGGCGAGTTCTCCCCGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCAc < 2:289889/143‑1 (MQ=255) tGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAACGTCGCGTGCCGGTGCTGATCCGCCCCGGCTGCACCAACGCACGGGACACCATCGGACCAAACCAGCAcgcac > 2:296512/1‑141 (MQ=255) cGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCgcagc > 1:79428/1‑143 (MQ=255) ccAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCAGCAACGCCCGGGAAGCCACCGACCTCAACCCGCACCCGCCACAggcgggcg > 2:65448/1‑143 (MQ=255) ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCGGAGGCATCTTTCGAACTCTGCCAGCACGCGCCGCAGGCGGGCGCGGACgg > 1:289404/1‑143 (MQ=255) gcgcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCGTCGAACTCCGCCAGCACGCGCAGCAGGCGGGCGAGGCCGGc > 2:281875/1‑143 (MQ=255) gcgcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTACTGATCGGCACCGGCGGCACCCACGCCCGGGAAACCCTCGGACTCCGCCCGCACGCGCAGCAGGCGGGCGCGGACGGc > 2:481734/1‑143 (MQ=255) gcCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGCACTCAGCCAGCACGCGCGGCGGGCGCGGGCGGACGGCAt > 1:220593/1‑143 (MQ=255) gagCGTAAAGCCATGGCCCGCGTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCAGCGGCACCAACGCCCGGGAAACCATCGAGCTCAGCCAGCACGCGCAGCCGGTGGTCGCGGACGGCATCgtggt < 2:647614/143‑1 (MQ=255) gCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGa < 2:197779/143‑1 (MQ=255) tAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCTGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGAGGGCGCGGACGGCATCGTGGTGATCa > 1:247231/1‑143 (MQ=255) tAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGCTCGGCACCGGCGGCACCAACGCCCGGGCAACCATCGGGCTCAGCCAGCACGCGCAGCAGGCGGGCGCGCACGGCGTCgcggtgcgca > 2:617961/1‑139 (MQ=255) aaaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGCTCGGCACCGGCGGCACCAACGCCCGGGAGACCCTCGGGCTCAGCCAGCCCGCGCAGCAGGCGGGCGCGGGCGGCATCgggggggacaa > 1:25993/1‑136 (MQ=255) aTTGGCCGCTGTGCTGTCGCTCGTGTCGATCGTGGCGTGCCGGTGTTGGTCGGCACCGGCGGCACCAACGCCCGGGACACCATGGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCcta < 2:545222/143‑1 (MQ=255) cTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCACCGCCCGGGACACCTTCGAACTCAGCCAGCACGCACAGCAGGAGAGCACGGACGGCATCATAGGGATCAACAGATACTACTgaa > 1:322172/1‑141 (MQ=255) tttGCTATCGGTCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaa < 1:602454/143‑1 (MQ=255) tttGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCATAATAATGGac > 1:765008/1‑142 (MQ=255) ttGCTAGCGAGCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGaaa < 2:662293/143‑1 (MQ=255) atcgatcgTGCCGATCTTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTc < 2:731357/143‑1 (MQ=255) tCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCCGCACGCGCAGCAGGCGGGCGCGGCCGGCATCGTGGTGATCAACCCCTCCTACTGGAAAGTGTCGGagg > 1:1033944/1‑141 (MQ=255) tCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAg < 1:916509/143‑1 (MQ=255) tCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCGGCAGGCGGGCGCCGACGGCATCGTGGTGACCCACCCCGACTACCGTAAAGGGTGCGAAggggac > 2:555188/1‑138 (MQ=255) gtcgCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATc > 1:203479/1‑143 (MQ=255) cgcgTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCg < 1:993738/143‑1 (MQ=255) cgTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCCTCGGGGTGATCAACCCCTACGCCTGGGAAGGGACGGAAGCGCAACTGacgcgcc > 2:658655/1‑137 (MQ=255) cgCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGAGCAACCCCTACCACTGGAAAGTGTCGGAAGCGACCCTGATCCGccac > 2:160932/2‑140 (MQ=255) cgCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGACGGGGTTGGCATCCTGGAGAGCAACCCCTACTACTGGAAAGTGTCGGAAGCGACCCTGGTGCGCCAt > 1:289760/2‑143 (MQ=255) gcGGTGCTGATCGGCACCGGCGGCACCAACGGCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTAttt < 2:569811/142‑1 (MQ=255) cTGATCGGGACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCa < 2:414753/143‑1 (MQ=255) cTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTCCTGGAAAGTGTCGGAAGCGAACCTGATCCGCTGTTTCGAgta > 1:135700/1‑141 (MQ=255) tGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGAGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGGAGCGAACCTGATCCGCCATCTCGAGCAg > 2:529894/1‑143 (MQ=255) | CGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAG > NC_000913/282376‑282655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |