BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,976,399	C→A	5.1%	A41E (GCA→GAA)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,399	0	C	A	5.1%	265.6 / 2.3	102	A41E (GCA→GAA)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand): ref base C (48/43); new base A (3/2); total (55/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 109 total aligned reads displayed.

GTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACT  >  NC_000913/3976258‑3976540

gttgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGATGTCATTTGGTCCCGCGGGGCTTGGGCTGGCGGCa                                                                                                                                               >  2:531923/1‑143 (MQ=255)

  tgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACGGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCCGTGTCATTGGGTCCGGCGGGGCTGGGGGGGGCGGCaag                                                                                                                                             >  2:762436/1‑142 (MQ=255)

   taacgtctTTTCCTGGTATGGCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGAGTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAAt                                                                                                                                            <  2:505897/137‑1 (MQ=255)

    aaCTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGTACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGGTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAAtt                                                                                                                                           <  2:1059556/143‑1 (MQ=255)

      ctgctTTTCCTTATATGTCGTGGGCAAAAGCGTCTTTGTGGACGGCGGCCAGTACGCTGGTCAAGATTGGTGCCGGGGTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑c                                                                                                                                        <  2:105082/140‑1 (MQ=255)

      ctactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑c                                                                                                                                        >  1:577021/1‑143 (MQ=255)

       tactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATGGGTGCCGGGTTACTGGTCGGTACGTTGCTGGCGGTGTGCTTTGGTCGGGCGGGGAGTGGGCTGGCGGCAAGTTT‑cg                                                                                                                                       >  2:802615/1‑142 (MQ=255)

         ctTTTCCTGATATGTCGTTGGCAAAATCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGGCATTTGGTCCAGCAGGGCTTGGGCTTGAGGGAAATGT‑CAGc                                                                                                                                     >  1:558481/1‑143 (MQ=255)

            ttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGAGTCATTTGGTCCGGCGGGGCGTGGGTTGGCGGCGAATTT‑ccccaag                                                                                                                                  >  2:481831/1‑138 (MQ=255)

             tCCTGGTATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTTGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTG‑CCGCCAgc                                                                                                                                 <  2:194654/143‑2 (MQ=255)

              ccTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGtt                                                                                                                                <  1:873383/143‑1 (MQ=255)

              ccTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGtt                                                                                                                                <  2:824403/143‑1 (MQ=255)

              ccTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGtt                                                                                                                                <  2:601646/143‑1 (MQ=255)

              ccTGATACGTCGTTGGCCAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGtt                                                                                                                                <  1:426613/143‑1 (MQ=255)

                 gATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACGCTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGAt                                                                                                                             >  2:853107/1‑143 (MQ=255)

                   tatGTCGTTGGCAAGAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTa                                                                                                                           <  2:201066/143‑1 (MQ=255)

                          ttGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCGAGTTGATTACCGTGCt                                                                                                                    >  1:303161/1‑143 (MQ=255)

                          ttGGCAAAAGCGTCCGTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACAGATCGATAAGTTGCTAACGATGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCt                                                                                                                    >  1:979098/1‑143 (MQ=255)

                            ggCAAGAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCg                                                                                                                  <  1:994697/143‑1 (MQ=255)

                             gCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCACATCT‑CCGCCAGTTGATTACCGTGCTctg                                                                                                                 >  1:997756/1‑141 (MQ=255)

                             gCAAAAGCGTCCGTGTGGACGGCGGCCAGTACACTGGTCAAGATGGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCgg                                                                                                                 <  1:557007/143‑1 (MQ=255)

                               aaaaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCg                                                                                                               >  1:766080/1‑143 (MQ=255)

                               aaaaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCg                                                                                                               >  1:457810/1‑143 (MQ=255)

                               aaaaGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCGGGCGGTGTCATTTGGTCCGGCGGGGCGTGGGCTGGCGGCAGATTT‑CGGCCCGTTGATGAGCGGGCTCGGCg                                                                                                               >  2:407425/1‑143 (MQ=255)

                                    cGTCCTGGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCtt                                                                                                          <  1:122331/143‑1 (MQ=255)

                                       ccTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCGTTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAGTTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGcc                                                                                                       >  1:823903/1‑143 (MQ=255)

                                         ttGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCgg                                                                                                     >  1:432574/1‑143 (MQ=255)

                                           gtgGACGGCGGCCAGTGCACTGGGCGAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCgggg                                                                                                   <  2:817470/143‑1 (MQ=255)

                                               aCGGCGGCCAGTACGCTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                                                                               <  1:827060/143‑1 (MQ=255)

                                                  gcggcgTGTACGCGGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGGTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTGGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCAt                                                                                            <  2:316762/143‑1 (MQ=255)

                                                  gcggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCACGTGGTCCGGCGGGGCTGGGGCTGGCGGCCAAGTT‑CCGCCAGTTGGTTACAGTGCTCGGAGTGCTTGGCGGGGCTGGCAt                                                                                            >  2:848715/1‑143 (MQ=255)

                                                   cggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAGTTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCCTc                                                                                           >  2:738001/1‑143 (MQ=255)

                                                    ggcCAGTGCACTGGTCAAGAGTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                                                                          <  1:721696/143‑1 (MQ=255)

                                                    ggcCAGTACACTGGTCAAGATTGGTGCCGGGATACTGGTCGGTAAGTTGCTCGTAGCCTGATGTGGTCCGGCGAGGCTCGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                                                                          >  2:754280/1‑143 (MQ=255)

                                                         gtccgcTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAAc                                                                                     <  1:670278/138‑1 (MQ=255)

                                                          tACGCTGGTCATGATGGGTGGCGGGTTGCTGGTCGGTAGGGTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACg                                                                                    <  2:705845/143‑1 (MQ=255)

                                                          tACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACg                                                                                    <  1:270500/143‑1 (MQ=255)

                                                            cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                                                                                  <  1:853107/143‑1 (MQ=255)

                                                            cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                                                                                  >  1:43044/1‑143 (MQ=255)

                                                               tgtcgaaGTTTGGTGGCGGGTGACGGGTTGGTATGTTGGTCTGGGTGTTATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAATTTT‑CCGCCTGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGTTGGCATCTTTAACGGTGTa                                                                               <  2:770002/138‑1 (MQ=255)

                                                                ggTCAAGATTGGGGCCGGGTTACCGGTCCGGAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTaa                                                                              <  1:717492/143‑1 (MQ=255)

                                                                  tctcgagtggtggCGTGTTACGGGTCGGTGTGGTGGTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAAcc                                                                            <  2:410969/136‑1 (MQ=255)

                                                                    aaGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCaa                                                                          >  1:575065/1‑143 (MQ=255)

                                                                     cgATTGGTGCCGGGTTACGGGTCGGGTAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAAGTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCaaa                                                                         <  2:1057670/142‑1 (MQ=255)

                                                                     aGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCaaa                                                                         >  1:316242/1‑143 (MQ=255)

                                                                     aGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGAGGCAGGGATCTTTAACGGTGTAACCcaa                                                                         >  2:258096/1‑143 (MQ=255)

                                                                       agtGGTGCCGGGTTGCTGGTCGGTGAGGTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGTCATCTTTAACGGTGTAACCAAATa                                                                       <  2:930267/141‑1 (MQ=255)

                                                                         tGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACg                                                                     <  1:357075/143‑1 (MQ=255)

                                                                          ggTGCCGGGTGAGTGGCTGGTGGGTTGCGGGCGGTGTCGTTTGGTCCGGCGGGGCTTGGGCTGGGGGTAAATTT‑CCGCCAGTTGATTTCCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTGATCGGTGTAACCAAATACGt                                                                    <  2:272389/143‑1 (MQ=255)

                                                                            tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCCAATTT‑CGGCCAGTTGATTACCGGGCTCGGCGTGATTGCAGGGGCTGGCATCTTGCACGGTGTAAGCAAATACgggc                                                                  >  2:885928/1‑140 (MQ=255)

                                                                             gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTCACCAAATACGTTGc                                                                 >  1:952121/1‑143 (MQ=255)

                                                                              ccGGGTTACTGGTCGGTAGGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                                                >  2:484439/1‑143 (MQ=255)

                                                                              ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGGCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcg                                                                >  1:275617/1‑142 (MQ=255)

                                                                              ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                                                <  1:871433/143‑1 (MQ=255)

                                                                               cGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCACATACGTTGcac                                                               >  2:295421/1‑141 (MQ=255)

                                                                                ggcttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGCTGATCACCGTGCTCGTCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCa                                                              <  1:573603/140‑1 (MQ=255)

                                                                                   ttACTGTTCGGTAAGTTGCGGGCGGTGTCATCTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTCATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa                                                           <  1:228024/143‑1 (MQ=255)

                                                                                    tACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTAc                                                          <  1:606225/143‑1 (MQ=255)

                                                                                         gTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCCACTTT‑CCGCCCGTTCATTACCGTGCCCGGCGTGCTTGCCGCGGCTGGCATCTTTAACGGCGTAACCAAATACATTGCCCAGTACCATGa                                                     >  1:177005/1‑143 (MQ=255)

                                                                                          tCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCGGCAATTTACTGCTGTTTCGGTGTGGCCGAAGACGTTGCCCAGGACCATGAt                                                    >  1:238174/1‑143 (MQ=255)

                                                                                          tCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGAt                                                    <  1:796262/143‑1 (MQ=255)

                                                                                             ctAAGTTGCTGGCGGTGTCATTTGATCCGGCGGGGCTCGGGCTGACGGCAAATTC‑GCGACAGTAGATCACAGTGCTCCGCGTGCTTGCCGGGGCTGGCATCTACAAACGTGTAAACAAATACGTTGCGCGGCACCACGACaac                                                 >  2:1015563/2‑142 (MQ=255)

                                                                                              tAAGGTGCTGGCTGTGTCATTTGGTCGGGCGTGTGTTGGGCGTGCGGGTAATTT‑CTGCCGGTTGATTTCGGTGCTCGGCGTGCTTTCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATc                                                <  2:511802/143‑1 (MQ=255)

                                                                                                aGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGCTTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCACCCTTAACGGTGTAACGAAGTGCGTTGCCCAGTAGCAGGGTGATCCg                                              >  2:80339/1‑143 (MQ=255)

                                                                                                aGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCg                                              <  1:502829/143‑1 (MQ=255)

                                                                                                  ttGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCa                                            >  2:933498/1‑143 (MQ=255)

                                                                                                      tGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                                                <  2:229826/119‑1 (MQ=255)

                                                                                                      tGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGcc                                                                >  1:229826/1‑119 (MQ=255)

                                                                                                      tGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAg                                        <  2:316242/143‑1 (MQ=255)

                                                                                                      tGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCAGCTTTAACGGTGTAACCAAATACGTTGCCCGGTACCATGGTACTCCGCAACac                                        >  1:409448/1‑142 (MQ=255)

                                                                                                      tGGCGGTGCCATCTGGTCCTGCTGGGCTTGGTCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAg                                        <  2:772290/143‑1 (MQ=255)

                                                                                                               cATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGcgccgc                               <  2:303161/143‑1 (MQ=255)

                                                                                                                aTTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                               <  1:475644/126‑1 (MQ=255)

                                                                                                                aTTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                               >  2:475644/1‑126 (MQ=255)

                                                                                                                 tttgtcccGGCGGGGCTGGGGCTGGCGGCAAATGG‑CCGCCAGTTGTTTACGGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTCTAACGGTGTAACCAGATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGt                             <  2:558481/137‑1 (MQ=255)

                                                                                                                 tttGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGt                             >  1:746821/1‑143 (MQ=255)

                                                                                                                  ttGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTg                            >  1:1021510/1‑143 (MQ=255)

                                                                                                                    ggTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCCTGATCATCCGCAACAGCTGCGCCGCGGGGt                          >  1:416686/1‑143 (MQ=255)

                                                                                                                    ggTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGt                          <  1:653103/143‑1 (MQ=255)

                                                                                                                     gTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTc                         >  2:762852/1‑143 (MQ=255)

                                                                                                                      tCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCg                        >  1:759959/1‑143 (MQ=255)

                                                                                                                       ccGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCgg                       <  2:432574/143‑1 (MQ=255)

                                                                                                                         ggcggGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCGCGGCCTGCTTGCCGGGGCTGGCATCTATAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGggcggcc                     >  2:90197/1‑142 (MQ=255)

                                                                                                                              ggCTTGGGCTGGCGGCCAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGGATCGTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATACTCCGCACCAGCTGTGCCGCGTGGTCGGCACTtca                <  2:576553/143‑1 (MQ=255)

                                                                                                                              ggCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCGCTtca                >  1:924856/1‑143 (MQ=255)

                                                                                                                                 ttGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAACTACGTTGCCCAGTACCATGATACTCCGCAACAGCTGCGCCGCGTGGTCGGCACCtcgtca             >  2:313123/1‑143 (MQ=255)

                                                                                                                                 ttGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTtcatca             >  1:1028585/1‑143 (MQ=255)

                                                                                                                                  ggggCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAg            <  1:820710/142‑1 (MQ=255)

                                                                                                                                      cTGGCGGACAATTT‑CCGCCAGTGGATTACCGTGCTCGGGGTGCTGGCCGGGGCTGCCATCTTTAACTGGGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGAt        <  2:438715/143‑1 (MQ=255)

                                                                                                                                      cTGGCGGAAATTTG‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGCGGTCGGCACTTCATCAGCGag        >  2:547563/1‑142 (MQ=255)

                                                                                                                                      cTGGCGGAAATTTG‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGAGGAACCAAATACGTTGCCCAGTACCCTGATAATCCGCAACCGCTGCGCCGCGTGGTCGGCACTTCATCAGCGag        >  1:346254/1‑142 (MQ=255)

                                                                                                                                      cTGGCGG‑AAATTTGCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCgct        >  1:916812/1‑141 (MQ=255)

                                                                                                                                       tGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCCGCGATg       >  2:100149/1‑143 (MQ=255)

                                                                                                                                         gcggcAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGCGTAGCCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCCTCCTCGGCCCTTCATCAACGaatgt     >  1:164763/1‑139 (MQ=255)

                                                                                                                                         gcggcAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGCGTAACGAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGt     >  1:668294/1‑143 (MQ=255)

                                                                                                                                          cggcAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGATGTAACAAGATACGTTGCCCAGTACCATGATGATCCGCAACAGCTGCGCGGCGTGGTCGGCACTTCACCAGCGGTGGTa    >  1:418482/1‑143 (MQ=255)

                                                                                                                                          cggcAAATTT‑CCGCCAGTGGATTACCGTGCTCGGCGGGCGTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTa    <  2:584019/143‑1 (MQ=255)

                                                                                                                                           ggcAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTAc   >  1:771189/1‑143 (MQ=255)

                                                                                                                                            gcAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGGGGTCGGCACTTTATCGGCGAGGGTACt  >  2:454590/1‑143 (MQ=255)

                                                                                                                                            gcAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCCTGGTCGGCACCTCATCACCAATGGCACt  >  2:187208/1‑143 (MQ=255)

GTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTT‑CCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACT  >  NC_000913/3976258‑3976540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`