BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,900,545	A→T	7.5%	A11A (GCT→GCA)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,545	0	A	T	7.5%	366.6 / 19.4	136	A11A (GCT→GCA)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand): ref base A (58/65); new base T (4/6); total (64/72)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-02

Only 100 of 137 total aligned reads displayed.

CCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTTTC  >  NC_000913/3900406‑3900687

   cAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGc                                                                                                                                          <  1:852152/143‑1 (MQ=255)

    aGGGGCAAAGTAGCGAAAGCTCCCGCTGTTCTCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCg                                                                                                                                         <  1:330766/143‑1 (MQ=255)

    aGGGCCAAGGTAGCGAAAGGTGACCATGTTGGCGGCATTGACCTGAGTCACATACTGGTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCg                                                                                                                                         <  2:327813/143‑1 (MQ=255)

     ggggCAGAGTAGCGAAGGGTGACGCTGTTGTCGGCATTGACCTGGGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACGGAGCAGAAATACCGCTGGCTATTGCCAGCgg                                                                                                                                        <  2:990937/143‑2 (MQ=255)

      gggCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGtt                                                                                                                                       >  1:831976/1‑143 (MQ=255)

       ggCAAAGTAGCGAACGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATAACGCTGGCTATTGCCAGCGGTa                                                                                                                                      <  1:337671/143‑1 (MQ=255)

       ggCAAAGTAGCGAAAGGTCACGCTGGTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCGGTa                                                                                                                                      <  2:189502/143‑1 (MQ=255)

        gCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa                                                                                                                                     <  1:961356/143‑1 (MQ=255)

         cAAAGTAGCGAACGGTCACGCTGTTAGCGGCATGGACCTGAGTCACAGACTGTTGCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                    <  1:36248/143‑1 (MQ=255)

          aaaGTAGCGAAAGGTGACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAag                                                                                                                                   <  1:868682/143‑1 (MQ=255)

          aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAag                                                                                                                                   <  1:171046/143‑1 (MQ=255)

          aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCCATGGCCCCCTAAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAag                                                                                                                                   <  1:548232/143‑1 (MQ=255)

          aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGAGCTGGCTGGCATATCCGCTGCTAAGGCCCAGGGAGCAGAACTACCGCTGGCAATAACCAGCGTTAAag                                                                                                                                   >  2:41656/1‑143 (MQ=255)

           aagcggCGAGAGGTCACGCGGTTATGGGCGTTGACCCGAGTCAGATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagc                                                                                                                                  <  2:464545/138‑1 (MQ=255)

            aGTAGCCAAAGGTCACGCCGTTATCGGCATTGACCTGAGTCACATACTGTTGCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagca                                                                                                                                 <  1:472433/143‑1 (MQ=255)

              tAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGAGCTGGCTGGCATATCCGCTGCTATGGCGCACTGAGCAGAAATACCGCTGTCAAGAACCAGCGGTAAatcagc                                                                                                                               >  2:46781/1‑143 (MQ=255)

                  gAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGCGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAtt                                                                                                                           >  1:1021833/1‑143 (MQ=255)

                   aaaGGTCACGCTGTTATCGGGAGTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAttt                                                                                                                          <  2:792234/143‑1 (MQ=255)

                    aaGGTCTCGCGGTTATCGGCATTGGCCTGAGTCACATACTGTTTCACCGGGATTGTGTGTGTCTGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAtttt                                                                                                                         <  2:921007/143‑1 (MQ=255)

                    aaGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCCATAGCCAGCGCTAAAGCAGCAAtctc                                                                                                                         >  2:613439/1‑140 (MQ=255)

                        tCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTtata                                                                                                                     >  2:1032327/1‑143 (MQ=255)

                        tCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGAAATGGCCAGCGTTAAAGCAGCAATTTGtata                                                                                                                     >  1:68743/1‑143 (MQ=255)

                          aCGCTGTCATCGGCACTGACCTGACTCACATACTGTTTCACCGGGATAGTGCGTGCCGGGCTGGCTGGCATCTCCGCTGCTATGGCCCACTTAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATAtt                                                                                                                   <  1:171336/143‑1 (MQ=255)

                             cTGTTAGCGGCATTGACCTGAGTCACATACTGTTTCACCGGGTTCGTGGTGGTCGCTCTGGCTGGCATGTCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCAt                                                                                                                <  2:470642/143‑1 (MQ=255)

                              tGTTATCGGCATTGCCCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATc                                                                                                               <  1:1065286/143‑1 (MQ=255)

                                  aTCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGCTAAAACAGAAACTTTTAAAGTCATCGGaa                                                                                                           >  2:712378/1‑143 (MQ=255)

                                   tCGGCATTGCCCTGAGTCACATACTGTTTCACCGGGATCGTGGGTGCCGGGCTGCCTGGCCTATCCGCTGCTATGGCCCACTAAGCAGCAATACCGCTGGCAATCGCCAGCTGTAAAGCAGCCATTTCTATATCCACCGTCat                                                                                                          >  2:985408/1‑143 (MQ=255)

                                   tCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCAGGCCGGCATATTCGCTTGTTTGGCCCACTGAGCAGCAATACCGCGGGCAATAGCCAGCGTTAAAGCCGCGCTTTTTATATTCATCGTAat                                                                                                          >  1:669852/1‑143 (MQ=255)

                                      gCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATAtt                                                                                                       >  1:102083/1‑143 (MQ=255)

                                      gCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATAtt                                                                                                       <  1:425116/143‑1 (MQ=255)

                                      gCATTGACCTGAGTCACATACTGTTCCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATAtt                                                                                                       <  1:129720/143‑1 (MQ=255)

                                           ggccTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCttt                                                                                                  <  2:25886/141‑1 (MQ=255)

                                            aCCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCGATTTTTAGATGCATCGTAATATTCCTTTc                                                                                                 >  2:503707/1‑143 (MQ=255)

                                               tGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCAtt                                                                                              <  1:684529/143‑1 (MQ=255)

                                               tGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGACGAAATACCGCTGGCAATAGCCAGCGTTAACGCAGCACTTTTTATATTCATCGTAATATTCCTTTCAtt                                                                                              >  2:58232/1‑143 (MQ=255)

                                                gAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCCATTTTTATATTCATCGTACTATCCCTGTGATTa                                                                                             >  2:778557/1‑143 (MQ=255)

                                                 aGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTCAAGCAGCAATTTCTATATTCATCGTAATATTCCTTTCATTaa                                                                                            <  1:1065771/143‑1 (MQ=255)

                                                 aGTCACATACTGTTTCACCGGGATAGTGGGGGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTaa                                                                                            <  2:723083/143‑1 (MQ=255)

                                                    cacaTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAAGACCGCTGGCAATAGCCAGCGTTAAAGCACCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGtt                                                                                         <  1:227422/143‑1 (MQ=255)

                                                       aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGGTGGCATATCCGCTGTTATGCCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCa                                                                                      <  1:1055408/143‑1 (MQ=255)

                                                       aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCa                                                                                      <  1:871047/143‑1 (MQ=255)

                                                       aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACGGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAGAGCAGCCATATTTCTAGTCATCGTAATATTCCTGTCATTAAGCTCCa                                                                                      >  2:727228/1‑143 (MQ=255)

                                                        tACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCACCAATTTTTATATTCATCGTAATATTCCTTTCATTCAGTTCCac                                                                                     >  2:714488/1‑142 (MQ=255)

                                                         aCTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTGATATTCCTTTCATTAAGTTCCAtt                                                                                    >  1:209935/1‑143 (MQ=255)

                                                             tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTacca                                                                                >  1:701195/1‑143 (MQ=255)

                                                                cACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTAccacca                                                                             >  1:559326/1‑143 (MQ=255)

                                                                 aCCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATGTTCCTTTCATTAAGTTACAATaccacccg                                                                            >  2:307598/1‑141 (MQ=255)

                                                                       aTAGTTGGTGCCGTGCTGGCTGGCATATCCGCTGCTATGGCCCACTGATCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCa                                                                      <  2:63036/143‑1 (MQ=255)

                                                                         aGTGGGTGCCGGGCTGGCTGGCATAGCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATGCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGc                                                                    <  1:503875/143‑1 (MQ=255)

                                                                          gTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTCCAGTTAGTTCCATTACCACCAGATTTCAGcc                                                                   >  1:1001003/1‑143 (MQ=255)

                                                                           tGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCt                                                                  <  2:24806/143‑1 (MQ=255)

                                                                            gggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCCGATTTCAGCCTg                                                                 >  1:280894/1‑143 (MQ=255)

                                                                            gggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTg                                                                 <  2:711247/143‑1 (MQ=255)

                                                                             ggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTgg                                                                <  2:57709/143‑1 (MQ=255)

                                                                              gggCCGGGCTGGCTGGCATAGCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                               <  1:199852/141‑1 (MQ=255)

                                                                              gTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATGGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                               <  1:520598/143‑1 (MQ=255)

                                                                              gTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                               >  1:8618/1‑143 (MQ=255)

                                                                               tGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTCAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggac                                                              >  1:595818/1‑141 (MQ=255)

                                                                               tGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGc                                                              >  1:251345/1‑143 (MQ=255)

                                                                                    ggctggctGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTGTCTTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGa                                                         <  1:446336/143‑1 (MQ=255)

                                                                                    ggctggctGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATGCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGa                                                         <  1:743192/143‑1 (MQ=255)

                                                                                        ggctggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGc                                                     >  1:994984/1‑143 (MQ=255)

                                                                                        ggctggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGc                                                     <  1:81417/143‑1 (MQ=255)

                                                                                           tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTAAGCCTGGGCACCGACAGCaaa                                                  >  1:117820/1‑143 (MQ=255)

                                                                                            ggcATCTCCGCCGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAAc                                                 <  2:202506/143‑1 (MQ=255)

                                                                                            ggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAAc                                                 >  1:570856/1‑143 (MQ=255)

                                                                                                tatCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATCAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgt                                             >  2:936730/1‑143 (MQ=255)

                                                                                                  tCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAGCTGgtcg                                           >  1:955077/1‑143 (MQ=255)

                                                                                                   cctctgctATGGCCCACTCAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtcgt                                          <  1:298348/140‑1 (MQ=255)

                                                                                                     gctgcgACGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCt                                        <  2:307657/143‑1 (MQ=255)

                                                                                                       tgctATGGCCCACTGAGCAGAAATACCGCTGGCGATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAACCTGGTCGTCttt                                      >  1:698698/1‑143 (MQ=255)

                                                                                                          tagggCCCACTGGGCAGAAGTACCGCTGGCAATAGCGAGCGTTAAAGCAGCAATTTTTATATTCATCGTGATATGCCTTTCAGTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                                   <  2:691653/140‑1 (MQ=255)

                                                                                                          tATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAtt                                   <  1:974288/143‑1 (MQ=255)

                                                                                                             ggCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTc                                <  1:560499/143‑1 (MQ=255)

                                                                                                             ggCCCACGGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTc                                <  2:889981/143‑1 (MQ=255)

                                                                                                              gCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCt                               >  1:213960/1‑143 (MQ=255)

                                                                                                                ccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTc                             <  2:701195/143‑1 (MQ=255)

                                                                                                                 cACTGAGCAGAAATACCGCTGGCAATAGCCAGCGCTAAAGCAGCAATTTTCATATTCATCGTAATATTCCTGTCATGAAGTTCCATTACCACCAGATTTCAGCCTGTGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCa                            >  2:1016193/1‑143 (MQ=255)

                                                                                                                   cgtgccggAAATTACGCAGGCAATAGCCAGCGTTAAAGCAGCACTTTTTATCTTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGCTTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCaaa                          <  2:165966/136‑1 (MQ=255)

                                                                                                                   cTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCaaa                          <  1:700289/143‑1 (MQ=255)

                                                                                                                    tGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAg                         <  1:613439/143‑1 (MQ=255)

                                                                                                                     gAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTACATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGCCAGCAAACAGGTCGACTTTATTGTCTTCAAAgg                        >  2:1002547/1‑143 (MQ=255)

                                                                                                                       gCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTCTATTCATCGTAATATTCCTTTCATTAAATTCCATTACAACCAGATTTCAATCTGAGCACCAACAGCAAACTGGACGGCTTCATTGTCTTCAAAggag                      >  2:366357/1‑141 (MQ=255)

                                                                                                                       gCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTg                      <  2:559326/143‑1 (MQ=255)

                                                                                                                         aGAAATACCGCTGGCAATAGCCAGCTTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGaa                    <  2:1953/143‑1 (MQ=255)

                                                                                                                          gAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAg                   <  1:341675/143‑1 (MQ=255)

                                                                                                                            tttaacGCTGGCTATTGCCAGCGTTACAGCAGCAATTTTTATATTCATCGTAATATTCCTTTTATTAAGTTCCATTATTACTAGATTTCAGACTGGGCACCGACAGCAAACTCGTAGTTTTTATTGTCATGATAGGTCAAGcc                 >  2:405392/6‑143 (MQ=255)

                                                                                                                            tttaacGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTGAAAGGTGAAGcc                 >  1:728326/6‑143 (MQ=255)

                                                                                                                               aacGCTGGCTATTGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAGGTCCCATTACCACCAGGTTTCAGCCTGGGCACCGACAGCAAACAGGTCGTGTTGATTGTCTTCACAGGTGAAGCCATc              >  1:161725/3‑143 (MQ=255)

                                                                                                                               aCCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATc              <  2:355155/143‑1 (MQ=255)

                                                                                                                               aCCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATc              >  1:183642/1‑143 (MQ=255)

                                                                                                                                 cGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCCAAGGTGAAGCCATCCa            >  2:344926/1‑143 (MQ=255)

                                                                                                                                 cGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCa            >  1:104630/1‑143 (MQ=255)

                                                                                                                                 cGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCa            >  1:105500/1‑143 (MQ=255)

                                                                                                                                    tGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGTGCACCGACAGCAAACTGGTCGTCTCTATTGTCTTCAAAGGTGAAGCCATCCAGtt         <  2:838560/143‑1 (MQ=255)

                                                                                                                                      gCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGCTTTCAGCCTGGGCACCGACAGCAACCTGGTCGTCTTTATTGTCTCCAAAGGTGAAGCCACCCAGTTCg       >  2:821276/1‑143 (MQ=255)

                                                                                                                                      gCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTCTTGTCTCCAAAGGGAAAGCCATCCAGTCCg       >  2:830723/1‑143 (MQ=255)

                                                                                                                                      gCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCg       <  1:275994/143‑1 (MQ=255)

                                                                                                                                       cAATAGCCAGCGTTAAAGCAGCTATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGt      <  2:723497/143‑1 (MQ=255)

                                                                                                                                           aGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCGATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCGAGTTCGTTTTc  >  1:386238/1‑143 (MQ=255)

CCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTTTC  >  NC_000913/3900406‑3900687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`