BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,900,542	A→T	7.4%	I12I (ATT→ATA)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,542	0	A	T	7.4%	384.1 / 16.5	136	I12I (ATT→ATA)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand): ref base A (57/66); new base T (4/6); total (64/72)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 136 total aligned reads displayed.

CCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGT  >  NC_000913/3900406‑3900683

ccccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTATAAAAATAAACGTATGTGTAAGGCAGCATTTCACGCCCGCTGCTATGGCCCACAGAGCAGAACTACCGCGGGCAATAAcc                                                                                                                                         >  2:753391/1‑143 (MQ=255)

 cccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGAAGAATTACAATTCGTCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCa                                                                                                                                        >  1:487765/1‑143 (MQ=255)

    atgggCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACGTGAGCAAACTCACCG‑AGCGCGGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCg                                                                                                                                     <  1:774328/141‑1 (MQ=255)

     ggggCAGAGTAGCGAAGGGTGACGCTGTTGTCGGCATTGACCTGGG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACGGAGCAGAAATACCGCTGGCTATTGCCAGCgg                                                                                                                                    <  2:990937/143‑2 (MQ=255)

      gggCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGtt                                                                                                                                   >  1:831976/1‑143 (MQ=255)

       ggCAAAGTAGCGAACGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATAACGCTGGCTATTGCCAGCGGTa                                                                                                                                  <  1:337671/143‑1 (MQ=255)

       ggCAAAGTAGCGAAAGGTCACGCTGGTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCGGTa                                                                                                                                  <  2:189502/143‑1 (MQ=255)

         cAAAGTAGCGAACGGTCACGCTGTTAGCGGCATGGACCTGAG‑TCACAGACTGTTGCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                <  1:36248/143‑1 (MQ=255)

          aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCCATGGCCCCCTAAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAag                                                                                                                               <  1:548232/143‑1 (MQ=255)

          aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGAGCTGGCTGGCATATCCGCTGCTAAGGCCCAGGGAGCAGAACTACCGCTGGCAATAACCAGCGTTAAag                                                                                                                               >  2:41656/1‑143 (MQ=255)

           aagcggCGAGAGGTCACGCGGTTATGGGCGTTGACCCGAG‑TCAGATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagc                                                                                                                              <  2:464545/138‑1 (MQ=255)

           aaGTAGCGGAGTGTCACGCTGTCATCGGCATTGCCCGGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagc                                                                                                                              <  1:871057/143‑1 (MQ=255)

            aGTAGCCAAAGGTCACGCCGTTATCGGCATTGACCTGAG‑TCACATACTGTTGCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagca                                                                                                                             <  1:472433/143‑1 (MQ=255)

              tAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGAGCTGGCTGGCATATCCGCTGCTATGGCGCACTGAGCAGAAATACCGCTGTCAAGAACCAGCGGTAAatcagc                                                                                                                           >  2:46781/1‑143 (MQ=255)

                  gAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGCGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAtt                                                                                                                       >  1:1021833/1‑143 (MQ=255)

                   aaaGGTCACGCTGTTATCGGGAGTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAttt                                                                                                                      <  2:792234/143‑1 (MQ=255)

                    aaGGTCTCGCGGTTATCGGCATTGGCCTGAG‑TCACATACTGTTTCACCGGGATTGTGTGTGTCTGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAtttt                                                                                                                     <  2:921007/143‑1 (MQ=255)

                    aaGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCCATAGCCAGCGCTAAAGCAGCAAtctc                                                                                                                     >  2:613439/1‑140 (MQ=255)

                       gTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGACATACCGCTGGCAATAGCCAGCGTCAAAGCAGCAATTTTtac                                                                                                                  >  2:496326/1‑142 (MQ=255)

                        tCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTtata                                                                                                                 >  2:1032327/1‑143 (MQ=255)

                        tCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGAAATGGCCAGCGTTAAAGCAGCAATTTGtata                                                                                                                 >  1:68743/1‑143 (MQ=255)

                          aCGCTGTCATCGGCACTGACCTGAC‑TCACATACTGTTTCACCGGGATAGTGCGTGCCGGGCTGGCTGGCATCTCCGCTGCTATGGCCCACTTAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATAtt                                                                                                               <  1:171336/143‑1 (MQ=255)

                              tGTTATCGGCATTGCCCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATc                                                                                                           <  1:1065286/143‑1 (MQ=255)

                                ttATTGGCATTGACCTGAG‑GGACATACTGTGTCACCGGGATAGTGGGTGCCGGCCTGGCTGGCTTATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCAAGAGTTAAAGCAGCAATTTTTATATTCATCGt                                                                                                         <  2:676701/143‑1 (MQ=255)

                                  aTCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGCTAAAACAGAAACTTTTAAAGTCATCGGaa                                                                                                       >  2:712378/1‑143 (MQ=255)

                                   tCGGCATTGCCCTGAG‑TCACATACTGTTTCACCGGGATCGTGGGTGCCGGGCTGCCTGGCCTATCCGCTGCTATGGCCCACTAAGCAGCAATACCGCTGGCAATCGCCAGCTGTAAAGCAGCCATTTCTATATCCACCGTCat                                                                                                      >  2:985408/1‑143 (MQ=255)

                                   tCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCAGGCCGGCATATTCGCTTGTTTGGCCCACTGAGCAGCAATACCGCGGGCAATAGCCAGCGTTAAAGCCGCGCTTTTTATATTCATCGTAat                                                                                                      >  1:669852/1‑143 (MQ=255)

                                      gCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATAtt                                                                                                   >  1:102083/1‑143 (MQ=255)

                                      gCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATAtt                                                                                                   <  1:425116/143‑1 (MQ=255)

                                      gCATTGACCTGAG‑TCACATACTGTTCCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATAtt                                                                                                   <  1:129720/143‑1 (MQ=255)

                                           gACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCttt                                                                                              <  1:255942/143‑1 (MQ=255)

                                            aCCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCGATTTTTAGATGCATCGTAATATTCCTTTc                                                                                             >  2:503707/1‑143 (MQ=255)

                                               tGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGACGAAATACCGCTGGCAATAGCCAGCGTTAACGCAGCACTTTTTATATTCATCGTAATATTCCTTTCAtt                                                                                          >  2:58232/1‑143 (MQ=255)

                                                gAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCCATTTTTATATTCATCGTACTATCCCTGTGATTa                                                                                         >  2:778557/1‑143 (MQ=255)

                                                 aG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTCAAGCAGCAATTTCTATATTCATCGTAATATTCCTTTCATTaa                                                                                        <  1:1065771/143‑1 (MQ=255)

                                                 aG‑TCACATACTGTTTCACCGGGATAGTGGGGGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTaa                                                                                        <  2:723083/143‑1 (MQ=255)

                                                     cacaTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAAGACCGCTGGCAATAGCCAGCGTTAAAGCACCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGtt                                                                                     <  1:227422/143‑1 (MQ=255)

                                                        aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGGTGGCATATCCGCTGTTATGCCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCa                                                                                  <  1:1055408/143‑1 (MQ=255)

                                                        aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCa                                                                                  <  1:871047/143‑1 (MQ=255)

                                                         tACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCACCAATTTTTATATTCATCGTAATATTCCTTTCATTCAGTTCCac                                                                                 >  2:714488/1‑142 (MQ=255)

                                                          aCTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTGATATTCCTTTCATTAAGTTCCAtt                                                                                >  1:209935/1‑143 (MQ=255)

                                                              tttCACCGGGTGAGTGGGTGCCGGGCGGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCTATTacca                                                                            <  2:294280/143‑1 (MQ=255)

                                                              tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTaccc                                                                            >  1:630542/1‑142 (MQ=255)

                                                              tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTacca                                                                            >  1:701195/1‑143 (MQ=255)

                                                              tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTacca                                                                            >  1:330454/1‑143 (MQ=255)

                                                                 cACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTAccacca                                                                         >  1:559326/1‑143 (MQ=255)

                                                                  aCCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATGTTCCTTTCATTAAGTTACAATaccacccg                                                                        >  2:307598/1‑141 (MQ=255)

                                                                          aGTGGGTGCCGGGCTGGCTGGCATAGCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATGCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGc                                                                <  1:503875/143‑1 (MQ=255)

                                                                           gTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGcc                                                               <  1:861187/143‑1 (MQ=255)

                                                                           gTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTCCAGTTAGTTCCATTACCACCAGATTTCAGcc                                                               >  1:1001003/1‑143 (MQ=255)

                                                                            tGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCt                                                              <  2:24806/143‑1 (MQ=255)

                                                                             gggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAACTACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTACTATTCCTTTCATTACGTTCCATTACCACCAGATCTCCGCCTg                                                             >  1:163723/1‑143 (MQ=255)

                                                                             gggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCCGATTTCAGCCTg                                                             >  1:280894/1‑143 (MQ=255)

                                                                             gggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTg                                                             <  2:711247/143‑1 (MQ=255)

                                                                               gggCCGGGCTGGCTGGCATAGCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                           <  1:199852/141‑1 (MQ=255)

                                                                               gTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                           >  1:8618/1‑143 (MQ=255)

                                                                                tGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTCAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggac                                                          >  1:595818/1‑141 (MQ=255)

                                                                                tGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGc                                                          >  1:251345/1‑143 (MQ=255)

                                                                                       ctggctggCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGCGCACCGACa                                                   >  2:579485/1‑143 (MQ=255)

                                                                                       ctgcctggCATATCCGCTGCTATGGTCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACa                                                   <  1:693036/143‑1 (MQ=255)

                                                                                         ggctggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGc                                                 >  1:994984/1‑143 (MQ=255)

                                                                                         ggctggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGc                                                 <  1:81417/143‑1 (MQ=255)

                                                                                            tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTCAAGCAGCAATTTTTATATTCATCGTAATATCCCTTTCATTACGTTCCATTACCACCAGATTTCAGCCTGGGCACCGCCAGCaaa                                              >  2:229310/1‑143 (MQ=255)

                                                                                            tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGCCAGCaga                                              >  1:578453/1‑141 (MQ=255)

                                                                                            tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTAAGCCTGGGCACCGACAGCaaa                                              >  1:117820/1‑143 (MQ=255)

                                                                                             ggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAAc                                             >  1:570856/1‑143 (MQ=255)

                                                                                                 tatCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATCAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgt                                         >  2:936730/1‑143 (MQ=255)

                                                                                                  atCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtc                                        >  1:980582/1‑143 (MQ=255)

                                                                                                   tCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAGCTGgtcg                                       >  1:955077/1‑143 (MQ=255)

                                                                                                    cctctgctATGGCCCACTCAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtcgt                                      <  1:298348/140‑1 (MQ=255)

                                                                                                      gctgcgACGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCt                                    <  2:307657/143‑1 (MQ=255)

                                                                                                        tgctATGGCCCACTGAGCAGAAATACCGCTGGCGATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAACCTGGTCGTCttt                                  >  1:698698/1‑143 (MQ=255)

                                                                                                           tagggCCCACTGGGCAGAAGTACCGCTGGCAATAGCGAGCGTTAAAGCAGCAATTTTTATATTCATCGTGATATGCCTTTCAGTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                               <  2:691653/140‑1 (MQ=255)

                                                                                                           tATGGCCCACTGAGCAGAAATGCCGCTGGCAATCGCCAGCGTTAAAGCAGCAATTTTTATCTTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                               <  2:392047/143‑1 (MQ=255)

                                                                                                           tATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                               <  1:974288/143‑1 (MQ=255)

                                                                                                              ggCCCACGGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTc                            <  2:889981/143‑1 (MQ=255)

                                                                                                               gCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCt                           >  1:213960/1‑143 (MQ=255)

                                                                                                                cccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGATCCACTTTATTGTCtt                          >  2:331073/1‑143 (MQ=255)

                                                                                                                 ccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTc                         <  2:701195/143‑1 (MQ=255)

                                                                                                                  cACTGAGCAGAAATACCGCTGGCAATAGCCAGCGCTAAAGCAGCAATTTTCATATTCATCGTAATATTCCTGTCATGAAGTTCCATTACCACCAGATTTCAGCCTGTGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCa                        >  2:1016193/1‑143 (MQ=255)

                                                                                                                      gAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATCTCAGCCTGGGCACCGACAGCAAACTGGTCGACTTTATTGTCGTCAAAgg                    >  2:1004304/1‑143 (MQ=255)

                                                                                                                        gCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTg                  <  2:559326/143‑1 (MQ=255)

                                                                                                                          aGAAATACCGCTGGCAATAGCCAGCTTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGaa                <  2:1953/143‑1 (MQ=255)

                                                                                                                          aGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGaa                <  1:427452/143‑1 (MQ=255)

                                                                                                                           gAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAg               <  1:341675/143‑1 (MQ=255)

                                                                                                                             tttaacGCTGGCTATTGCCAGCGTTACAGCAGCAATTTTTATATTCATCGTAATATTCCTTTTATTAAGTTCCATTATTACTAGATTTCAGACTGGGCACCGACAGCAAACTCGTAGTTTTTATTGTCATGATAGGTCAAGcc             >  2:405392/6‑143 (MQ=255)

                                                                                                                             tttaacGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTGAAAGGTGAAGcc             >  1:728326/6‑143 (MQ=255)

                                                                                                                                aacGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAGGTCCCATTACCACCAGGTTTCAGCCTGGGCACCGACAGCAAACAGGTCGTGTTGATTGTCTTCACAGGTGAAGCCATc          >  1:161725/3‑143 (MQ=255)

                                                                                                                                aacGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGCTTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTATATTGTCTTCCAAGGTGAAGCAATc          >  2:18633/3‑143 (MQ=255)

                                                                                                                                aCCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATc          >  1:183642/1‑143 (MQ=255)

                                                                                                                                aCCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATc          <  2:355155/143‑1 (MQ=255)

                                                                                                                                  cGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCCAAGGTGAAGCCATCCa        >  2:344926/1‑143 (MQ=255)

                                                                                                                                  cGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCa        >  1:105500/1‑143 (MQ=255)

                                                                                                                                  cGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCa        >  1:104630/1‑143 (MQ=255)

                                                                                                                                    cTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGt      <  1:556195/143‑1 (MQ=255)

                                                                                                                                     tGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGTGCACCGACAGCAAACTGGTCGTCTCTATTGTCTTCAAAGGTGAAGCCATCCAGtt     <  2:838560/143‑1 (MQ=255)

                                                                                                                                      ggCAATAGCCAGCGTGAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTc    <  1:704900/143‑1 (MQ=255)

                                                                                                                                       gCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGCTTTCAGCCTGGGCACCGACAGCAACCTGGTCGTCTTTATTGTCTCCAAAGGTGAAGCCACCCAGTTCg   >  2:821276/1‑143 (MQ=255)

                                                                                                                                       gCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTCTTGTCTCCAAAGGGAAAGCCATCCAGTCCg   >  2:830723/1‑143 (MQ=255)

                                                                                                                                        cAATAGCCAGCGTTAAAGCAGCTATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGt  <  2:723497/143‑1 (MQ=255)

CCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAG‑TCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGT  >  NC_000913/3900406‑3900683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`