Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 464,356 A→T 6.1% intergenic (+48/‑46) ybaV → / → fadM helix‑hairpin‑helix 3 family protein/thioesterase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913464,3560AT 6.1% 199.5 / 4.0 66intergenic (+48/‑46)ybaV/fadMhelix‑hairpin‑helix 3 family protein/thioesterase III
Reads supporting (aligned to +/- strand):  ref base A (28/33);  new base T (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGG  >  NC_000913/464217‑464497
                                                                                                                                           |                                                                                                                                             
aCGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGtt                                                                                                                                            <  1:833424/143‑1 (MQ=255)
 cGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATCGTGGCACTTTTTTGCCCGACTGAAGATGTCCCATCAGttc                                                                                                                                           >  2:162595/1‑142 (MQ=255)
  ggTCCGTTTAAAGCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                                                                                                          <  1:632309/143‑1 (MQ=255)
    tCCGTTTAGAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGGAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGa                                                                                                                                        >  2:650061/1‑143 (MQ=255)
    tCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGa                                                                                                                                        >  2:1053177/1‑143 (MQ=255)
    tCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGAGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGa                                                                                                                                        >  1:875558/1‑143 (MQ=255)
     ccGTTTAAAACTGTGGAGGATCTCAAGCAGGTGCCGGGGATGAGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCCGTTATgca                                                                                                                                       >  2:928787/1‑141 (MQ=255)
       gtgtAAAGCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACct                                                                                                                                     <  2:951757/140‑1 (MQ=255)
        tttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACGCTGTCATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTAATACCAGTTATGACctc                                                                                                                                    >  1:1039032/1‑143 (MQ=255)
          tAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctctt                                                                                                                                  <  1:1053177/143‑2 (MQ=255)
            agaCTGTGGACGATCTAAAGCAGGTGCCGGGGATGGGCAATTCCCAGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTa                                                                                                                                <  1:39778/141‑1 (MQ=255)
                   ggaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaa                                                                                                                         <  1:173893/143‑1 (MQ=255)
                   ggaggaGCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaa                                                                                                                         <  1:757887/143‑1 (MQ=255)
                    gaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAGCACTTATGACCTCTGTACTTATaac                                                                                                                        >  2:899294/1‑143 (MQ=255)
                    gaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaac                                                                                                                        >  1:628426/1‑143 (MQ=255)
                       gaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTCATaccaac                                                                                                                     >  1:87893/1‑143 (MQ=255)
                         tCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaggaa                                                                                                                   >  2:111940/1‑139 (MQ=255)
                          ctaagtgaGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                  <  2:877179/136‑1 (MQ=255)
                                        ggggATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGGATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcg                                                                                                    <  1:529238/143‑1 (MQ=255)
                                           gATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTa                                                                                                 <  1:814486/143‑1 (MQ=255)
                                             tgtgCAATTAGCTGCTGCAACCTAATCTGTCATTATTAACCCTGTAAGTCATTTGCATAATAGCAATTTTTTCCCACACTGAACAAGTCATACCAGTTATGACCTCTATACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                                                               <  2:179509/140‑1 (MQ=255)
                                             tGGTCAATTCGTTGGTGGAACGTAATTTGGCGGTATTAACCCTTTACTTAATTTGCATAGTGGAAATGTTTTGCCAGACTGAAGAGGTCATACCAGTTATGCCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                                                               <  2:870692/143‑1 (MQ=255)
                                             tGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCGGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                                                               <  1:108311/143‑1 (MQ=255)
                                                 cAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTCTTGCCAGACTGAAGAGGTCATACCAGTTATGAGCTCTGTACTTATCACAACAACGTAAGGGTATTGCGCGATGCaaa                                                                                           >  2:217433/1‑143 (MQ=255)
                                                    ttCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCCATTTTTTGCCATACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAacac                                                                                        >  1:953070/1‑143 (MQ=255)
                                                    ttCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCACTTTTTTGCCCGACTGCAGAGGTCATACCATTTATGACCTCTGTAATTATAACAACAACGTAAGGTTATTGCACTATGCAAacac                                                                                        >  1:277316/1‑143 (MQ=255)
                                                      cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACaa                                                                                      <  1:809398/143‑1 (MQ=255)
                                                         tggtggAACGTCATCTGGCGGTATTAACCCTGTAATTCATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGCGGTCATACCATTTATGACCTCTGTAGGTACAACAACCACGTAAGGTTATTGCGCTAGGCAAACACAAATc                                                                                   >  2:25786/1‑143 (MQ=255)
                                                         tggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATc                                                                                   >  1:639504/1‑143 (MQ=255)
                                                            tgtaaCGTAATCTGGCGGGATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGTCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCaaa                                                                                <  1:549859/140‑1 (MQ=255)
                                                            tgcatagTAAGCTTGCGGTATTAACCCTGTAATTCATTTGCAGAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCaaa                                                                                <  2:956310/137‑1 (MQ=255)
                                                                  gTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGt                                                                          <  2:1063348/143‑1 (MQ=255)
                                                                  gTAATCTGGCGGTATTAACCCTGTAATTAAGTGGCATAGTGGCAAGTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGt                                                                          <  1:158710/143‑1 (MQ=255)
                                                                   tAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTg                                                                         <  1:879184/143‑1 (MQ=255)
                                                                    aaTCTGGCGGTATTAACCCCGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACATCAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTgg                                                                        <  1:231402/143‑1 (MQ=255)
                                                                       cTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGata                                                                     <  1:290118/143‑1 (MQ=255)
                                                                          gCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTGCGTGGATatca                                                                  >  1:527373/1‑143 (MQ=255)
                                                                                  aaCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAGACACAAACCAAAGTTAGTAGATATCGtctccgcg                                                          >  2:445999/1‑139 (MQ=255)
                                                                                    cccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACCACAACGTAAGGTTATTGCGCTATGCAAACACACCTCAAAGTTCGTGGCTATCCTCTCGACCtt                                                        >  1:115586/1‑143 (MQ=255)
                                                                                    cccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGtt                                                        >  2:263706/1‑143 (MQ=255)
                                                                                     ccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCCTCTCGACGatt                                                       >  2:21982/1‑143 (MQ=255)
                                                                                     ccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAAGCAAAGTTCGTGGATATCATCTCGACGtgt                                                       >  1:690900/1‑141 (MQ=255)
                                                                                       tGTCATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAc                                                     <  1:263748/143‑1 (MQ=255)
                                                                                         taattaatTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAcaa                                                   >  2:623009/1‑141 (MQ=255)
                                                                                           attaattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGc                                                 >  2:32471/1‑143 (MQ=255)
                                                                                                  tGCATAGTGGAAATTTTTTGCCAGACTGAAGAGGTGATACCAGTTATGACCTGTGTACTTATAACAACAACGTAGTGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTcaa                                          <  2:586902/143‑1 (MQ=255)
                                                                                                         tGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGcc                                   <  1:901198/143‑1 (MQ=255)
                                                                                                          ggCAAGTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGccc                                  <  2:875558/143‑1 (MQ=255)
                                                                                                           gCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTCGATCTCGTCTCCACGTTTACCAGCACGGTAAAAACGCCCg                                 >  1:631139/1‑143 (MQ=255)
                                                                                                             aaTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCACCCACccccccg                               >  1:652423/1‑140 (MQ=255)
                                                                                                                     gCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGaa                       <  1:898675/143‑1 (MQ=255)
                                                                                                                      ccAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTACTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAAt                      <  2:506301/143‑1 (MQ=255)
                                                                                                                       cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACACCGTAAGGTTATTGCGCTATGCAACCACAAATCAAAGTTCGTGGATATCGTCTCGACGTTTACCAGCACGTCAACACCGCCCGCTACCCGGAAtt                     >  1:313803/1‑143 (MQ=255)
                                                                                                                         aagtacagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtc                   >  1:23833/7‑143 (MQ=255)
                                                                                                                          acggAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtct                  <  1:650061/140‑1 (MQ=255)
                                                                                                                           cTGAAGAGGTCATACCAGTTATGAACTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtctc                 <  1:822945/143‑1 (MQ=255)
                                                                                                                              aaGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgag              <  1:178216/143‑2 (MQ=255)
                                                                                                                               agagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaag             >  2:820807/1‑143 (MQ=255)
                                                                                                                               agagGTCATACCAGTTATGACCGCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaag             <  2:377542/143‑1 (MQ=255)
                                                                                                                                   gTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGc         >  1:904555/1‑143 (MQ=255)
                                                                                                                                   gTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATAGCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGc         <  1:978490/143‑1 (MQ=255)
                                                                                                                                     cATACCAGTTATCGCCGCTGTACTTCTAACAACCTCGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGccc       <  2:312318/143‑1 (MQ=255)
                                                                                                                                      aTACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGGCGTATACCAGCACGTCAACAACGCCCGCAACCTTGAATTTCTCCAAGAAGccgg      >  2:121228/1‑141 (MQ=255)
                                                                                                                                      aTACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAACAAGCCCg      >  2:622417/1‑143 (MQ=255)
                                                                                                                                         ccAGTTACGACCTGTGTACATCTGCCAACACCGGAAGGTGATTGCGCTATGCAAACACAAATCAAAGTTCGCGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTg   <  2:305299/143‑1 (MQ=255)
                                                                                                                                          cAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTgg  <  1:111940/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
ACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGG  >  NC_000913/464217‑464497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑