| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,085 | T→A | I690N (ATC→AAC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | A | 97.3% | 97.2 / NA | 37 | I690N (ATC→AAC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (16/20); minor base C (0/1); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124947‑125220 | gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCAGGACCGTGAAGACGGTGAAAAACAAGAGAACGTTTAATACTACAACACt > 2:161263/1‑143 (MQ=255) agaTTCGGTCGCTCACTATCCCGAGCTGTATCTCTTACGTCCCGGCTGACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAAc < 2:698610/141‑1 (MQ=255) ttCAGCTGCTGACTATCCCGAACTATATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGaa < 2:353087/143‑1 (MQ=255) gTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACt > 2:745238/1‑143 (MQ=255) gtgtgTCTCTTACGACCCGGCTTACGCGTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGc < 2:127459/142‑1 (MQ=255) tctcTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGaa < 1:745384/143‑1 (MQ=255) ctctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 1:552253/143‑1 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGACAGGCCggctggtt > 2:40256/1‑137 (MQ=255) gACCCGGCTGACGCGTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa < 1:627202/143‑1 (MQ=255) ggCTGACGCTTACGACGTGGCTGTCATCGTGGATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 2:157932/143‑1 (MQ=255) acgcttacgAAGTTGCTGTCATCATGCAGGACGGTCTGGAGCGTATGGACGGGGAAAAACAAGAGAACGTTTACTACGACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATc < 1:194978/143‑1 (MQ=255) aggAAGTTGCTGTCATCATGCATGACGGGCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaaa < 1:388937/141‑1 (MQ=255) acgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGACAAACAAGAGAACGTTTACTACTACAACCCTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGCTAACCGGaac > 2:65594/1‑142 (MQ=255) cgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 1:398127/143‑1 (MQ=255) gAAGTTGCTGTCATCCTGCATGACGGTCTGGAGCCTATGTACGGTGAAAAACAAGAGAACGTTTACTACTAGCACACTGCGCTGAGCGAGAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAgg < 2:564818/143‑1 (MQ=255) ttGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTCAGGAAGGTATCCGTAAAGATATc > 1:369762/1‑143 (MQ=255) cTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAGGGTATCCGTAAAGGTATCTAc > 1:490066/1‑143 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCACTGCCGGAAGGTGTTGTGGAAGTTATCCGTAAAGAAAACTACa > 1:266202/1‑143 (MQ=255) gTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa > 1:27272/1‑143 (MQ=255) tcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTGTCGGTAAAGGTATCTACAAACTc > 1:776410/1‑143 (MQ=255) tcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTc < 1:557789/143‑1 (MQ=255) gTCTGGCGCGTATGTACGGTGAACAACAAGACACCTTTTACTACTCCAACACTCCGCTGAACGACAACTACCACATGCCGGCAATGCCGGAAGGTGCTGGCCTGTTTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:237749/143‑1 (MQ=255) gTCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 1:377548/143‑1 (MQ=255) gTCTGGAGCGTATGGACGGTGAAAAACAGGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:276838/143‑1 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTAGAAACTCGAAACTATTGAAGGt > 2:589879/1‑143 (MQ=255) aGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCCCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGc < 1:50163/143‑1 (MQ=255) tGTACGGTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGt < 1:432330/143‑1 (MQ=255) tACGGTGAAAAACAAGAGAACGTTTCCTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaa < 2:286312/143‑1 (MQ=255) cGGTGAAAAACAAGAGAACGTCCACTACTACAACAATACCCCGAACCAAAACCACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAg < 1:485713/143‑1 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGCAGGTAGCAAAGGTAAAGt > 1:712570/1‑143 (MQ=255) aaacccAAGAGAACGTTTACTACTACAACACGACGCTGAACGAAAACTACCACATGTCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAATGTAGCAAAGGTAAAGTTCAg < 2:199676/138‑1 (MQ=255) aaCAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACCACCACATGCCGGCACTGCCGGAAGGTGCTGAGGAAGGTATCCGCAAAGGTGTCTCCAAGCTCGCAACTATAGACGGAAGCCCAGGTACAGTTCAcctc > 2:627306/1‑142 (MQ=255) cAAGAGAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAGTGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAGGGTAGCGAAGGTAAAGTTCAgctggt > 1:147353/1‑141 (MQ=255) agagAACGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACCAAACGGATATTACTGATGGTAGCAAAGGTAAAGTTCAGCTGCTCg > 1:530229/1‑143 (MQ=255) gaACGTTTACTACTACAACACTACGATGAACGAAAACTACCACATGCCGGCAATGCAGACAGGTGCAGAGGAAGGTATCCGTAAAGGTATCTACAAACTCCAAACCATTGAAGCACGCAACGGTCTAGTCAAGCTGcgcgccc > 2:768283/1‑140 (MQ=255) aCGTTTACTACTACAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTcc > 2:81105/1‑143 (MQ=255) tactacAACACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAt < 2:27272/143‑1 (MQ=255) | GTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124947‑125220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |