BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,900,410	A→T	6.6%	P56P (CCT→CCA)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,410	0	A	T	6.6%	326.1 / 11.3	134	P56P (CCT→CCA)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand): ref base A (69/51); new base T (4/5); total (76/58)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 137 total aligned reads displayed.

CATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCG  >  NC_000913/3900268‑3900552

cATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGCCTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGACCACCCACCACTACAGAGACATTTTTTGCCCCa                                                                                                                                                 >  1:855142/1‑143 (MQ=255)

 aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAg                                                                                                                                                >  2:957623/1‑143 (MQ=255)

   cAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGGCACGACCGGTCTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAggg                                                                                                                                              <  2:714852/143‑1 (MQ=255)

     actttGCAAACATACTCGTACAGGTTGCGTTTCAGTATAGGCGTGCGCCACGACCAGTCTCCTCCTCCGACTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACGCCCACCACTACTGAGACATTTTTTGCCCCTGGGGc                                                                                                                                            <  2:862694/140‑1 (MQ=255)

     aCATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTGGGGc                                                                                                                                            <  2:377760/143‑1 (MQ=255)

     aCATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATACTGTCCGGAACCGGCACACCCCCCACTACAGAGACATTTTTGGCCCCAgggcc                                                                                                                                            >  2:548232/1‑141 (MQ=255)

      cATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCa                                                                                                                                           <  1:122592/143‑1 (MQ=255)

         tGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGAGATTTTTTGCCGCAGGGGCcaat                                                                                                                                        >  2:1055408/1‑142 (MQ=255)

           aaaaaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGAATCCTGCTTCATCGTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATCTTGTGCCCCAGGGGCAAAG‑Ta                                                                                                                                     >  2:700289/1‑143 (MQ=255)

              aaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAg                                                                                                                                                <  2:324400/130‑1 (MQ=255)

              aaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAg                                                                                                                                                >  1:324400/1‑130 (MQ=255)

               aaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCGTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGa                                                                                                                                 <  1:549386/143‑1 (MQ=255)

                acaCTCGTACAGGTCGCCCTTCAGGATAGGTGTGCGCCACGCCCCGACTCCTGCTTCGTCTTTGGTCATCGGGTGACTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGaa                                                                                                                                <  1:807011/141‑1 (MQ=255)

                    tCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGCGAATATTGTCCGGACCCGGAACACCCACCAGTACAGAGACACTTTTTGCACAAGAGGCCAAG‑TAACGAAGGGt                                                                                                                            >  2:341071/1‑143 (MQ=255)

                    tCGTACAGGTTGCCTTTCAGGATAGGGTTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAGTTCGTGCCCCAGGGGCAAGG‑GAGCGAAAGGt                                                                                                                            >  2:67816/1‑143 (MQ=255)

                     cttACGGGTTGCCGTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGACAAAG‑TAGCGAAAGGTc                                                                                                                           <  2:280097/141‑1 (MQ=255)

                      gTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCa                                                                                                                          <  1:425506/143‑1 (MQ=255)

                       tACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCAc                                                                                                                         >  1:792234/1‑143 (MQ=255)

                       tACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTGTGCCCCAGGGACAACG‑AACCGAGAGGTCAc                                                                                                                         >  1:921007/1‑143 (MQ=255)

                            gTTGCCTTTCCGGATACGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGt                                                                                                                    <  1:429278/143‑1 (MQ=255)

                            gTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGt                                                                                                                    >  1:944001/1‑143 (MQ=255)

                             ttGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGtt                                                                                                                   >  1:838560/1‑143 (MQ=255)

                              tGCCTGGCAGGACAGGTGTGGGCTACGCCCAGGCTCCTTCTGCGTCGTGGGGCATCGGGTGAATATTGTCCGGAACCGGAAGACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTa                                                                                                                  <  2:792741/143‑1 (MQ=255)

                                ccTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGCACACCCACCGCTCCAGAGCCATTTTTTGCCACAGCGGTAAAT‑TCGGGAGTGGGCACGCCGTTATc                                                                                                                >  2:743192/1‑143 (MQ=255)

                                ccTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATc                                                                                                                <  2:651770/143‑1 (MQ=255)

                                 cTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGGCTTTGGTCATCGGGTCACTATTGTCCGCAACCGGAACACCCCCCACGGCAGAGACATGCTTCGCGCCAGGCGCCCAG‑TAGCGAACGGGCACGGTGGTCTCg                                                                                                               >  2:276904/1‑143 (MQ=255)

                                  tttCAGGATAGGTGTGCGCCACGACCATACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCgg                                                                                                              <  2:557141/143‑1 (MQ=255)

                                  tttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCGCAGGGGCGAAG‑TAGCGAAAGGGCACGCTGTTATCgg                                                                                                              >  1:18891/1‑143 (MQ=255)

                                   ttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCggg                                                                                                             >  1:422908/1‑142 (MQ=255)

                                       ggATAGGTGTGCGCCACGACCACACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTg                                                                                                         <  1:136243/143‑1 (MQ=255)

                                        gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGGACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGa                                                                                                        >  1:723497/1‑143 (MQ=255)

                                        gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGCCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGa                                                                                                        <  1:57141/143‑1 (MQ=255)

                                          tAGGTGTGCGCCACGACCAGCCTCCTGCTGCGTCTTTGGTCATCGGGTGAAGATTTTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGAcc                                                                                                      <  2:975605/143‑1 (MQ=255)

                                          tAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGAcc                                                                                                      <  1:696550/143‑1 (MQ=255)

                                           agttgtgCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                                                                     <  1:248489/140‑1 (MQ=255)

                                             gtgtGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGa                                                                                                   >  1:463471/1‑143 (MQ=255)

                                              tgtgCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAg                                                                                                  >  1:723083/1‑143 (MQ=255)

                                                ctcgcCTCGGCCATGCTCCTGCTTCGTCTTTGGGCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGCGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTc                                                                                                <  2:1057736/141‑1 (MQ=255)

                                                 gcgcCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTca                                                                                               <  1:783501/143‑1 (MQ=255)

                                                   gcCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACCGAGACATTTTTTCCCCCAGGGGCACAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGATTcaca                                                                                             >  2:111008/1‑143 (MQ=255)

                                                    ccACGACCAGTCTCCTGCTTCGTCTTTGGTCATCGGGTGACTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTCTGCCCCTGGGGCAAAT‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACAt                                                                                            <  1:695673/143‑1 (MQ=255)

                                                    ccACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACAt                                                                                            >  1:120968/1‑143 (MQ=255)

                                                     cACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGCGACATTTTTTGCCCCAGGGGCACAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGGCACATa                                                                                           >  2:345708/1‑143 (MQ=255)

                                                         tccAGACGCCTGCTTCGTCTTTGGTCAGCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGt                                                                                       <  2:446764/142‑1 (MQ=255)

                                                           cAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGCCCGGAGACACATACTGttt                                                                                     >  2:852152/1‑143 (MQ=255)

                                                               cTCCTGCTGCGGCTTTGGGCAGCGGGTGAAGATTGTCCGGATCCGGAGCACCCACCACTACAGAGACATTTTTTGCCCCAGTTGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCAcc                                                                                 <  2:696099/143‑1 (MQ=255)

                                                                tCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGCACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCGGAGTCCCCTACTGGTTCGCCg                                                                                >  2:854830/1‑143 (MQ=255)

                                                                tCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGCCACATACTGTTTCACCg                                                                                >  2:13875/1‑143 (MQ=255)

                                                                 cctggggcGTCTTTGGCCATCGGGGGAAGATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGGAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCgg                                                                               <  2:681997/136‑1 (MQ=255)

                                                                 ccTGCTTCGTCTTGCGTCACCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCgg                                                                               <  2:951981/143‑1 (MQ=255)

                                                                  ctccTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCggg                                                                              <  1:188026/140‑1 (MQ=255)

                                                                  cTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGCAACAGAACACGCAACACTACCGAGACATTTTTTGCCCCAGGGGCCACG‑TAGCGCAAGGTCACGCTGTCCTCGGCACTGACCTGAGCCCCATACTATCTCACCgcg                                                                              >  2:227422/1‑141 (MQ=255)

                                                                  cTGCTTCGTCTTTGGTCATCGGGTGAATATTATCCGGCACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCACAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCCCCggg                                                                              >  1:294280/1‑143 (MQ=255)

                                                                     cTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCGTTGACCTGAGCGACATCCTGTGTCACCGGGATa                                                                           >  2:388396/1‑143 (MQ=255)

                                                                      ttCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCCGCCCCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAg                                                                          >  1:778663/1‑143 (MQ=255)

                                                                       tCGTCTTTGGTCATCGGGTGAATATTGCCCGGAACCGGAACACCCACCACTACAGAGAAATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTGCACAGGGATAGt                                                                         >  1:895248/1‑143 (MQ=255)

                                                                        cGTCTTTGGTGAGCGGGTGAGTATTGTGCGGAACCGGAGCACCTACCACTACAGAGACATTTTTTGCCCCGGGGGCGAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACGGTTTCAAGGGGATGGTg                                                                        >  2:974288/1‑143 (MQ=255)

                                                                        cGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTTGGGCAACA‑TAGCGTGAGTTTACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTg                                                                        <  1:446464/143‑1 (MQ=255)

                                                                        cGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTg                                                                        <  1:957623/143‑1 (MQ=255)

                                                                         gTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGTGCAAGGTCACGCTGTTATCCGCATTGACCTGAGCCACATCCTGTTTCACCGGGATAGTgg                                                                       >  2:199852/1‑143 (MQ=255)

                                                                           cTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGt                                                                     >  2:871047/1‑143 (MQ=255)

                                                                            tttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTg                                                                    >  2:225974/1‑143 (MQ=255)

                                                                               ggTCATCCTGTGAATATTGCCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCg                                                                 <  2:663335/143‑1 (MQ=255)

                                                                                 tCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCggg                                                               >  1:785906/1‑143 (MQ=255)

                                                                                   aTCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTCCGCCGGGATCGCGGGTGCCGggct                                                             >  2:202108/1‑143 (MQ=255)

                                                                                       ggTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATGGTGGGTGCCGggctggct                                                         >  1:71458/1‑143 (MQ=255)

                                                                                       ggTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctggct                                                         >  1:64960/1‑143 (MQ=255)

                                                                                        gTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑CAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGCTTCACCGGGATAGTGGGTGCCGGgctggctg                                                        >  2:225844/1‑143 (MQ=255)

                                                                                         tGAGTAGTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTGGCCCCAGGGGCAGAG‑TAGCGAAAGGTCACGCTGTTATCGGCAGTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGctggctgg                                                       <  2:287880/143‑1 (MQ=255)

                                                                                         tGAATAGTGTCCGGAACCGGAACACCCACCACTACAGAGAGATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGctggctgg                                                       <  1:1038126/143‑1 (MQ=255)

                                                                                           aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCa                                                     >  1:470642/1‑143 (MQ=255)

                                                                                             tatTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTG‑CCCACGGCCTTTGAGAGCTAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCata                                                   <  1:1055512/143‑1 (MQ=255)

                                                                                                    cggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctg                                            >  1:1052582/1‑143 (MQ=255)

                                                                                                    cggaaccggaacACCCACCACTACAGAGACATCTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGAATAGTGGGTGCCGGGCTGGCTGGCATATGCgctg                                            >  2:556195/1‑143 (MQ=255)

                                                                                                            gaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGAATAACGTCTGCTATGGca                                    >  1:656786/1‑142 (MQ=255)

                                                                                                              ccgcccaccACTACAGTGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCa                                  <  2:858948/140‑1 (MQ=255)

                                                                                                              acacCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCa                                  <  2:443879/143‑1 (MQ=255)

                                                                                                                agccaccaCTACATAGCCATTTTTTGTCCCAGGGGCAAAG‑TAGCGACAGGTCACGCTGTTATCTGCGTCGCTTTGTTTCACATCCTCTTTCACCGGGATAGTGGGTGCCGGGCTGTCTGGCATATCCGCTGCTATGGCCCACt                                <  2:641254/141‑1 (MQ=255)

                                                                                                                acccaccACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACt                                >  1:834472/1‑143 (MQ=255)

                                                                                                                  ccaccaCTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGATGATATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGa                              >  1:670307/1‑143 (MQ=255)

                                                                                                                   caccacTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAg                             >  1:963933/1‑143 (MQ=255)

                                                                                                                   aaccacTACAGGGACAGTTTTGGCCCCAGGGGCAAAG‑TAGCGAAGGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCTGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAg                             <  2:771029/142‑1 (MQ=255)

                                                                                                                     ccacTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATCTCCGCTGCTATGGCCCACTGCGCa                           >  2:451891/1‑143 (MQ=255)

                                                                                                                     ccacTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCa                           >  1:851509/1‑143 (MQ=255)

                                                                                                                      cacTCCAGAGACATTTTTGGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAg                          <  1:476184/143‑1 (MQ=255)

                                                                                                                      cacTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAg                          >  1:1953/1‑143 (MQ=255)

                                                                                                                      cacTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTCGCTGGCATATCCGCTGCTATGGCCCACAGAGCAg                          >  2:1302/1‑143 (MQ=255)

                                                                                                                      cacTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTCGGTGCCGGGCTGGCTGGCATATCCGCTGCTCGGGCCCACGGAGcgg                          >  1:327813/1‑141 (MQ=255)

                                                                                                                       acTACAGGGACATGTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGa                         <  2:783672/143‑1 (MQ=255)

                                                                                                                       acTACAGAGACATGTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCGGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGa                         <  1:801636/143‑1 (MQ=255)

                                                                                                                              gggcATTTTTTGCCCCAGGGGCAAAG‑TATCGCAAGGGCACGCTGTTATCGGCGTTGCCCTGCGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCg                  <  2:956491/140‑1 (MQ=255)

                                                                                                                              agaCATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCg                  <  2:120968/143‑1 (MQ=255)

                                                                                                                                   ttttttGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGCGCTGGCTGGCATATCCGCTGCTATGGCCCACGGAGCCGAGATACCGCTGGc             >  2:341675/1‑143 (MQ=255)

                                                                                                                                   tactttGCCCCTGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGc             >  1:57709/4‑143 (MQ=255)

                                                                                                                                   tactttGCCCCTGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGCTTCACCGGGATAGTGGCTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTTAGCCGAAACACCGccgcc             >  2:701751/4‑139 (MQ=255)

                                                                                                                                          ccccAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTATAAAAATAAACGTATGTGTAAGGCAGCATTTCACGCCCGCTGCTATGGCCCACAGAGCAGAACTACCGCGGGCAATAAcc      >  2:753391/1‑143 (MQ=255)

                                                                                                                                           cccAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGAAGAATTACAATTCGTCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCa     >  1:487765/1‑143 (MQ=255)

                                                                                                                                             cAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGc   <  1:852152/143‑1 (MQ=255)

                                                                                                                                              aGGGGCAAAG‑TAGCGAAAGCTCCCGCTGTTCTCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCg  <  1:330766/143‑1 (MQ=255)

                                                                                                                                              aGGGCCAAGG‑TAGCGAAAGGTGACCATGTTGGCGGCATTGACCTGAGTCACATACTGGTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCg  <  2:327813/143‑1 (MQ=255)

CATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAG‑TAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCG  >  NC_000913/3900268‑3900552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`